Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	382,567	T→C	C298C (TGT→TGC)	insD‑1 →	IS2 element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	382,567	0	T	C	100.0%	223.0 / NA	60	C298C (TGT→TGC)	insD‑1	IS2 element protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/25);  total (35/25)

GCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAA  >  NC_000913/382454‑382697                                                                                                                  |                                                                                                                                   gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAAtt                                                                                                          <  1:1425574/140‑1 (MQ=11)   agagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTAc                                                                                                        <  1:173165/140‑1 (MQ=17)                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                       >  1:135615/1‑140 (MQ=255)                     taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc                                                                                      >  2:1465489/1‑140 (MQ=255)                       aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa                                                                                    <  1:565637/140‑1 (MQ=255)                       aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCa                                                                                    <  1:348471/140‑1 (MQ=255)                         gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTTCCTATCAGGCAGTTTGCGCCAAt                                                                                  <  2:1355573/140‑1 (MQ=255)                         gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                  >  1:590802/1‑140 (MQ=255)                         gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                  <  1:1410132/140‑1 (MQ=255)                         gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                  <  2:775407/140‑1 (MQ=255)                           aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                >  2:693587/1‑140 (MQ=255)                           aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCa                                                                                <  1:769587/140‑1 (MQ=255)                            tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCAc                                                                               >  1:93439/1‑140 (MQ=255)                            tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCAc                                                                               >  1:904447/1‑140 (MQ=255)                            tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCAc                                                                               >  1:1124423/1‑140 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACt                                                                              >  2:796490/1‑140 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACt                                                                              >  1:1329499/1‑140 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACt                                                                              >  1:611672/1‑140 (MQ=255)                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACt                                                                              >  1:1199213/1‑140 (MQ=255)                               cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAc                                                                            >  2:1064086/1‑140 (MQ=255)                                aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAcc                                                                           <  2:631978/140‑1 (MQ=255)                                   cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTTCCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                        <  2:619569/140‑1 (MQ=255)                                   cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGa                                                                        <  1:1064086/140‑1 (MQ=255)                                       tAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCt                                                                    >  2:617993/1‑140 (MQ=255)                                        aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATTGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTg                                                                   <  2:1082845/140‑1 (MQ=255)                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATa                                                               >  2:649794/1‑140 (MQ=255)                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATa                                                               >  2:372182/1‑140 (MQ=255)                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATa                                                               >  2:602243/1‑140 (MQ=255)                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACg                                                            >  2:1227323/1‑140 (MQ=255)                                                  gTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCa                                                         <  2:1178748/140‑1 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAcc                                                       >  1:883364/1‑140 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAcc                                                       >  2:1271728/1‑140 (MQ=255)                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAcc                                                       >  1:394962/1‑140 (MQ=255)                                                         ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGAc                                                  <  2:1490313/140‑1 (MQ=255)                                                            gcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGACACCATGACaaa                                               >  1:868784/1‑140 (MQ=255)                                                             ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAAt                                              >  1:702869/1‑140 (MQ=255)                                                             ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAAt                                              >  2:713464/1‑140 (MQ=255)                                                                 gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACgg                                          >  2:157960/1‑140 (MQ=255)                                                                 gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACgg                                          >  2:510463/1‑140 (MQ=255)                                                                      tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTAtt                                     <  1:1059602/140‑1 (MQ=255)                                                                           gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCgcgc                                >  1:769547/1‑140 (MQ=255)                                                                              gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                             >  2:381047/1‑140 (MQ=255)                                                                                aGCGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATa                           <  2:1333722/140‑1 (MQ=255)                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaac                         >  2:80638/1‑140 (MQ=255)                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaac                         >  1:1229375/1‑140 (MQ=255)                                                                                   gggCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGACACCATGACAAATACGGGTATTCGCGCCGATaaca                        <  2:868784/140‑1 (MQ=255)                                                                                    ggCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaa                       >  2:1166794/1‑140 (MQ=255)                                                                                     gCTTGTAATGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaac                      <  2:135615/140‑1 (MQ=255)                                                                                           aaTGGGTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCAcc                <  1:144413/140‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa            <  2:590802/140‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa            >  2:22589/1‑140 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa            <  2:769547/140‑1 (MQ=255)                                                                                               ggTTAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa            >  1:1055418/1‑140 (MQ=255)                                                                                                 ttAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGt          <  2:361779/140‑1 (MQ=255)                                                                                                 ttAAGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGt          <  1:1494566/140‑1 (MQ=255)                                                                                                    aGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtc       >  2:356691/1‑140 (MQ=255)                                                                                                    aGTGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtc       >  1:944553/1‑140 (MQ=255)                                                                                                      tGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat     <  1:372182/140‑1 (MQ=255)                                                                                                      tGATAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat     <  1:1465489/140‑1 (MQ=255)                                                                                                         tAACAGATGCCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCACCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaa  >  2:1101562/1‑140 (MQ=255)                                                                                                                  |                                                                                                                                   GCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAA  >  NC_000913/382454‑382697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑