Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,017	T→C	G41G (GGT→GGC)	manZ →	mannose‑specific PTS enzyme IID component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,017	0	T	C	100.0%	295.4 / NA	78	G41G (GGT→GGC)	manZ	mannose‑specific PTS enzyme IID component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/40);  total (38/40)

TAACAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACC  >  NC_000913/1903880‑1904152                                                                                                                                          |                                                                                                                                        tAACAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGctt                                                                                                                                       <  1:111061/140‑1 (MQ=255)  aaCAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTc                                                                                                                                      <  1:1415388/140‑1 (MQ=255)   aCAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCt                                                                                                                                     >  2:433216/1‑140 (MQ=255)   aCAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCt                                                                                                                                     <  2:334463/140‑1 (MQ=255)    cAGGTGAGCGAAATGGTTGATACAACTCAAAGTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTg                                                                                                                                    <  2:87918/140‑1 (MQ=255)    cAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTg                                                                                                                                    >  1:636090/1‑140 (MQ=255)     aGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTTCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGc                                                                                                                                   <  2:479422/140‑1 (MQ=255)     aGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGc                                                                                                                                   <  1:942672/140‑1 (MQ=255)       gTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCtt                                                                                                                                 <  1:891605/140‑1 (MQ=255)       gTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCtt                                                                                                                                 <  2:638041/140‑1 (MQ=255)       gTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCtt                                                                                                                                 <  1:1315193/140‑1 (MQ=255)         gAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGgcttctgcttct                                                                                                                               >  2:1469418/1‑140 (MQ=255)          aGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTtctc                                                                                                                              >  1:801005/1‑140 (MQ=255)           gCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTctct                                                                                                                             <  1:710545/140‑1 (MQ=255)           gCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTctct                                                                                                                             <  2:1498330/140‑1 (MQ=255)                           aaCTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATtc                                                                                                             <  2:501089/140‑1 (MQ=255)                             cTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATtcgt                                                                                                           <  1:740254/140‑1 (MQ=255)                             cTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATtcgt                                                                                                           >  1:667775/1‑140 (MQ=255)                             cTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATtcgt                                                                                                           >  1:355136/1‑140 (MQ=255)                              tCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATtcgtc                                                                                                          >  2:55084/1‑140 (MQ=255)                                 aaCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGcc                                                                                                       <  2:802855/140‑1 (MQ=255)                                  aCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCct                                                                                                      >  1:1457561/1‑140 (MQ=255)                                  aCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCct                                                                                                      <  1:392331/140‑1 (MQ=255)                                       caccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAcc                                                                                                 >  2:150419/1‑140 (MQ=255)                                        accGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAccc                                                                                                <  2:1383538/140‑1 (MQ=255)                                            agaAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTgag                                                                                            >  1:646828/1‑140 (MQ=255)                                            agaAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTgag                                                                                            >  2:390738/1‑140 (MQ=255)                                                    ctcactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGa                                                                                    <  1:433216/140‑1 (MQ=255)                                                    ctcactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGa                                                                                    <  2:1275535/140‑1 (MQ=255)                                                       actcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGc                                                                                 >  1:135498/1‑140 (MQ=255)                                                               tGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAAc                                                                         <  1:61526/140‑1 (MQ=255)                                                                       gTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTAtt                                                                 >  2:1471562/1‑140 (MQ=255)                                                                         ggCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCg                                                               <  2:564624/140‑1 (MQ=255)                                                                         ggCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCg                                                               >  2:828933/1‑140 (MQ=255)                                                                         ggCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCg                                                               <  2:1125381/140‑1 (MQ=255)                                                                           cGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGcc                                                             <  2:1498326/140‑1 (MQ=255)                                                                           cGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGcc                                                             >  2:1220196/1‑140 (MQ=255)                                                                           cGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGcc                                                             <  2:899362/140‑1 (MQ=255)                                                                               ttCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCa                                                         >  2:111513/1‑140 (MQ=255)                                                                                 ccTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCAcc                                                       <  2:135498/140‑1 (MQ=255)                                                                                   tGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                     <  1:477686/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  2:289483/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  1:1485750/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  2:420421/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  2:355136/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  1:808277/140‑1 (MQ=255)                                                                                      gTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAg                                                  <  1:828933/140‑1 (MQ=255)                                                                                       ttCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGt                                                 >  1:1365348/1‑140 (MQ=255)                                                                                        tCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGtt                                                <  1:150419/140‑1 (MQ=255)                                                                                         cTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTc                                               >  1:697300/1‑140 (MQ=255)                                                                                            aCCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCttt                                            <  1:228816/140‑1 (MQ=255)                                                                                                 ttCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAGACAAGCTATTCGCCGTCACCTGGAGTTCTTTAacac                                       >  1:497619/1‑140 (MQ=255)                                                                                                 ttCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCGCCTGGAGTTCTTTAacac                                       >  2:396914/1‑140 (MQ=255)                                                                                                 ttCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAacac                                       <  2:861007/140‑1 (MQ=255)                                                                                                  tCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACAcc                                      >  2:496296/1‑140 (MQ=255)                                                                                                  tCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACAcc                                      >  2:35272/1‑140 (MQ=255)                                                                                                    cAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCa                                    <  1:913852/140‑1 (MQ=255)                                                                                                     aGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAg                                   <  2:636090/140‑1 (MQ=255)                                                                                                     aGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAg                                   <  1:55084/140‑1 (MQ=255)                                                                                                      gggTTCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGc                                  <  1:236944/140‑1 (MQ=255)                                                                                                         ttCATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGt                               >  1:224906/1‑140 (MQ=255)                                                                                                           cATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                             >  1:467558/1‑140 (MQ=255)                                                                                                           cATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                             >  1:1149757/1‑140 (MQ=255)                                                                                                           cATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                             >  1:667550/1‑140 (MQ=255)                                                                                                           cATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGAACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                             >  1:507424/1‑140 (MQ=255)                                                                                                            aTGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGGCGTTCg                            >  2:1086655/1‑140 (MQ=255)                                                                                                             tGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGt                           >  2:1000850/1‑140 (MQ=255)                                                                                                              ggAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTg                          >  1:284173/1‑140 (MQ=255)                                                                                                              ggAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTg                          <  1:235612/140‑1 (MQ=255)                                                                                                                   ttCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTgc                     >  1:1474849/1‑140 (MQ=255)                                                                                                                            aTGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtct            >  1:418758/1‑140 (MQ=255)                                                                                                                            aTGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtct            >  1:1011859/1‑140 (MQ=255)                                                                                                                             tGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtctc           >  2:268280/1‑140 (MQ=255)                                                                                                                             tGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATtctc           >  1:1242378/1‑140 (MQ=255)                                                                                                                               cAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCgg         <  1:1469418/140‑1 (MQ=255)                                                                                                                                 ggCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCg       <  2:364185/140‑1 (MQ=255)                                                                                                                                    aCTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTaa    >  1:239622/1‑140 (MQ=255)                                                                                                                                     cTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAc   >  2:1400945/1‑140 (MQ=255)                                                                                                                                     cTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAc   >  2:1184171/1‑140 (MQ=255)                                                                                                                                      tGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAcc  >  1:881565/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TAACAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACC  >  NC_000913/1903880‑1904152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑