Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,746	T→C	C55R (TGT→CGT)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,746	0	T	C	98.6%	272.1 / NA	73	C55R (TGT→CGT)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (23/49);  minor base G (0/1);  total (23/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTATCCGCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACA  >  NC_000913/2935610‑2935878                                                                                                                                         |                                                                                                                                     tGGTATCCGCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAgcgtg                                                                                                                                   <  2:1083228/140‑1 (MQ=255) tGGTATCCGCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAgcgtg                                                                                                                                   <  2:338845/140‑1 (MQ=255)    tATCCGCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCa                                                                                                                                <  1:610207/140‑1 (MQ=255)         gCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTcc                                                                                                                           <  1:567430/140‑1 (MQ=255)         gCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTcc                                                                                                                           <  2:119364/140‑1 (MQ=255)         gCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTcc                                                                                                                           <  2:593203/140‑1 (MQ=255)         gCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTcc                                                                                                                           <  2:1383621/140‑1 (MQ=255)              gTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGGGTGTCATTTCCGATAc                                                                                                                      <  2:1251059/140‑1 (MQ=255)              gTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATAc                                                                                                                      >  2:567340/1‑140 (MQ=255)               ttATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATAcc                                                                                                                     <  1:1020693/140‑1 (MQ=255)               ttATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATAcc                                                                                                                     <  2:673577/140‑1 (MQ=255)                tATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCt                                                                                                                    <  1:312842/140‑1 (MQ=255)                tATTGACGGTCGTCGCATGGGCGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCt                                                                                                                    >  1:68533/1‑140 (MQ=255)                     aCGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATc                                                                                                               <  1:14493/140‑1 (MQ=255)                      cGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcg                                                                                                              <  1:1004807/140‑1 (MQ=255)                       ggTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcgc                                                                                                             <  1:1045039/140‑1 (MQ=255)                        gtcgtcGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcgcg                                                                                                            <  1:593338/140‑1 (MQ=255)                        gtcgtcGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcgcg                                                                                                            <  1:449059/140‑1 (MQ=255)                         tcgtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCgg                                                                                                           >  2:1185268/1‑140 (MQ=255)                         tcgtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCgg                                                                                                           >  2:731414/1‑140 (MQ=255)                         tcgtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCgg                                                                                                           >  2:1053514/1‑140 (MQ=255)                         tcgtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCgg                                                                                                           >  1:493697/1‑140 (MQ=255)                          cgtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCggg                                                                                                          >  2:1066631/1‑140 (MQ=255)                           gtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGt                                                                                                         <  2:1167418/140‑1 (MQ=255)                           gtcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGCGTCATTTCCGATACCTGTATCGCgggc                                                                                                         >  2:279181/1‑139 (MQ=255)                            tcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTa                                                                                                        <  2:489616/140‑1 (MQ=255)                            tcgCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTa                                                                                                        >  2:816813/1‑140 (MQ=255)                                   ggTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcg                                                                                                              >  2:911765/1‑127 (MQ=255)                                   ggTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATcg                                                                                                              <  1:911765/127‑1 (MQ=255)                                   ggTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGa                                                                                                 >  2:1095943/1‑140 (MQ=255)                                         cGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCgc                                                                                           >  1:674318/1‑140 (MQ=255)                                              gTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCtt                                                                                      >  2:655291/1‑140 (MQ=255)                                               tCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTg                                                                                     >  2:267644/1‑140 (MQ=255)                                               tCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTg                                                                                     <  1:1391287/140‑1 (MQ=255)                                                cGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGc                                                                                    <  2:1153839/140‑1 (MQ=255)                                                        gaaCAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGaaaa                                                                            <  1:293121/140‑1 (MQ=255)                                                              acaaTGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTcag                                                                      >  1:177833/1‑140 (MQ=255)                                                              acaaTGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTcag                                                                      <  1:131665/140‑1 (MQ=255)                                                               caaTGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTcagc                                                                     <  1:523567/140‑1 (MQ=255)                                                                aaTGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGTCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTcagca                                                                    >  1:433422/1‑140 (MQ=255)                                                                  tGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGt                                                                  <  1:816813/140‑1 (MQ=255)                                                                    aaTATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCa                                                                <  2:693230/140‑1 (MQ=255)                                                                    aaTATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCa                                                                <  1:731414/140‑1 (MQ=255)                                                                    aaTATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCa                                                                <  1:123444/140‑1 (MQ=255)                                                                    aaTATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCa                                                                <  2:68533/140‑1 (MQ=255)                                                                    aaTATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCa                                                                <  2:1194060/140‑1 (MQ=255)                                                                      tatGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGa                                                              >  1:561099/1‑140 (MQ=255)                                                                        tGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAAt                                                            >  1:105075/1‑140 (MQ=255)                                                                         ggCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATg                                                           >  2:897380/1‑140 (MQ=255)                                                                         ggCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATg                                                           >  2:1104241/1‑140 (MQ=255)                                                                         ggCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATg                                                           >  2:45409/1‑140 (MQ=255)                                                                           cGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTa                                                         <  2:208890/140‑1 (MQ=255)                                                                           cGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTa                                                         <  1:1185268/140‑1 (MQ=255)                                                                                gCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCt                                                    <  1:1361780/140‑1 (MQ=255)                                                                                   aCGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCAc                                                 <  2:774776/140‑1 (MQ=255)                                                                                    cGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCAcc                                                >  2:593658/1‑140 (MQ=255)                                                                                      gCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCAt                                              <  2:316642/140‑1 (MQ=255)                                                                                            ctgctgACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGt                                        <  1:1211332/140‑1 (MQ=255)                                                                                                  aCCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGcc                                  <  2:22689/140‑1 (MQ=255)                                                                                                   ccGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACATGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCTCCATTACGGTAACGCCt                                 <  2:679945/140‑1 (MQ=255)                                                                                                      ggaAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGc                              <  2:674318/139‑1 (MQ=255)                                                                                                      agaACCTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGc                              <  2:820524/140‑1 (MQ=255)                                                                                                      agaAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGc                              <  1:524859/140‑1 (MQ=255)                                                                                                       gaATCTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTGTGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCt                             <  2:390077/140‑1 (MQ=255)                                                                                                         aaCTGCGCCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTgg                           >  1:671988/1‑140 (MQ=255)                                                                                                               gcCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTAt                     <  1:254248/140‑1 (MQ=255)                                                                                                               gcCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTAt                     <  2:728290/140‑1 (MQ=255)                                                                                                               gcCATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTAt                     <  2:711532/140‑1 (MQ=255)                                                                                                                ccATGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATg                    <  1:843629/140‑1 (MQ=255)                                                                                                                   tGCCTGCGGAGCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATG‑AGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAg                <  2:433422/140‑1 (MQ=255)                                                                                                                             gCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCAt       <  1:848789/140‑1 (MQ=255)                                                                                                                             gCTGCCGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCAt       <  1:655291/140‑1 (MQ=255)                                                                                                                                 ccGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGAc   <  1:1095943/140‑1 (MQ=255)                                                                                                                                  cGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACa  <  1:1053514/140‑1 (MQ=255)                                                                                                                                  cGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACa  <  1:194827/140‑1 (MQ=255)                                                                                                                                  cGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACa  <  1:1066631/140‑1 (MQ=255)                                                                                                                                  cGTCGAGCGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACa  <  2:493697/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGGTATCCGCCCGGTTATTGACGGTCGTCGCATGGGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACA  >  NC_000913/2935610‑2935878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑