Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,002,309	T→C	N433S (AAC→AGC)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,002,309	0	T	C	100.0%	323.8 / NA	86	N433S (AAC→AGC)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/34);  total (52/34)

CGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGTTTGCA  >  NC_000913/2002175‑2002444                                                                                                                                       |                                                                                                                                        cGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTgc                                                                                                                                    >  2:565012/1‑140 (MQ=255)   gCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTgcac                                                                                                                                  <  1:1255324/140‑1 (MQ=255)     ctgctgGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTgcaccg                                                                                                                                >  2:1045710/1‑140 (MQ=255)                  tGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGgaaga                                                                                                                   >  2:1463555/1‑140 (MQ=255)                   gcgcTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGgaagaa                                                                                                                  >  2:990189/1‑140 (MQ=255)                   gcgcTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGgaagaa                                                                                                                  >  2:265165/1‑140 (MQ=255)                     gcTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGATTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACg                                                                                                                >  2:679640/1‑140 (MQ=255)                      cTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACgg                                                                                                               <  1:1448002/140‑1 (MQ=255)                         tCGACATATTGGCCCATTCGGTTGGCTCGTCGGCGTCCTGAATACGGGTCTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAAt                                                                                                            <  2:102179/140‑1 (MQ=255)                         tCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAAt                                                                                                            <  2:61692/140‑1 (MQ=255)                         tCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAAt                                                                                                            <  1:29701/140‑1 (MQ=255)                           gACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAAttt                                                                                                          <  1:1441525/140‑1 (MQ=255)                               tatTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCt                                                                                                      <  1:325898/140‑1 (MQ=255)                                   ggACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAg                                                                                                  >  2:1115219/1‑140 (MQ=255)                                    gACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGa                                                                                                 <  1:1458927/140‑1 (MQ=255)                                    gACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGa                                                                                                 <  2:237473/140‑1 (MQ=255)                                    gACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGa                                                                                                 <  1:1125415/140‑1 (MQ=255)                                        cTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCg                                                                                             <  1:544078/140‑1 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            <  2:1144663/140‑1 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            <  2:1062614/140‑1 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            >  2:566119/1‑140 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            >  2:593270/1‑140 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            <  2:75602/140‑1 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            >  1:618078/1‑140 (MQ=255)                                         ttCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGCAGCGGAGTTGATCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGa                                                                                            >  2:915344/1‑140 (MQ=255)                                           cGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATa                                                                                          >  1:929412/1‑140 (MQ=255)                                           cGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATa                                                                                          >  2:1009299/1‑140 (MQ=255)                                           cGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATa                                                                                          >  1:1189419/1‑140 (MQ=255)                                            ggTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAg                                                                                         >  2:1358286/1‑140 (MQ=255)                                              tCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCa                                                                                       >  2:1137266/1‑140 (MQ=255)                                              tCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCa                                                                                       >  2:539047/1‑140 (MQ=255)                                                 cATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCg                                                                                    >  2:1368266/1‑140 (MQ=255)                                                 cATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCg                                                                                    >  2:1477129/1‑140 (MQ=255)                                                   tAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTc                                                                                  >  1:588006/1‑140 (MQ=255)                                                      tCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAg                                                                               >  1:442254/1‑140 (MQ=255)                                                               tGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAg                                                                      <  2:322528/140‑1 (MQ=255)                                                                 aaTACGGGATTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCg                                                                    <  1:679640/140‑1 (MQ=255)                                                                     cGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATc                                                                <  2:781413/140‑1 (MQ=255)                                                                      gggACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATcc                                                               >  1:205414/1‑140 (MQ=255)                                                                      gggACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATcc                                                               >  2:1244981/1‑140 (MQ=255)                                                                       ggACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCg                                                              <  2:1458683/140‑1 (MQ=255)                                                                       ggACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCg                                                              >  2:841548/1‑140 (MQ=255)                                                                        gACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGt                                                             >  2:418924/1‑140 (MQ=255)                                                                           tGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGt                                                          >  1:1161984/1‑140 (MQ=255)                                                                           tGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGt                                                          >  2:1338462/1‑140 (MQ=255)                                                                             cgcTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGttt                                                        >  1:1015440/1‑140 (MQ=255)                                                                               cTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTa                                                      >  2:774897/1‑140 (MQ=255)                                                                                  cAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTTCAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCa                                                   <  1:71948/140‑1 (MQ=255)                                                                                  cAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCa                                                   <  2:607437/140‑1 (MQ=255)                                                                                         gTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCaa                                            >  1:1440252/1‑140 (MQ=255)                                                                                           tGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAAca                                          <  2:340536/140‑1 (MQ=255)                                                                                            ggTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAAcag                                         <  1:1045710/140‑1 (MQ=255)                                                                                            ggTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAAcag                                         <  1:1009299/140‑1 (MQ=255)                                                                                            ggTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAAcag                                         <  2:662249/140‑1 (MQ=255)                                                                                            ggTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAAcag                                         >  1:694251/1‑140 (MQ=255)                                                                                                 tggtgTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTc                                    >  2:624554/1‑140 (MQ=255)                                                                                                 tggtgTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTc                                    >  1:744687/1‑140 (MQ=255)                                                                                                   gtgtTGTTCAGGTTGGTAATCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCag                                  >  1:452053/1‑140 (MQ=255)                                                                                                   gtgtTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCag                                  >  1:273719/1‑140 (MQ=255)                                                                                                   gtgtTGTTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCag                                  >  2:916491/1‑140 (MQ=255)                                                                                                   ctgttgtTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCag                                  >  2:1194276/2‑140 (MQ=255)                                                                                                    tgttgtTCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCaga                                 <  1:990189/140‑1 (MQ=255)                                                                                                       tgttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGcc                              <  2:730776/140‑1 (MQ=255)                                                                                                       tgttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGcc                              <  2:929412/140‑1 (MQ=255)                                                                                                       tgttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGcc                              >  1:177693/1‑140 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCaa                                            >  2:424140/1‑125 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCaa                                            <  1:424140/125‑1 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             >  2:372913/1‑140 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             <  1:221350/140‑1 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             >  1:1356616/1‑140 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             >  1:1358149/1‑140 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             >  1:755987/1‑140 (MQ=255)                                                                                                        gttCAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCt                             >  2:415154/1‑140 (MQ=255)                                                                                                        gttCAGGTCGGCAACCGCGGAATCCAGACGGCTTTGCACCGCACCAAGGGAAGAACGGAATCTGTCTACAGATGCGCTAGCATCGTCCGGCGCTTTCAGCGGCTCCGTGGTTTTACCTTTTGCAACAGCAGTCAGAGCCt                             >  2:810588/1‑140 (MQ=255)                                                                                                           cAGGTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTcac                          >  1:447060/1‑140 (MQ=255)                                                                                                              gTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTcaccac                       >  1:1484500/1‑140 (MQ=255)                                                                                                              gTTGGTAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTcaccac                       >  1:639965/1‑140 (MQ=255)                                                                                                                   tAACCGCGGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACcagca                  >  2:1246689/1‑140 (MQ=255)                                                                                                                        gcgGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAGCAGCAGTCAGAGCCTCACCACCAGCAGTCaa             >  2:1150897/1‑140 (MQ=255)                                                                                                                        gcgGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCaa             >  2:1212444/1‑140 (MQ=255)                                                                                                                         cgGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCaaa            <  2:694251/140‑1 (MQ=255)                                                                                                                         cgGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCaaa            >  2:282744/1‑140 (MQ=255)                                                                                                                         cgGAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCaaa            >  2:106330/1‑140 (MQ=255)                                                                                                                          ggAATCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAAc           <  1:841548/140‑1 (MQ=255)                                                                                                                              tCCAGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGt       <  2:478367/140‑1 (MQ=255)                                                                                                                                 aGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGTTTg    <  2:442254/140‑1 (MQ=255)                                                                                                                                 aGACGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGTTTg    <  2:1356616/140‑1 (MQ=255)                                                                                                                                   aCGGCTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGTTTGCa  >  1:1472355/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAGGGAAGAACGGAATTTGTCTACAGATGCGATAGCATCGTCCAGCGCTTTCAGCGGATCCGTGGTTTTACCATTTGCAACAGCAGTCAGAGCCTCACCACCAGCAGTCAAACCTGTTTGCA  >  NC_000913/2002175‑2002444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑