Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,873	T→C	intergenic (+72/‑61)	ydjM → / → tcyP	putative inner membrane protein regulated by LexA/cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,873	0	T	C	100.0%	234.3 / NA	62	intergenic (+72/‑61)	ydjM/tcyP	putative inner membrane protein regulated by LexA/cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/32);  total (30/32)

ATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCAT  >  NC_000913/1810742‑1811005                                                                                                                                    |                                                                                                                                     aTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGcc                                                                                                                              <  1:533238/140‑1 (MQ=255) aTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGcc                                                                                                                              <  1:989406/140‑1 (MQ=255) aTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGcc                                                                                                                              <  1:1487301/140‑1 (MQ=255) aTGATCAATACCTTGCAGATCCAGCTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGcc                                                                                                                              <  2:417709/140‑1 (MQ=255)         tACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTAt                                                                                                                      <  2:1489098/140‑1 (MQ=255)           ccTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGa                                                                                                                    <  1:136453/140‑1 (MQ=255)             ttGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACt                                                                                                                  >  2:231516/1‑140 (MQ=255)              tGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTg                                                                                                                 >  2:242504/1‑140 (MQ=255)              tGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTg                                                                                                                 >  2:524509/1‑140 (MQ=255)              tGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGACAAAAATCACCTTTCTGGAATATGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTg                                                                                                                 >  1:158840/1‑140 (MQ=255)                cAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCt                                                                                                               <  1:509876/140‑1 (MQ=255)                   aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACg                                                                                                            <  2:1334931/140‑1 (MQ=255)                   aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACg                                                                                                            >  1:818138/1‑140 (MQ=255)                   aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACg                                                                                                            <  1:1241007/140‑1 (MQ=255)                   aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACg                                                                                                            <  1:1113043/140‑1 (MQ=255)                   aTCCAGTGTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACg                                                                                                            <  2:1452034/140‑1 (MQ=255)                    tCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGc                                                                                                           <  1:625668/140‑1 (MQ=255)                     ccAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCa                                                                                                          >  1:1160315/1‑140 (MQ=255)                        gTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTg                                                                                                       >  2:305741/1‑140 (MQ=255)                         tttCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGa                                                                                                      >  2:1493543/1‑140 (MQ=255)                         tttCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGa                                                                                                      >  2:1278321/1‑140 (MQ=255)                              tCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGaa                                                                                                 >  2:489869/1‑140 (MQ=255)                                 gCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaata                                                                                              >  1:460927/1‑140 (MQ=255)                                   ttattaAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat                                                                                            <  1:231516/140‑1 (MQ=255)                                   ttattaAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAaataat                                                                                            <  2:1208149/140‑1 (MQ=255)                                        aaGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGAt                                                                                       <  2:1094129/140‑1 (MQ=255)                                                ggTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGaa                                                                               >  1:343608/1‑140 (MQ=255)                                                ggTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGaa                                                                               <  2:8441/140‑1 (MQ=255)                                                ggTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGaa                                                                               >  1:1253330/1‑140 (MQ=255)                                                ggTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGaa                                                                               >  2:562617/1‑140 (MQ=255)                                                  ttGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACg                                                                             <  2:499287/140‑1 (MQ=255)                                                    gAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACggg                                                                           >  1:1400225/1‑140 (MQ=255)                                                     aaTACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACgggg                                                                          <  2:818138/140‑1 (MQ=255)                                                        aCTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATg                                                                       <  2:609556/140‑1 (MQ=255)                                                           aaaaaGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAAc                                                                    <  2:1166371/140‑1 (MQ=255)                                                           aaaaaGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAAc                                                                    >  1:1306279/1‑140 (MQ=255)                                                               aGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTc                                                                >  2:960178/1‑140 (MQ=255)                                                                  cAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCAt                                                             >  1:619007/1‑140 (MQ=255)                                                                         cACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGc                                                      <  1:242504/140‑1 (MQ=255)                                                                           ccTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGa                                                    >  1:992546/1‑140 (MQ=255)                                                                           ccTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGa                                                    <  2:714223/140‑1 (MQ=255)                                                                              ttCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACa                                                 <  2:568691/140‑1 (MQ=255)                                                                                cTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATc                                               <  1:305741/140‑1 (MQ=255)                                                                                   gAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACCCTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtg                                            <  1:717679/140‑1 (MQ=255)                                                                                   gAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtg                                            <  1:627209/140‑1 (MQ=255)                                                                                   gAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtg                                            <  2:566695/140‑1 (MQ=255)                                                                                        aGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGtt                                       >  2:1017809/1‑140 (MQ=255)                                                                                        aGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGtt                                       <  2:930726/140‑1 (MQ=255)                                                                                        aGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGtt                                       >  1:1229816/1‑140 (MQ=255)                                                                                         gCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTc                                      >  1:617426/1‑140 (MQ=255)                                                                                          cAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCg                                     <  1:562617/140‑1 (MQ=255)                                                                                          cAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCg                                     <  1:1493543/140‑1 (MQ=255)                                                                                              tCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGt                                 >  1:1083083/1‑140 (MQ=255)                                                                                                  tttGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTActg                             >  2:500499/1‑140 (MQ=255)                                                                                                  tttGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTActg                             >  1:442705/1‑140 (MQ=255)                                                                                                  tttGAATATAAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTActg                             >  1:1388927/1‑140 (MQ=255)                                                                                                          taAGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTgc                     >  2:293713/1‑140 (MQ=255)                                                                                                           aaGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTgcg                    <  2:1306279/140‑1 (MQ=255)                                                                                                           aaGAGCCAGCTCACAGTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTgcg                    <  1:960178/140‑1 (MQ=255)                                                                                                                          gTTCTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGc     >  1:166646/1‑140 (MQ=255)                                                                                                                             cTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCAt  >  1:102208/1‑140 (MQ=255)                                                                                                                             cTGTTAACCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGAGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCAt  >  1:1352210/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     ATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCAT  >  NC_000913/1810742‑1811005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑