Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	349,192	A→G	I171V (ATC→GTC)	prpB →	2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	349,192	0	A	G	100.0%	307.8 / NA	82	I171V (ATC→GTC)	prpB	2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/38);  total (44/38)

TCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTG  >  NC_000913/349059‑349327                                                                                                                                      |                                                                                                                                         tCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGc                                                                                                                                    <  2:882336/140‑1 (MQ=255)     aaTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgc                                                                                                                                >  1:1032791/1‑140 (MQ=255)     aaTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgc                                                                                                                                >  1:426463/1‑140 (MQ=255)      aTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgcg                                                                                                                               <  1:740226/140‑1 (MQ=255)      aTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgcg                                                                                                                               <  1:1410821/140‑1 (MQ=255)      aTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgcg                                                                                                                               <  2:634628/140‑1 (MQ=255)      aTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgcg                                                                                                                               <  2:1196576/140‑1 (MQ=255)      aTAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTgcg                                                                                                                               <  1:946711/140‑1 (MQ=255)           gCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGc                                                                                                                          <  1:1355861/140‑1 (MQ=255)            cGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGcc                                                                                                                         >  2:944705/1‑140 (MQ=255)            cGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGcc                                                                                                                         <  2:1031253/140‑1 (MQ=255)             gATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCt                                                                                                                        <  2:1104659/140‑1 (MQ=255)                cGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGGGCGTGCGCAGGCCTATg                                                                                                                     >  2:635873/1‑140 (MQ=255)                cGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATg                                                                                                                     >  1:984752/1‑140 (MQ=255)                         agaagaGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAAGCCTCTTTTTAAGCgg                                                                                                            >  2:948891/1‑140 (MQ=255)                            agagATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGGCGGGCTTTGTTGAAGCggggg                                                                                                         >  2:1374302/1‑138 (MQ=255)                             gagaTGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGGGGTCCCAGGGCCATGTTGTAGCGGGTGc                                                                                                        >  2:1099690/1‑140 (MQ=255)                              agaTGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGcc                                                                                                       >  1:295644/1‑140 (MQ=255)                               gaTGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCg                                                                                                      <  2:301185/140‑1 (MQ=255)                                 tggtggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGGTTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGGCGAGAGGGCGCAGGCCCATGTTGCAACGGGGGCCgcg                                                                                                    >  2:1481735/1‑138 (MQ=255)                                 tggtggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag                                                                                                    <  1:165273/140‑1 (MQ=255)                                 tggtggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag                                                                                                    <  1:17017/140‑1 (MQ=255)                                  ggtggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgaga                                                                                                   >  2:30167/1‑140 (MQ=255)                                    tggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAtg                                                                                                 <  2:1415007/140‑1 (MQ=255)                                    tggATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAtg                                                                                                 >  1:44015/1‑140 (MQ=255)                                     ggatcggatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCCGGCCTATGTTGAAGCGGGGGCCGGGAtgg                                                                                                >  2:1460826/1‑139 (MQ=255)                                        tcggatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCGGGCCTATGGTGAAGCGGGTGCCGAGAtgttgt                                                                                             >  1:1272156/1‑140 (MQ=255)                                        tcggatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCCGGCCTATGTTGAAGGGGGTGCCGGGAtgttgt                                                                                             >  2:263325/1‑140 (MQ=255)                                          ggatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGGGCGTGCGCAGGCCTCTGTTGAAGCGGGGGCCGGGATGGTGGTc                                                                                           >  2:364093/1‑140 (MQ=255)                                           gatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTcc                                                                                          <  2:835310/140‑1 (MQ=255)                                           gatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTcc                                                                                          <  1:944705/140‑1 (MQ=255)                                           gatcCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTcc                                                                                          <  1:937244/140‑1 (MQ=255)                                               cgcgcgGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑Ga                                                                                     <  2:62119/140‑1 (MQ=255)                                                 cgcgGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAgg                                                                                   >  1:826985/1‑140 (MQ=255)                                                 cgcgGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAgg                                                                                   >  1:832395/1‑140 (MQ=255)                                                 cgcgGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGGGATGTTGTTCCCG‑GAgg                                                                                   >  2:1419379/1‑140 (MQ=255)                                                  gcggcgGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGc                                                                                  <  2:1169134/140‑1 (MQ=255)                                                  gcggcgGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGc                                                                                  <  2:276478/140‑1 (MQ=255)                                                      cggAGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTAGGGAGGGCGCAAGCCTCTGTTGAAAAGGGAGCCAAGAATTTTTTCCCGCGAGCCGAt                                                                               >  2:966190/1‑140 (MQ=255)                                                             tGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAAc                                                                       <  2:295644/140‑1 (MQ=255)                                                             tGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAAc                                                                       <  2:222808/140‑1 (MQ=255)                                                             tGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAAc                                                                       <  2:426463/140‑1 (MQ=255)                                                              gCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACt                                                                      <  2:572802/140‑1 (MQ=255)                                                                 aaaaCCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGc                                                                   <  1:364093/140‑1 (MQ=255)                                                                 aaaaCCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGCCGAGCGTGCGCAGGCCCATGTTGAAGCGGGGGCCGAGATGTTGGTCCCC‑GGGGCGATTACCGAACTCGc                                                                   >  2:98469/1‑140 (MQ=255)                                                                     ccGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATg                                                               <  1:1436642/140‑1 (MQ=255)                                                                     ccGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCCAACTCGCCATg                                                               >  2:767235/1‑140 (MQ=255)                                                                     ccGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCCATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACCCGCCATg                                                               >  2:257971/1‑140 (MQ=255)                                                                        aTCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTAc                                                            >  2:618149/1‑140 (MQ=255)                                                                             gATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCa                                                       >  1:177931/1‑140 (MQ=255)                                                                                  tgtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GGGGCGATTACCGAACTCGCCATGTACCGcccggttt                                                  >  2:67072/1‑134 (MQ=255)                                                                                  tgtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGGTTACCGAACTCGCCCTGTACCGCCCGGTTg                                                  >  2:920804/1‑140 (MQ=255)                                                                                  tgtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTg                                                  >  1:1288482/1‑140 (MQ=255)                                                                                   gtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATCTACCGCCAGTTTGc                                                 >  1:521139/1‑140 (MQ=255)                                                                                   gtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGc                                                 >  1:1484072/1‑140 (MQ=255)                                                                                   gtgATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGGGCGCAGGCCTATGTTGAAGCGGGGGCCGGGGAGTTGTTCCCG‑GGGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGc                                                 >  1:1181331/1‑140 (MQ=255)                                                                                      aTCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGa                                              <  2:638761/140‑1 (MQ=255)                                                                                        cATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATg                                            <  1:1099690/140‑1 (MQ=255)                                                                                         aTGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGc                                           <  1:1117843/140‑1 (MQ=255)                                                                                         aTGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGc                                           <  1:263325/140‑1 (MQ=255)                                                                                           ggCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCCGTTTGCCGATGCgg                                         >  1:602278/1‑140 (MQ=255)                                                                                              gcgcACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGc                                      <  1:991474/140‑1 (MQ=255)                                                                                                 cACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAgg                                   >  2:1361255/1‑140 (MQ=255)                                                                                                 cACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTGCCGAACTCGCCATGTACCGCCCGTTTGCCGATGCGGGGCAgg                                   >  2:488308/1‑140 (MQ=255)                                                                                                  aCCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAggg                                  >  1:1282790/1‑139 (MQ=255)                                                                                                  aCCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAggg                                  >  1:543243/1‑139 (MQ=255)                                                                                                  aCCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCCCCATGTACCGCCAGTTTGCCGCTGCGGTGCAggg                                  >  2:1413284/1‑139 (MQ=255)                                                                                                    cGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGc                                <  1:115028/140‑1 (MQ=255)                                                                                                      aTGCTCTGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCg                              >  2:1447557/1‑140 (MQ=255)                                                                                                      aTGCTATGGCGGTAGAGGGGCTGGATGCGGCGGGCGCGAGGGCGCCAGCCCCTGTTTAAAAGGGGGCCGAGAAGTTTTTTCCC‑GAGGCGATTTCCCAAATCCCCCCGGACCCCCCGTTTGGCGATGCGGGGCAgggggcg                              >  2:1127857/1‑135 (MQ=255)                                                                                                         ctctGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATc                           <  1:948891/140‑1 (MQ=255)                                                                                                          tctGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATcc                          <  1:1176410/140‑1 (MQ=255)                                                                                                            tGGCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTc                        <  1:30167/140‑1 (MQ=255)                                                                                                              gCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGc                      <  1:767235/140‑1 (MQ=255)                                                                                                              gCGGTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGc                      <  2:832395/140‑1 (MQ=255)                                                                                                                ggTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGGCGAGATGTTGTTCCCG‑GAGGCGATTTCCGGACTCGCCATGTACCGCCAGTTTGCCGATGCGGGGCAGGTGCCGATCCTCGCCa                    >  2:1315565/1‑140 (MQ=255)                                                                                                                 gTAGAGGGGCTGGATGCGGCGGTCGAGCGTGGGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCaa                   <  1:478105/140‑1 (MQ=255)                                                                                                                 gTAGAGGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCCATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCAGGTACCGCCAGTTTTCCGATGCGGTGCAGGTGCCGAACCTCGccca                   >  2:320659/1‑138 (MQ=255)                                                                                                                   agagGGGCTGGATGCGGCGGTCGAGCGTGCGCCGGCCTATGTTGAAGCGGGAGCCGAGATGTTGTTTCCG‑GGGGCGATTACCGGACTCGCCATGTACCGCCCGTTTGTCGGTGCGGGGCAGGTGCCGAACCTCGCCAACa                 >  2:278427/1‑140 (MQ=255)                                                                                                                    gagGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGGGATGTTGTTCCCG‑GAGGGGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGAGGGGGGGCAGGGGCCGAGCCCCGCCAACAt                >  2:435027/1‑140 (MQ=255)                                                                                                                     agGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATc               >  1:1437572/1‑140 (MQ=255)                                                                                                                     agGGGCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATc               >  1:480968/1‑140 (MQ=255)                                                                                                                      ggggCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCa              >  1:348959/1‑140 (MQ=255)                                                                                                                        ggCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTTTGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCAcc            <  1:1152417/140‑1 (MQ=255)                                                                                                                        ggCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCAcc            >  1:1062676/1‑140 (MQ=255)                                                                                                                         gCTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCg           >  1:105548/1‑140 (MQ=255)                                                                                                                          cTGGATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGGTGCGGGGCAGGGGCCGATCCTCGCCAACATCACCgc          >  2:130204/1‑139 (MQ=255)                                                                                                                            ggATGCGGCGGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGGGCCGATCCTCGCCAACATCACCGAAt        >  1:1447946/1‑140 (MQ=255)                                                                                                                                gcggcgGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTgg    <  2:826985/140‑1 (MQ=255)                                                                                                                                  ggcgGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTgggg  >  1:857111/1‑138 (MQ=255)                                                                                                                                  ggcgGTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGGGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTg  >  2:1076110/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         TCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCG‑GAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTG  >  NC_000913/349059‑349327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑