Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,030,309	T→C	G257G (GGT→GGC)	yedA →	putative transporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,030,309	0	T	C	100.0%	221.5 / NA	59	G257G (GGT→GGC)	yedA	putative transporter YedA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/25);  total (34/25)

TTGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGTACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAGTTATTCAGGA  >  NC_000913/2030176‑2030443                                                                                                                                      |                                                                                                                                       ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  1:1020617/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  1:63706/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  2:1277251/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  2:1318790/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  2:1448150/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  2:262201/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGa                                                                                                                                  <  1:107667/140‑1 (MQ=255) ttGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGATCTTGCTGGGCACGGGa                                                                                                                                  <  2:797525/140‑1 (MQ=255)    cGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTg                                                                                                                               <  2:907618/140‑1 (MQ=255)      gTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTggg                                                                                                                             <  2:1490592/140‑1 (MQ=255)         ggCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTgg                                                                                                                          >  2:1370233/1‑140 (MQ=255)         ggCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTgg                                                                                                                          >  1:485927/1‑140 (MQ=255)                tGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAaca                                                                                                                   <  1:100759/140‑1 (MQ=255)                tGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAaca                                                                                                                   <  1:522806/140‑1 (MQ=255)                   cgcTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTg                                                                                                                >  2:328624/1‑140 (MQ=255)                        tttGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGaa                                                                                                           >  1:1493714/1‑140 (MQ=255)                             ttCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTg                                                                                                      >  2:1157715/1‑140 (MQ=255)                                gATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAAt                                                                                                   >  2:1026158/1‑140 (MQ=255)                                  ttATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAAtgg                                                                                                 >  2:964786/1‑140 (MQ=255)                                  ttATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAAtgg                                                                                                 >  1:1421196/1‑140 (MQ=255)                                  ttATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTACTGGGCACGGGACTGGGCGGAGAAACACTGTCGAAGATTGAAtgg                                                                                                 >  1:752955/1‑140 (MQ=255)                                          aTCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCt                                                                                         <  1:341395/140‑1 (MQ=255)                                          aTCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCt                                                                                         <  1:66638/140‑1 (MQ=255)                                           tCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCACTc                                                                                        >  1:569396/1‑140 (MQ=255)                                                          aTTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTc                                                                         <  1:886586/140‑1 (MQ=255)                                                             tAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTcgcg                                                                      <  1:760719/140‑1 (MQ=255)                                                              aaTCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCgg                                                                     <  1:1347163/140‑1 (MQ=255)                                                              aaTCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCgg                                                                     >  2:963067/1‑140 (MQ=255)                                                              aaTCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCgg                                                                     >  2:1245663/1‑140 (MQ=255)                                                                tCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCggtg                                                                   >  2:1376754/1‑140 (MQ=255)                                                                  cGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCggtggt                                                                 <  1:1370233/140‑1 (MQ=255)                                                                   gTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTa                                                                >  1:454415/1‑140 (MQ=255)                                                                   gTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTa                                                                >  1:983856/1‑140 (MQ=255)                                                                   gTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTa                                                                >  1:292570/1‑140 (MQ=255)                                                                     aaTGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACt                                                              >  1:160438/1‑140 (MQ=255)                                                                            gTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCCCGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACg                                                       >  2:1379946/1‑140 (MQ=255)                                                                            gTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACg                                                       >  2:1103370/1‑140 (MQ=255)                                                                            gTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACg                                                       >  2:1126895/1‑140 (MQ=255)                                                                             tCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGt                                                      >  2:36012/1‑140 (MQ=255)                                                                               cGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTg                                                    >  1:1025519/1‑140 (MQ=255)                                                                                 gCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTggg                                                  >  1:251222/1‑140 (MQ=255)                                                                                 gCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTggg                                                  >  2:835073/1‑140 (MQ=255)                                                                                  ctctCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGa                                                 >  2:1080371/1‑140 (MQ=255)                                                                                   tctcGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGaa                                                <  2:1175526/140‑1 (MQ=255)                                                                                        ccaccaGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAatat                                           <  2:1321008/140‑1 (MQ=255)                                                                                            caGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATctct                                       >  2:1306511/1‑140 (MQ=255)                                                                                                 aCGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCg                                  >  1:1398554/1‑140 (MQ=255)                                                                                                    cTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCa                               <  1:517222/140‑1 (MQ=255)                                                                                                        cGTTAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAAc                           >  2:350781/1‑140 (MQ=255)                                                                                                          ttAACCCGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAAccc                         <  1:1379946/140‑1 (MQ=255)                                                                                                              cccGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAg                     >  2:346152/1‑140 (MQ=255)                                                                                                               ccGGTGGTCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGt                    >  2:1021193/1‑140 (MQ=255)                                                                                                                 ggtggtCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTg                  >  1:519323/1‑140 (MQ=255)                                                                                                                  gtggtCGCGGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTgc                 <  2:1493714/140‑1 (MQ=255)                                                                                                                       tgcgGTCTTGCTGGGCGCGGGACTGGGGGGAGAAACCCTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTGCTGGTCACGTTGGGAAAATATCTGTTCCCGGCAAAACCCGTTGTTGCGCCAg            <  2:1328918/139‑1 (MQ=255)                                                                                                                       cgcgGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAg            >  1:1282899/1‑140 (MQ=255)                                                                                                                       cgcgGTCTTGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAg            <  1:963067/140‑1 (MQ=255)                                                                                                                               tGCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAGTTATTCAg    >  1:154408/1‑140 (MQ=255)                                                                                                                                gCTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAGTTATTCAgg   >  1:1265233/1‑140 (MQ=255)                                                                                                                                 cTGGGCACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAGTTATTCAGGa  <  1:1245663/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTGCGGTCGGCTATCTGGCGCTGTTTGGTTCGATTATCGCCATCAACGCTTATATGTATTTAATCCGTAATGTCAGTCCGGCTCTCGCCACCAGCTACGCTTACGTTAACCCGGTGGTCGCGGTCTTGCTGGGTACGGGACTGGGTGGAGAAACACTGTCGAAGATTGAATGGCTGGCGCTCGGCGTAATTGTCTTCGCGGTGGTACTGGTCACGTTGGGAAAATATCTCTTCCCGGCAAAACCCGTAGTTGCGCCAGTTATTCAGGA  >  NC_000913/2030176‑2030443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑