Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,942	T→C	K180K (AAA→AAG)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,942	0	T	C	100.0%	189.6 / NA	51	K180K (AAA→AAG)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/29);  total (22/29)

ACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGA  >  NC_000913/2218808‑2219077                                                                                                                                       |                                                                                                                                        aCCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTc                                                                                                                                    >  2:597137/1‑140 (MQ=255)                     aGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCgg                                                                                                                <  1:155407/140‑1 (MQ=255)                        cATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGgcgc                                                                                                             >  1:35980/1‑140 (MQ=255)                        cATGGCAGCTTTAACGCCAGAGGTTTGCGGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGgcgc                                                                                                             <  1:1332853/140‑1 (MQ=255)                         aTTGCAGCATTACCGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGcgcg                                                                                                            <  2:1378009/140‑1 (MQ=255)                         aTTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGcgcg                                                                                                            <  2:728012/140‑1 (MQ=255)                          ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           <  2:1168206/140‑1 (MQ=255)                          ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           >  1:910660/1‑140 (MQ=255)                          ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           >  1:161015/1‑140 (MQ=255)                                     aCGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAActc                                                                                                >  1:46553/1‑140 (MQ=255)                                       gCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTg                                                                                              <  2:1349248/140‑1 (MQ=255)                                          agagGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCg                                                                                           >  2:1349704/1‑140 (MQ=255)                                            agGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAg                                                                                         >  1:1448037/1‑140 (MQ=255)                                                 tGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGc                                                                                    >  1:1495431/1‑140 (MQ=255)                                                            ggCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAgg                                                                         <  2:574424/140‑1 (MQ=255)                                                             gCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGt                                                                        >  2:784644/1‑140 (MQ=255)                                                              cTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTg                                                                       <  2:264657/140‑1 (MQ=255)                                                                ttGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgcc                                                                     >  1:949494/1‑140 (MQ=255)                                                                 tgttCGTCACCGCCGTGTCGCCCCCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccg                                                                    <  2:861894/137‑1 (MQ=255)                                                                   agcGTCACCGAAGTGTCGCCTCCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATCGGCCTTTTCAAACTCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGGgccgcc                                                                  <  2:1293169/138‑1 (MQ=255)                                                                   aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:46553/140‑1 (MQ=255)                                                                   aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:582698/140‑1 (MQ=255)                                                                   aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:34082/140‑1 (MQ=255)                                                                   aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  1:288482/140‑1 (MQ=255)                                                                   aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  1:1198892/140‑1 (MQ=255)                                                                      gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  1:597137/140‑1 (MQ=255)                                                                      gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  2:1448037/140‑1 (MQ=255)                                                                      gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  2:647413/140‑1 (MQ=255)                                                                         aCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTg                                                            <  1:384631/140‑1 (MQ=255)                                                                           cgccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCa                                                          >  1:895150/1‑140 (MQ=255)                                                                           cgccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCa                                                          <  2:508980/140‑1 (MQ=255)                                                                             ccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGa                                                        >  2:1139370/1‑140 (MQ=255)                                                                                  tCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACg                                                   >  2:387355/1‑140 (MQ=255)                                                                                  tCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACg                                                   >  1:633631/1‑140 (MQ=255)                                                                                   cGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGa                                                  <  1:379311/140‑1 (MQ=255)                                                                                     ccaccaGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACt                                                <  1:955865/140‑1 (MQ=255)                                                                                       accaGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCa                                              >  1:489393/1‑140 (MQ=255)                                                                                          agccagTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGt                                           >  2:1486549/1‑140 (MQ=255)                                                                                             cagTGACAGCAACTGATCCTGACCGAACTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCgg                                        <  2:329389/140‑1 (MQ=255)                                                                                                    aGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGa                                 >  1:420049/1‑140 (MQ=255)                                                                                                      cAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAg                               >  1:149175/1‑140 (MQ=255)                                                                                                      cAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAg                               >  2:622358/1‑140 (MQ=255)                                                                                                              ccTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTa                       >  2:127856/1‑140 (MQ=255)                                                                                                                 gACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTAttt                    >  1:985734/1‑140 (MQ=255)                                                                                                                     gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  1:45039/140‑1 (MQ=255)                                                                                                                     gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:1495431/140‑1 (MQ=255)                                                                                                                     gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:1006407/140‑1 (MQ=255)                                                                                                                     gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:128080/140‑1 (MQ=255)                                                                                                                     gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  1:1349704/140‑1 (MQ=255)                                                                                                                          tAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGcc           <  1:1486549/140‑1 (MQ=255)                                                                                                                                 aTAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCt    >  1:758706/1‑140 (MQ=255)                                                                                                                                   aGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGa  <  2:910660/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGA  >  NC_000913/2218808‑2219077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑