Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310,450	A→G	A74A (GCT→GCC)	ecpA ←	common pilus major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	310,450	0	A	G	100.0%	279.3 / NA	75	A74A (GCT→GCC)	ecpA	common pilus major subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/39);  total (36/39)

CAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGACCAGGTCGCT  >  NC_000913/310314‑310582                                                                                                                                         |                                                                                                                                     cAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGcc                                                                                                                                   <  2:542347/140‑1 (MQ=255)   gTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCAc                                                                                                                                 >  1:450353/1‑140 (MQ=255)    ttttttCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACg                                                                                                                                <  2:554099/140‑1 (MQ=255)    ttttttCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACg                                                                                                                                <  2:662656/140‑1 (MQ=255)        ttCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCaa                                                                                                                            <  1:1235510/140‑1 (MQ=255)          cGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAAt                                                                                                                          <  1:1160749/140‑1 (MQ=255)            aCTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAg                                                                                                                        <  2:1497308/140‑1 (MQ=255)             cTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATaaa                                                                                                                       <  2:118035/140‑3 (MQ=255)                   cgcCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTc                                                                                                                 >  1:628795/1‑140 (MQ=255)                      cGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCtgt                                                                                                              <  2:1066837/140‑1 (MQ=255)                                cACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAAc                                                                                                    >  1:179516/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCCCGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:480501/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:610670/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:80600/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:1131872/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGGCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:1471196/1‑140 (MQ=255)                                  cGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGCGTTGGCGATAAGACATGTGGTCAGTTTAAAGGCGGTAGCCGGTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCt                                                                                                  >  2:1313244/1‑140 (MQ=255)                                   gCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCtt                                                                                                 >  1:1369721/1‑140 (MQ=255)                                       acaTTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAAt                                                                                             <  1:1169669/140‑1 (MQ=255)                                       acaTTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAAt                                                                                             <  2:124258/140‑1 (MQ=255)                                         aTTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGc                                                                                           <  1:1462828/140‑1 (MQ=255)                                         aTTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGc                                                                                           <  1:1060617/140‑1 (MQ=255)                                          ttCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGcc                                                                                          <  2:692675/140‑1 (MQ=255)                                            cAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCtt                                                                                        <  2:1124084/140‑1 (MQ=255)                                            cAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCtt                                                                                        <  1:781770/140‑1 (MQ=255)                                            cAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCtt                                                                                        <  1:1030849/140‑1 (MQ=255)                                             aGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTc                                                                                       >  2:34040/1‑140 (MQ=255)                                             aGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTc                                                                                       >  1:104449/1‑140 (MQ=255)                                             aGTGTGGAACCTGAGGTATACAACTGGGTTAATGAGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGATGAGTCACCCTCGATTGCCACGTCAAATAGACCTTACTGTGAGTTAAAACCTTTAATGCCCTc                                                                                       >  2:241755/1‑140 (MQ=255)                                              gtgtGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCg                                                                                      <  2:628795/140‑1 (MQ=255)                                              gtgtGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCg                                                                                      <  2:258382/140‑1 (MQ=255)                                              gtgtGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCg                                                                                      <  2:1143057/140‑1 (MQ=255)                                              gtgtGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGAATAAAGGCGGTAGCTGTTGAGTCTCCCTCGATGGCGACGTCAAATAGACCTTTCTGTGAGTTAAATTCTTTAATGCCTTCg                                                                                      <  2:837932/140‑1 (MQ=255)                                               tgtgGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCgg                                                                                     >  2:625227/1‑140 (MQ=255)                                               tgtgGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCgg                                                                                     >  2:391956/1‑140 (MQ=255)                                               tgtgGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCgg                                                                                     >  2:174171/1‑140 (MQ=255)                                                    aaCCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATAc                                                                                >  1:1302605/1‑140 (MQ=255)                                                            gTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGc                                                                        <  2:856503/140‑1 (MQ=255)                                                             tATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGcc                                                                       <  1:897137/140‑1 (MQ=255)                                                             tATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGcc                                                                       <  2:964347/140‑1 (MQ=255)                                                               tCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAg                                                                     >  1:428752/1‑140 (MQ=255)                                                                    cTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTAc                                                                >  1:494431/1‑140 (MQ=255)                                                                     tGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCCCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTAcc                                                               <  1:1371996/140‑1 (MQ=255)                                                                      gggTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCg                                                              <  1:496307/140‑1 (MQ=255)                                                                         ttAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAgg                                                           >  2:748268/1‑139 (MQ=255)                                                                            aTGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGc                                                        >  1:784347/1‑140 (MQ=255)                                                                             tgtgTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCg                                                       >  2:443525/1‑140 (MQ=255)                                                                              gtgtTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGt                                                      <  1:706683/140‑1 (MQ=255)                                                                               tgtTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTc                                                     <  2:930673/140‑1 (MQ=255)                                                                                 ttGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTcac                                                   >  1:1491917/1‑140 (MQ=255)                                                                                 ttGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTcac                                                   >  2:352122/1‑140 (MQ=255)                                                                                 ttGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTcac                                                   >  2:918542/1‑140 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  2:102317/140‑1 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  2:1064688/140‑1 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  1:625227/140‑1 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  2:119559/140‑1 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  2:551844/140‑1 (MQ=255)                                                                                    gTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAAc                                                <  2:676650/140‑1 (MQ=255)                                                                                       aTAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAg                                             <  2:428752/140‑1 (MQ=255)                                                                                       aTAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAg                                             >  2:1292083/1‑140 (MQ=255)                                                                                         aaGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCt                                           <  1:666672/140‑1 (MQ=255)                                                                                           gACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTa                                         >  1:626057/1‑140 (MQ=255)                                                                                           gACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTa                                         >  2:173532/1‑140 (MQ=255)                                                                                            aCGCGAGGTCAGTTTAAAGGCGGTGGCCGCTGAGTCACCCTCGATTGCCATGTCAAATAGACCTTTCTGATAGTTTAAACATTTAATGGCTTCGGCCTACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTAc                                        >  2:826810/1‑140 (MQ=255)                                                                                              gTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACtg                                      >  2:792730/1‑140 (MQ=255)                                                                                              gTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACtg                                      >  2:632958/1‑140 (MQ=255)                                                                                               tGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACtgg                                     >  1:349448/1‑140 (MQ=255)                                                                                               tGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACtgg                                     >  1:515373/1‑140 (MQ=255)                                                                                                   gTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTggtggt                                 >  1:51568/1‑140 (MQ=255)                                                                                                    tCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGgtggtg                                <  2:1045429/140‑1 (MQ=255)                                                                                                          tAAAGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCtttt                          >  2:13910/1‑140 (MQ=255)                                                                                                             aGGCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTg                       >  2:277402/1‑140 (MQ=255)                                                                                                               gCGGTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGc                     <  1:34040/140‑1 (MQ=255)                                                                                                                  gTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGt                  >  2:635757/1‑140 (MQ=255)                                                                                                                  gTAGCCGTTGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGt                  >  2:1107662/1‑140 (MQ=255)                                                                                                                         ttGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGAc           <  1:174171/140‑1 (MQ=255)                                                                                                                         ttGAGTCACCCTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGAc           <  2:430233/140‑1 (MQ=255)                                                                                                                                 cccTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGACCAGGTCGc   >  2:1114123/1‑140 (MQ=255)                                                                                                                                  ccTCGATGGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGACCAGGTCGCt  <  1:480501/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTAAAACCTTTAATGCCTTCGGCATACTGGAACGCCAGGCTACCGAGTGGCGTCACAACCAGCTTACTGGTGGTGTCTTTTTTGGCTGTTGCCGACCAGGTCGCT  >  NC_000913/310314‑310582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑