Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,863,847	G→A	A85A (GCG→GCA)	ygbL →	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,863,847	0	G	A	100.0%	285.7 / NA	82	A85A (GCG→GCA)	ygbL	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/45);  total (37/45)

ACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGG  >  NC_000913/2863714‑2863979                                                                                                                                      |                                                                                                                                      aCGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTAt                                                                                                                                 >  1:81125/1‑140 (MQ=255)        tttACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGcaaca                                                                                                                          <  1:514503/140‑1 (MQ=255)          tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAAt                                                                                                                        <  2:929222/140‑1 (MQ=255)          tACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAAt                                                                                                                        <  1:585332/140‑1 (MQ=255)           aCTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATc                                                                                                                       >  2:74877/1‑140 (MQ=255)             tGGCGACTCCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcg                                                                                                                     <  1:326159/140‑1 (MQ=255)             tGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcg                                                                                                                     <  2:741125/140‑1 (MQ=255)             tGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcg                                                                                                                     <  2:1106673/140‑1 (MQ=255)             tGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcg                                                                                                                     <  1:1019406/140‑1 (MQ=255)             tGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcg                                                                                                                     <  1:87061/140‑1 (MQ=255)               gCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcgcg                                                                                                                   <  2:809022/140‑1 (MQ=255)               gCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcgcg                                                                                                                   <  2:38667/140‑1 (MQ=255)               gCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCcgcg                                                                                                                   <  2:352647/140‑1 (MQ=255)                    acCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTa                                                                                                              >  1:848831/1‑140 (MQ=255)                     ccGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTaa                                                                                                             <  2:1058044/140‑1 (MQ=255)                             ttCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                                                                                     <  1:1433725/140‑1 (MQ=255)                             ttCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                                                                                     <  1:566493/140‑1 (MQ=255)                                aTGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGc                                                                                                  >  1:1093998/1‑140 (MQ=255)                                 tGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCa                                                                                                 >  2:57437/1‑140 (MQ=255)                                  gCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCAt                                                                                                <  1:919557/140‑1 (MQ=255)                                  gCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCAt                                                                                                >  1:613479/1‑140 (MQ=255)                                      gggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgc                                                                                            >  2:743338/1‑140 (MQ=255)                                      gggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgc                                                                                            >  2:178953/1‑140 (MQ=255)                                      gggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgc                                                                                            <  2:304886/140‑1 (MQ=255)                                      gggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTACTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgc                                                                                            >  2:466091/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  1:210377/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  2:1030707/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  2:1280028/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  1:1458738/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  2:1367117/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  2:481691/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  1:552485/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  1:506432/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  1:48895/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  1:1094776/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           >  1:396291/1‑140 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  2:81125/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                                                                                           <  1:306458/140‑1 (MQ=255)                                       ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGGGCATTTgca                                                                                           >  2:1499697/1‑140 (MQ=255)                                           aTCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcacagc                                                                                       >  2:1455222/1‑140 (MQ=255)                                                 aTCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                                                                                 >  1:328189/1‑140 (MQ=255)                                                            cTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑Ct                                                                     >  2:639525/1‑140 (MQ=255)                                                              ttCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑Cttt                                                                   <  1:967152/140‑1 (MQ=255)                                                               tCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTc                                                                  <  1:1495573/140‑1 (MQ=255)                                                                      tCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTcctgcctg                                                           >  2:1131565/1‑140 (MQ=255)                                                                       cgccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCctgcctgc                                                          <  1:390748/140‑1 (MQ=255)                                                                       cgccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCctgcctgc                                                          <  1:286888/140‑1 (MQ=255)                                                                       cgccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCctgcctgc                                                          <  1:566165/140‑1 (MQ=255)                                                                       cgccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCctgcctgc                                                          <  2:1404738/140‑1 (MQ=255)                                                                        gccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCa                                                         >  2:256474/1‑140 (MQ=255)                                                                         ccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCaa                                                        >  1:407344/1‑140 (MQ=255)                                                                         ccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCaa                                                        >  2:1473080/1‑140 (MQ=255)                                                                         ccgcGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCaa                                                        >  1:1304311/1‑140 (MQ=255)                                                                            cgGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAggg                                                     <  2:552485/140‑1 (MQ=255)                                                                            cgGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAggg                                                     <  2:210377/140‑1 (MQ=255)                                                                               aTGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTg                                                  >  2:118457/1‑140 (MQ=255)                                                                                 ggCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGa                                                <  2:135146/140‑1 (MQ=255)                                                                                 ggCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGa                                                <  2:223201/140‑1 (MQ=255)                                                                                   cGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGAca                                              >  2:1445233/1‑140 (MQ=255)                                                                                       tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGAcagcag                                          <  1:743338/140‑1 (MQ=255)                                                                                       tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGAcagcag                                          >  2:1443993/1‑140 (MQ=255)                                                                                          ttAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCaa                                       <  2:548245/140‑1 (MQ=255)                                                                                                 gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTAtt                                >  1:107712/1‑140 (MQ=255)                                                                                                  tgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGTG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTc                               >  2:460409/1‑140 (MQ=255)                                                                                                   gACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCg                              >  1:1395771/1‑140 (MQ=255)                                                                                                    aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGt                             >  2:721601/1‑140 (MQ=255)                                                                                                    aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGt                             <  1:1455222/140‑1 (MQ=255)                                                                                                    aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGACGGCGCTGCCTTGCCTG‑CTGCAGGTGCACAGCAGCAACAACGTTATTccg                             >  1:1122815/1‑138 (MQ=255)                                                                                                    aCAAACCCTCGAAAGAGGTGCTC‑‑TCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTcc                           >  1:59445/1‑140 (MQ=255)                                                                                                        aCCCTCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGt                         <  2:328189/140‑1 (MQ=255)                                                                                                            tCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGAAGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTcac                     >  2:1166568/1‑140 (MQ=255)                                                                                                            tCGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGAGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTcac                     >  2:1035270/1‑140 (MQ=255)                                                                                                             cGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTcaca                    <  1:694385/140‑1 (MQ=255)                                                                                                             cGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTcaca                    <  1:481691/140‑1 (MQ=255)                                                                                                             cGAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTcaca                    <  2:848831/140‑1 (MQ=255)                                                                                                              gAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                   <  1:616046/140‑1 (MQ=255)                                                                                                              gAAAGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCacac                   <  1:478129/140‑1 (MQ=255)                                                                                                               aaaGAGGTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACAcc                  <  1:1473080/140‑1 (MQ=255)                                                                                                                    ggTGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACg             <  2:1492519/140‑1 (MQ=255)                                                                                                                      tGCTCTTTCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtg           >  2:762643/1‑140 (MQ=255)                                                                                                                            ttCATCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATg     <  2:396291/140‑1 (MQ=255)                                                                                                                               aTCTGGCACTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCgg  <  2:59445/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCG‑CTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGG  >  NC_000913/2863714‑2863979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑