Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,515,631	A→G	K18K (AAA→AAG)	patD →	gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,515,631	0	A	G	100.0%	249.7 / NA	66	K18K (AAA→AAG)	patD	gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/28);  total (38/28)

TAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAAAGTG  >  NC_000913/1515495‑1515763                                                                                                                                         |                                                                                                                                     tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAg                                                                                                                                   >  1:1454007/1‑140 (MQ=255) tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAA‑CACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAagcagc                                                                                                                                  <  2:87260/140‑1 (MQ=255)  aacaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAagcagc                                                                                                                                  <  2:665433/140‑1 (MQ=255)  aacaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAagcagc                                                                                                                                  <  2:1322056/140‑1 (MQ=255)  aacaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAagcagc                                                                                                                                  <  2:1135182/140‑1 (MQ=255)   acaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGcc                                                                                                                                 <  1:105381/140‑1 (MQ=255)   acaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGcc                                                                                                                                 <  2:943766/140‑1 (MQ=255)   acaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGcc                                                                                                                                 <  2:148445/140‑1 (MQ=255)   acaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGcc                                                                                                                                 <  1:1418106/140‑1 (MQ=255)    caacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCt                                                                                                                                <  2:558939/140‑1 (MQ=255)    caacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCt                                                                                                                                <  2:716473/140‑1 (MQ=255)      acCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGt                                                                                                                              >  2:611808/1‑140 (MQ=255)                    gggggCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCaa                                                                                                                <  1:193183/140‑1 (MQ=255)                          cTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACggggg                                                                                                          >  1:256357/1‑140 (MQ=255)                           tggtggCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGa                                                                                                         >  2:1485042/1‑140 (MQ=255)                           tggtggCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGa                                                                                                         >  2:266817/1‑140 (MQ=255)                            ggtggCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGAc                                                                                                        >  1:1131769/1‑140 (MQ=255)                                      ccGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTgg                                                                                              <  2:656745/140‑1 (MQ=255)                                          gAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAAt                                                                                          >  1:285135/1‑140 (MQ=255)                                                gACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGa                                                                                    >  1:408871/1‑140 (MQ=255)                                                   aaTGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGc                                                                                 <  2:916543/140‑1 (MQ=255)                                                   aaTGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGc                                                                                 <  1:611808/140‑1 (MQ=255)                                                     tGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCAt                                                                               <  2:373848/140‑1 (MQ=255)                                                        tCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCg                                                                            >  1:1373528/1‑140 (MQ=255)                                                        tCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCg                                                                            <  1:632558/140‑1 (MQ=255)                                                        tCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCg                                                                            <  2:907002/140‑1 (MQ=255)                                                        tCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCg                                                                            >  2:940346/1‑140 (MQ=255)                                                             aaCCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCagag                                                                       <  2:168273/140‑1 (MQ=255)                                                               ccACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCa                                                                     >  1:42982/1‑140 (MQ=255)                                                                 aCTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAgg                                                                   >  2:687793/1‑140 (MQ=255)                                                                 aCTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAgg                                                                   >  1:1201400/1‑140 (MQ=255)                                                                  cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                                  >  1:598351/1‑140 (MQ=255)                                                                  cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                                  <  2:544547/140‑1 (MQ=255)                                                                  cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                                  <  1:804306/140‑1 (MQ=255)                                                                  cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                                  <  1:1451636/140‑1 (MQ=255)                                                                  cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                                  >  2:920760/1‑140 (MQ=255)                                                                           gAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGAtgctgc                                                         >  1:602659/1‑140 (MQ=255)                                                                             atatGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGctgctg                                                       >  1:1022348/1‑140 (MQ=255)                                                                                tgctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgc                                                    >  2:264710/1‑140 (MQ=255)                                                                                tgctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgc                                                    >  2:1428230/1‑140 (MQ=255)                                                                                 gctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgcg                                                   <  2:1013559/140‑1 (MQ=255)                                                                                 gctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgcg                                                   >  2:1108354/1‑140 (MQ=255)                                                                                 gctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgcg                                                   >  1:1333977/1‑140 (MQ=255)                                                                                   tatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGATAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgcgcg                                                 >  1:1441017/1‑140 (MQ=255)                                                                                     tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGcgcgcg                                               >  1:1407480/1‑140 (MQ=255)                                                                                     tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGcgcgcg                                               >  1:415160/1‑140 (MQ=255)                                                                                     tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGcgcgcg                                               >  2:1102188/1‑140 (MQ=255)                                                                                       cAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGc                                             <  1:650068/140‑1 (MQ=255)                                                                                        aaCATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCa                                            >  1:1058408/1‑140 (MQ=255)                                                                                          cATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGa                                          >  1:192609/1‑140 (MQ=255)                                                                                            tAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATg                                        >  1:377963/1‑140 (MQ=255)                                                                                            tAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATg                                        >  1:271135/1‑140 (MQ=255)                                                                                               gTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATgcag                                     <  2:408871/140‑1 (MQ=255)                                                                                                 tACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATgcagca                                   <  1:1174208/140‑1 (MQ=255)                                                                                                 tACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATgcagca                                   >  2:371284/1‑140 (MQ=255)                                                                                                      aTTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGc                              >  1:784243/1‑140 (MQ=255)                                                                                                      aTTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGc                              >  2:374220/1‑140 (MQ=255)                                                                                                        tAACGGAGAACTGGGTAGCGGCGGAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGCCGTTTTACTGGAAATTGCCGAGGCATCCGCAGGGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCg                            >  2:326581/1‑140 (MQ=255)                                                                                                          aCGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGaa                          <  2:602659/140‑1 (MQ=255)                                                                                                          aCGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGaa                          <  1:1215527/140‑1 (MQ=255)                                                                                                           cGGAGAACTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAAt                         <  1:979506/140‑1 (MQ=255)                                                                                                                aaCTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATggggg                    >  2:476712/1‑140 (MQ=255)                                                                                                                aaCTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATggggg                    >  1:63945/1‑140 (MQ=255)                                                                                                                 aCTGGTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGc                   <  1:1485042/140‑1 (MQ=255)                                                                                                                     gTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGACATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAc               <  2:1172914/140‑1 (MQ=255)                                                                                                                     gTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAc               >  2:1282450/1‑140 (MQ=255)                                                                                                                     gTTAGCGGCGAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAc               >  1:677578/1‑140 (MQ=255)                                                                                                                          cggcgAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGc          >  1:1290074/1‑140 (MQ=255)                                                                                                                            gcgAAGGGGAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCg        >  1:497834/1‑140 (MQ=255)                                                                                                                                 ggggAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAAAgt   <  2:469628/140‑1 (MQ=255)                                                                                                                                  gggAAAAGCAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAAAgtg  <  2:784243/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAAAGTG  >  NC_000913/1515495‑1515763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑