Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,962,539	T→C	T76A (ACA→GCA)	ppdC ←	putative YgdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,962,539	0	T	C	100.0%	211.5 / NA	59	T76A (ACA→GCA)	ppdC	putative YgdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/32);  total (27/32)

ATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGAT  >  NC_000913/2962405‑2962675                                                                                                                                       |                                                                                                                                         aTTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCa                                                                                                                                     <  1:440045/140‑1 (MQ=255)   tGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGCTGCCTGCAtt                                                                                                                                   >  2:624901/1‑140 (MQ=255)   tGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGGAACGCTGATGCTGACACATCCCCCCCGCGATGCCTGCAtt                                                                                                                                   >  2:520226/1‑140 (MQ=255)   tGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTTCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCAtt                                                                                                                                   >  1:429109/1‑140 (MQ=255)     gAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCg                                                                                                                                 <  1:1254203/140‑1 (MQ=255)       aTGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGt                                                                                                                               <  2:822581/140‑1 (MQ=255)                ccTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATTTCACCTTCTCTGCCCCCGGGGGAAACTAACGTAAAGCAGGAGCTTACACAACCCGCCTGCGCTGCCTGCATTCGGGTGGCCAGcc                                                                                                                      >  2:645435/1‑140 (MQ=255)                    aaCATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGtt                                                                                                                  >  2:104380/1‑140 (MQ=255)                    aaCATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGtt                                                                                                                  >  1:822388/1‑140 (MQ=255)                    aaCATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCCGCGGTGCCCGCCTTCGGGTGAACTGCCAGtt                                                                                                                  >  2:816404/1‑140 (MQ=255)                      cATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTgg                                                                                                                <  1:1000620/140‑1 (MQ=255)                      cATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTgg                                                                                                                <  1:152559/140‑1 (MQ=255)                         aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCCGCGATGCCTGCATTCGGTTGACCTGCCCGTTGGCAg                                                                                                             >  2:1367572/1‑140 (MQ=255)                          gCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAgg                                                                                                            >  1:226553/1‑140 (MQ=255)                          gCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCCGGGGAAACTAGCGTAAAGCTGATGCTGCCACATCCCGCCCGCGATGGCTGCCATCGGGTGACCCGCCCGTTGGCCgg                                                                                                            >  2:906402/1‑140 (MQ=255)                          gCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCCCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAgg                                                                                                            >  1:1221711/1‑140 (MQ=255)                                ccTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGa                                                                                                      >  2:1372198/1‑140 (MQ=255)                                ccTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGGAACGCTGATGCTGACACATCCCGCCCGCGATGCCTGCATTTGGTTGACCTGCCAGGTGGCAGGTGGCGa                                                                                                      >  2:488559/1‑140 (MQ=255)                                    actactGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATc                                                                                                  >  2:385544/1‑140 (MQ=255)                                           aCGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCa                                                                                           >  2:907085/1‑140 (MQ=255)                                           aCGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCa                                                                                           >  1:548282/1‑140 (MQ=255)                                           aCGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCa                                                                                           >  1:365703/1‑140 (MQ=255)                                             gATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGt                                                                                         <  1:366424/140‑1 (MQ=255)                                              aTTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGtt                                                                                        >  2:1437416/1‑140 (MQ=255)                                                tCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTTCCCCCCGGGGAAACTAGCGTAACGCCGATGGTGACACATCCCGCCTGCCATGCCTGCATTCGGTTGACCTGCCAGTTGGCCGGGGGCGAAAACgcgcgccggtgg                                                                                      >  2:1489364/1‑134 (MQ=255)                                                tCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAACGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGc                                                                                      >  2:737161/1‑140 (MQ=255)                                                      aaTGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTtgc                                                                                >  1:330402/1‑140 (MQ=255)                                                       aTGCAGGCGGGTCATCTCTCCCTCTCCGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACACTCCCCCTGCGACGCCTGCATTCCGTTGACCTGCCCGTTGGCAGGTGGCGAAATCGCGCGCCGTTGCGTTtgct                                                                               >  2:371489/1‑140 (MQ=255)                                                        tGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTtgctg                                                                              >  1:223887/1‑140 (MQ=255)                                                        tGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTtgctg                                                                              >  1:1265948/1‑140 (MQ=255)                                                          cAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTgcc                                                                            >  1:1095590/1‑140 (MQ=255)                                                           aGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTgcca                                                                           <  1:1196212/140‑1 (MQ=255)                                                             gCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTgccagc                                                                         <  1:867143/140‑1 (MQ=255)                                                                ggTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCAt                                                                      <  2:176167/140‑1 (MQ=255)                                                                  tCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGGAACGCCGATGCTGACACATCCCCCCCGCGATGCCTGCCTTCGGGTGACCCGCCCGTTGGCCGGGGGGGAAAACGCGCGCAGGTGCGTTTGCTGCCCGCCATgc                                                                    >  2:1461891/1‑140 (MQ=255)                                                                    aTCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATgccg                                                                  >  1:51503/1‑140 (MQ=255)                                                                    aTCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCCGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCCGGTGCGTTTGCCGGCAGCCATgccc                                                                  >  2:750600/1‑139 (MQ=255)                                                                     tctcGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATgccgc                                                                 <  1:1188042/140‑1 (MQ=255)                                                                       tcGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCa                                                               <  2:1169098/140‑1 (MQ=255)                                                                         gCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCaga                                                             <  1:816404/140‑1 (MQ=255)                                                                         gCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCaga                                                             <  1:488559/140‑1 (MQ=255)                                                                          ccTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCagag                                                            <  1:104380/140‑1 (MQ=255)                                                                              ctctGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGCCACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGt                                                        <  2:1221711/140‑1 (MQ=255)                                                                              ctctGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGt                                                        >  1:738666/1‑140 (MQ=255)                                                                                ctGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTg                                                      <  1:907085/140‑1 (MQ=255)                                                                                 tGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTgg                                                     >  2:708901/1‑140 (MQ=255)                                                                                   cccccGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTa                                                   >  1:979319/1‑140 (MQ=255)                                                                                        gggTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGt                                              >  2:1185784/1‑140 (MQ=255)                                                                                         ggTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGtt                                             >  1:345521/1‑140 (MQ=255)                                                                                            gAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTct                                          <  2:884577/140‑1 (MQ=255)                                                                                            gAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTct                                          <  2:1095590/140‑1 (MQ=255)                                                                                            gAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTct                                          <  2:566155/140‑1 (MQ=255)                                                                                            gAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTct                                          <  2:365703/140‑1 (MQ=255)                                                                                                 tggCGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTgg                                     <  2:1156280/138‑1 (MQ=255)                                                                                                    cGTAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCaa                                  >  2:115427/1‑140 (MQ=255)                                                                                                      tAACGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGa                                <  1:906402/140‑1 (MQ=255)                                                                                                         cGCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTg                             <  2:822388/140‑1 (MQ=255)                                                                                                          gCTGATGCTGACACATCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGt                            >  1:970424/1‑140 (MQ=255)                                                                                                                     cacaTCCCGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGtt                 <  1:645435/140‑1 (MQ=255)                                                                                                                          cccGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTg            >  2:359082/1‑140 (MQ=255)                                                                                                                            cGCCTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGa          >  1:462258/1‑140 (MQ=255)                                                                                                                              ccTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGaaa        <  1:1437416/140‑1 (MQ=255)                                                                                                                              ccTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGaaa        >  2:86970/1‑140 (MQ=255)                                                                                                                              ccTGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGaaa        >  2:193877/1‑140 (MQ=255)                                                                                                                                tGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAAcc      <  2:614500/140‑1 (MQ=255)                                                                                                                                tGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAAcc      >  2:574461/1‑140 (MQ=255)                                                                                                                                tGCGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAAcc      >  2:130737/1‑140 (MQ=255)                                                                                                                                  cGATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCg    <  1:385544/140‑1 (MQ=255)                                                                                                                                   gATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGa   <  1:737161/140‑1 (MQ=255)                                                                                                                                   gATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGa   <  2:330402/140‑1 (MQ=255)                                                                                                                                   gATGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGa   <  1:1489364/140‑1 (MQ=255)                                                                                                                                    aTGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGAt  <  1:520226/140‑1 (MQ=255)                                                                                                                                    aTGCCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGAt  <  2:226553/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGAT  >  NC_000913/2962405‑2962675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑