Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	349,031	A→G	Q117R (CAG→CGG)	prpB →	2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	349,031	0	A	G	100.0%	255.5 / NA	69	Q117R (CAG→CGG)	prpB	2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/39);  total (30/39)

TCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTC  >  NC_000913/348894‑349165                                                                                                                                          |                                                                                                                                       tCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCggg                                                                                                                                      >  2:447239/1‑140 (MQ=255)        aTCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGc                                                                                                                               >  2:355961/1‑140 (MQ=255)         tCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCg                                                                                                                              >  2:1355861/1‑140 (MQ=255)           aCCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCGTATTGAAGATCGGGTTGGTGCGaa                                                                                                                            >  2:871100/1‑140 (MQ=255)            ccGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGaaa                                                                                                                           <  2:767665/140‑1 (MQ=255)                    tGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGAGAAATCGATTATTAAAGCCGGTGCGGCAGGTTTGTATATTGAAGATCGGGTTGGTGCGAAACGCTGCgg                                                                                                                   >  2:1442733/1‑140 (MQ=255)                          ctgccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCg                                                                                                             <  1:912676/140‑1 (MQ=255)                           tgccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGt                                                                                                            <  1:70037/140‑1 (MQ=255)                           tgccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGt                                                                                                            >  2:802161/1‑140 (MQ=255)                                     ggtggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAg                                                                                                  >  2:908989/1‑140 (MQ=255)                                       tggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCg                                                                                                >  2:549634/1‑140 (MQ=255)                                        ggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGa                                                                                               <  1:1283408/140‑1 (MQ=255)                                        ggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGa                                                                                               >  1:90946/1‑140 (MQ=255)                                        ggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGa                                                                                               <  1:616546/140‑1 (MQ=255)                                         gATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGAt                                                                                              <  2:1260649/140‑1 (MQ=255)                                         gATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGAt                                                                                              <  1:588841/140‑1 (MQ=255)                                         gATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACACTGCGGTCATCGTCCGAATAAAGCGAt                                                                                              <  2:645628/140‑1 (MQ=255)                                           tGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCg                                                                                            >  1:835310/1‑140 (MQ=255)                                            gCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGt                                                                                           <  2:1179302/140‑1 (MQ=255)                                            gCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGt                                                                                           <  2:881507/140‑1 (MQ=255)                                             cGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGtc                                                                                          >  1:495266/1‑140 (MQ=255)                                              ggATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGtct                                                                                         <  1:502250/140‑1 (MQ=255)                                              ggATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGtct                                                                                         <  1:1497166/140‑1 (MQ=255)                                                  atCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGaa                                                                                     <  1:481012/140‑1 (MQ=255)                                                    cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAaag                                                                                   <  2:242194/140‑1 (MQ=255)                                                       ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAaagaag                                                                                <  1:1495014/140‑1 (MQ=255)                                                       ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAaagaag                                                                                <  1:324957/140‑1 (MQ=255)                                                       ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAaagaag                                                                                <  1:1070340/140‑1 (MQ=255)                                                          tGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAgaga                                                                             >  2:17017/1‑140 (MQ=255)                                                           ggTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAt                                                                            <  1:355961/140‑1 (MQ=255)                                                             ttcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAtgg                                                                          <  1:1355788/140‑1 (MQ=255)                                                                 tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTgga                                                                      <  1:993817/140‑1 (MQ=255)                                                                       tttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatcggat                                                                <  1:516184/140‑1 (MQ=255)                                                                         tAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATcc                                                              >  1:296055/1‑140 (MQ=255)                                                                          aaCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcg                                                             <  2:884668/140‑1 (MQ=255)                                                                          aaCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcg                                                             >  2:94930/1‑140 (MQ=255)                                                                            cGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcg                                                           <  1:447239/140‑1 (MQ=255)                                                                             gTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgc                                                          <  1:871100/140‑1 (MQ=255)                                                                             gTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgc                                                          <  2:341139/140‑1 (MQ=255)                                                                               ggCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGGTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGGCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcgg                                                        >  2:166205/1‑140 (MQ=255)                                                                                gcgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcggc                                                       >  2:946711/1‑140 (MQ=255)                                                                                gcgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcggc                                                       >  2:269123/1‑140 (MQ=255)                                                                                gcgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcggc                                                       >  2:937244/1‑140 (MQ=255)                                                                                   cgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGcggcggg                                                    >  2:1117843/1‑139 (MQ=255)                                                                                        cGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATg                                               >  2:1436642/1‑140 (MQ=255)                                                                                          tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCg                                             <  2:456113/140‑1 (MQ=255)                                                                                                  atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGa                                     >  2:719578/1‑140 (MQ=255)                                                                                                  atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGa                                     >  1:1169134/1‑140 (MQ=255)                                                                                                    gatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATc                                   >  2:1010807/1‑140 (MQ=255)                                                                                                     atTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATcc                                  <  1:937970/140‑1 (MQ=255)                                                                                                      ttAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCt                                 <  1:15910/140‑1 (MQ=255)                                                                                                       tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTg                                <  1:802161/140‑1 (MQ=255)                                                                                                        aaaGCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGa                               >  2:1410821/1‑140 (MQ=255)                                                                                                           gCCGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                            <  2:495266/140‑1 (MQ=255)                                                                                                             cGGTGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                          >  1:301185/1‑140 (MQ=255)                                                                                                                tGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGa                       >  2:165273/1‑140 (MQ=255)                                                                                                                tGCGGCAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGa                       >  1:1104659/1‑140 (MQ=255)                                                                                                                     cAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATg                  <  2:296055/140‑1 (MQ=255)                                                                                                                     cAGGATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATg                  <  1:549634/140‑1 (MQ=255)                                                                                                                       ggATTGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGAGATCATGgc                >  1:882336/1‑140 (MQ=255)                                                                                                                          ttGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcgcg             <  1:908989/140‑1 (MQ=255)                                                                                                                          ttGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcgcg             >  1:62119/1‑140 (MQ=255)                                                                                                                          ttGCATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcgcg             <  1:1010807/140‑1 (MQ=255)                                                                                                                             cATATTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAc          >  1:1031253/1‑140 (MQ=255)                                                                                                                              atatTGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAcc         >  2:740226/1‑140 (MQ=255)                                                                                                                                  tGAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATg     >  2:991474/1‑140 (MQ=255)                                                                                                                                   gAAGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGc    <  1:1442733/140‑1 (MQ=255)                                                                                                                                    aaGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct   >  2:478105/1‑140 (MQ=255)                                                                                                                                    aaGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct   >  1:276478/1‑140 (MQ=255)                                                                                                                                    aaGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct   <  1:269123/140‑1 (MQ=255)                                                                                                                                    aaGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct   <  1:166205/140‑1 (MQ=255)                                                                                                                                     aGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGctc  <  2:1350378/140‑1 (MQ=255)                                                                                                                                     aGATCGGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGctc  <  2:1031719/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTC  >  NC_000913/348894‑349165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑