Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,582	C→T	R61Q (CGA→CAA)	cvpA ←	colicin V production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,582	0	C	T	100.0%	276.4 / NA	78	R61Q (CGA→CAA)	cvpA	colicin V production protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/34);  total (44/34)

ACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAC  >  NC_000913/2430445‑2430712                                                                                                                                          |                                                                                                                                   aCGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGa                                                                                                                                  >  2:659448/1‑140 (MQ=255)   gCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAAc                                                                                                                                <  1:1144832/140‑1 (MQ=255)      ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAg                                                                                                                             <  1:748096/140‑1 (MQ=255)            cAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTc                                                                                                                       >  1:1031490/1‑140 (MQ=255)               aCGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTc                                                                                                                    >  1:1036113/1‑140 (MQ=255)                  cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCaaa                                                                                                                 <  2:1400060/140‑1 (MQ=255)                       cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCg                                                                                                            >  2:1178941/1‑140 (MQ=255)                       cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCg                                                                                                            >  1:1151931/1‑140 (MQ=255)                         cccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTa                                                                                                          >  1:8304/1‑140 (MQ=255)                           cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTaaa                                                                                                        <  1:1243500/140‑1 (MQ=255)                           cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTaaa                                                                                                        <  1:1178941/140‑1 (MQ=255)                             aTCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAAcc                                                                                                      >  1:587804/1‑140 (MQ=255)                                 gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGAc                                                                                                  >  2:1375196/1‑140 (MQ=255)                                  tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACt                                                                                                 <  1:340698/140‑1 (MQ=255)                                   gCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTg                                                                                                <  1:299491/140‑1 (MQ=255)                                    ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGa                                                                                               >  2:609638/1‑140 (MQ=255)                                    ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGa                                                                                               <  2:1398111/140‑1 (MQ=255)                                      tGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACa                                                                                             >  2:1339721/1‑140 (MQ=255)                                      tGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACa                                                                                             <  2:796638/140‑1 (MQ=255)                                        aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAgg                                                                                           >  2:1183623/1‑140 (MQ=255)                                        aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGCTCGTCTTCAAAGCCCGTAAACCAGACTGACAgg                                                                                           >  2:702277/1‑140 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:361452/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:764462/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:604545/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:902165/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  2:286097/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:990037/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:365113/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  2:497480/140‑1 (MQ=255)                                         cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                          <  1:1315256/140‑1 (MQ=255)                                           aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTaa                                                                                        <  1:783133/140‑1 (MQ=255)                                            ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                       >  2:419493/1‑140 (MQ=255)                                            ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                       >  2:466482/1‑140 (MQ=255)                                            ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                       >  1:1473337/1‑140 (MQ=255)                                            ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                       >  1:135067/1‑140 (MQ=255)                                             cccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAgt                                                                                      <  2:1252198/140‑1 (MQ=255)                                             cccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAgt                                                                                      <  2:262730/140‑1 (MQ=255)                                                      tcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                             >  2:930399/1‑140 (MQ=255)                                                        caccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGAc                                                                           <  2:1151931/140‑1 (MQ=255)                                                           caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTgg                                                                        <  2:1008253/140‑1 (MQ=255)                                                             aCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGca                                                                      >  2:542848/1‑140 (MQ=255)                                                              cTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaa                                                                     >  2:1176091/1‑140 (MQ=255)                                                                ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaaca                                                                   >  2:440191/1‑140 (MQ=255)                                                                      tCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaaa                                                             >  1:1007710/1‑140 (MQ=255)                                                                         cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                          >  2:308043/1‑140 (MQ=255)                                                                         cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                          >  2:933006/1‑140 (MQ=255)                                                                          gAAGTTTACGATAGCACCAACGATCAGGGTTGTGATAAGCATTTCCGCGATGGCAATCCGATTTTGAACCAGTTCGTCTACAATGCCTGTAAACAAGACTGACAGGAAAGTGTAGTAATGACTGGCAACAAAGAAAGctc                                                         <  2:210805/140‑3 (MQ=255)                                                                          gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcac                                                         <  1:1121660/140‑1 (MQ=255)                                                                           aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcaca                                                        >  1:264720/1‑140 (MQ=255)                                                                                cACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcccc                                                   >  1:315955/1‑140 (MQ=255)                                                                                cACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGAAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcccc                                                   >  1:581881/1‑140 (MQ=255)                                                                                      aGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                             >  1:1196828/1‑140 (MQ=255)                                                                                      aGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                             >  1:546422/1‑140 (MQ=255)                                                                                        cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAcc                                           >  2:1467918/1‑140 (MQ=255)                                                                                        cACCAACGATAAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAcc                                           >  2:828168/1‑140 (MQ=255)                                                                                              cGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAt                                     <  2:1391866/140‑1 (MQ=255)                                                                                                 tCAGGGTAGCGATAAACAGTACCGCGATGGAAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAAc                                  <  2:581881/140‑1 (MQ=255)                                                                                                        aGCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCAc                           >  2:53571/1‑140 (MQ=255)                                                                                                         gCGATAAACAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAATCACAACCCCATGTCACCAGCGATAACGCTTCACg                          >  2:1025068/1‑140 (MQ=255)                                                                                                              aaaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaa                     <  2:716554/140‑1 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  2:1132792/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  1:1448568/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  2:647586/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  1:462089/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  1:465867/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                    >  2:166688/1‑140 (MQ=255)                                                                                                               aaCAGTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCACATGTCACCAGCGATAACGCTTCACGAACaaa                    >  2:867314/1‑140 (MQ=255)                                                                                                                   gTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCCCCAGCGATAACGCTTCACGAACAAAGCcg                <  1:308043/140‑1 (MQ=255)                                                                                                                   gTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcg                >  1:91135/1‑140 (MQ=255)                                                                                                                   gTACCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcg                <  1:930399/140‑1 (MQ=255)                                                                                                                     aCCGCGATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcgcg              <  1:260308/140‑1 (MQ=255)                                                                                                                       cgcgATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGa            >  2:472833/1‑140 (MQ=255)                                                                                                                         cgATGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc          <  1:1339721/140‑1 (MQ=255)                                                                                                                            tGGCAATCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg       <  1:1375196/140‑1 (MQ=255)                                                                                                                                aaTCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTaa   >  2:1327500/1‑140 (MQ=255)                                                                                                                                aaTCCCATTTTGAACCAGTTCGTCTTCAAAACCCGTAAACCAGACTGACAGGTAAGTGGAGTAATGACCGGCAACAAAGAAAGCACAACCCCAAGTCACCAGTGATAACGCTTCACGAACAAAGCCACGGATCCTGCTaa   >  2:577192/1‑140 (MQ=255)                                                                                                                                 aTCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc  >  2:512954/1‑140 (MQ=255)                                                                                                                                 aTCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc  <  1:659448/140‑1 (MQ=255)                                                                                                                                 aTCCCATTTTGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc  <  2:760272/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   ACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAC  >  NC_000913/2430445‑2430712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑