Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,844	A→G	Y262C (TAC→TGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,844	0	A	G	100.0%	329.3 / NA	91	Y262C (TAC→TGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/48);  total (43/48)

ACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAA  >  NC_000913/3016708‑3016978                                                                                                                                         |                                                                                                                                       accaGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTgcgc                                                                                                                                     >  2:732648/1‑140 (MQ=255)   caGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTgcgcgc                                                                                                                                   <  2:355383/140‑1 (MQ=255)   caGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTgcgcgc                                                                                                                                   <  1:205965/140‑1 (MQ=255)    aGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGcgcgcg                                                                                                                                  <  1:1476772/140‑1 (MQ=255)    aGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGcgcgcg                                                                                                                                  <  2:471752/140‑1 (MQ=255)    aGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGcgcgcg                                                                                                                                  <  2:987243/140‑1 (MQ=255)    aGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGcgcgcg                                                                                                                                  <  1:679858/140‑1 (MQ=255)    aGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGcgcgcg                                                                                                                                  <  1:617119/140‑1 (MQ=255)     gTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCgt                                                                                                                                 >  1:735524/1‑140 (MQ=255)      tATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTCTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCgtg                                                                                                                                >  2:796628/1‑140 (MQ=255)         ggTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTc                                                                                                                             >  1:1001511/1‑140 (MQ=255)           tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGt                                                                                                                           >  1:577019/1‑140 (MQ=255)           tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGt                                                                                                                           >  1:496499/1‑140 (MQ=255)           tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGt                                                                                                                           <  1:1379470/140‑1 (MQ=255)           tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGt                                                                                                                           >  1:282689/1‑140 (MQ=255)           tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGt                                                                                                                           >  2:675153/1‑140 (MQ=255)            gCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTg                                                                                                                          <  1:1312080/140‑1 (MQ=255)             cATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTga                                                                                                                         >  1:58484/1‑140 (MQ=255)             cATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTga                                                                                                                         >  1:590713/1‑140 (MQ=255)              aTGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTgag                                                                                                                        <  1:1339143/140‑1 (MQ=255)                ggCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGAt                                                                                                                      >  1:1434135/1‑140 (MQ=255)                ggCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGAt                                                                                                                      >  1:942825/1‑140 (MQ=255)                  cGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATcc                                                                                                                    >  2:557079/1‑140 (MQ=255)                  cGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATcc                                                                                                                    >  1:1208954/1‑140 (MQ=255)                    tCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACTTGCTGGAAGAAAAAGGGCTCAACTCCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTc                                                                                                                  <  2:1085293/140‑1 (MQ=255)                    tCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTc                                                                                                                  <  2:1045466/140‑1 (MQ=255)                     cACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCg                                                                                                                 >  2:381760/1‑140 (MQ=255)                          tctcCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTc                                                                                                            <  1:745440/140‑1 (MQ=255)                              caccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCg                                                                                                        <  2:548877/140‑1 (MQ=255)                              caccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTAGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCg                                                                                                        >  1:931361/1‑140 (MQ=255)                                ccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGt                                                                                                      <  2:1040893/140‑1 (MQ=255)                                  aTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGttt                                                                                                    >  1:785168/1‑140 (MQ=255)                                  aTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGttt                                                                                                    <  2:591245/140‑1 (MQ=255)                                  aTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGttt                                                                                                    >  1:1405036/1‑140 (MQ=255)                                   tGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTc                                                                                                   >  2:353256/1‑140 (MQ=255)                                   tGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTc                                                                                                   >  1:1226728/1‑140 (MQ=255)                                   tGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTc                                                                                                   >  1:66655/1‑140 (MQ=255)                                    gCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCg                                                                                                  <  2:360765/140‑1 (MQ=255)                                     cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCgg                                                                                                 <  2:1019789/140‑1 (MQ=255)                                     cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCgg                                                                                                 >  1:174563/1‑140 (MQ=255)                                      aCGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGt                                                                                                >  1:729896/1‑140 (MQ=255)                                      aCGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGt                                                                                                >  1:111248/1‑140 (MQ=255)                                      aCGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGt                                                                                                >  2:1232731/1‑140 (MQ=255)                                        ggCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTa                                                                                              >  2:768004/1‑140 (MQ=255)                                        ggCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTa                                                                                              >  2:942224/1‑140 (MQ=255)                                         gCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTAc                                                                                             <  2:1046590/140‑1 (MQ=255)                                          cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                            >  2:1047925/1‑140 (MQ=255)                                          cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                            >  1:124752/1‑140 (MQ=255)                                           tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                           >  2:426552/1‑140 (MQ=255)                                           tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                           <  1:416800/140‑1 (MQ=255)                                           tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                           <  1:381760/140‑1 (MQ=255)                                            gTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATa                                                                                          <  1:1215493/140‑1 (MQ=255)                                             tcctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAg                                                                                         >  1:1112783/1‑140 (MQ=255)                                              cctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAgg                                                                                        <  1:153891/140‑1 (MQ=255)                                                  cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTa                                                                                    >  1:1404672/1‑140 (MQ=255)                                                   gCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTaa                                                                                   <  1:1405729/140‑1 (MQ=255)                                                    cATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTaaa                                                                                  >  2:697296/1‑140 (MQ=255)                                                      tGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaag                                                                                <  1:393107/140‑1 (MQ=255)                                                           tCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAagag                                                                           <  2:1495882/140‑1 (MQ=255)                                                            cGAAGCCATTTGCCGCTACATGCTAGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGt                                                                          <  1:759549/140‑1 (MQ=255)                                                                     ttGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATc                                                                 <  2:414448/140‑1 (MQ=255)                                                                      tGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCa                                                                <  2:491775/140‑1 (MQ=255)                                                                      tGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCa                                                                <  2:735524/140‑1 (MQ=255)                                                                       gCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCAc                                                               <  2:1355033/140‑1 (MQ=255)                                                                       gCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCAc                                                               <  1:1227256/140‑1 (MQ=255)                                                                          gCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGAc                                                            <  2:111248/140‑1 (MQ=255)                                                                           cTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGAcc                                                           <  2:1434135/140‑1 (MQ=255)                                                                             aCATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTc                                                         >  2:1495126/1‑140 (MQ=255)                                                                                tGCTGGAAGAAAAAGGGCTCAACCCCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg                                                      <  1:51951/140‑1 (MQ=255)                                                                                tGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg                                                      <  1:353256/140‑1 (MQ=255)                                                                                  cTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt                                                    <  1:1047925/140‑1 (MQ=255)                                                                                      aagaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACg                                                >  2:857597/1‑140 (MQ=255)                                                                                      aagaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACg                                                <  2:1001511/140‑1 (MQ=255)                                                                                        gaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa                                              <  2:496499/140‑1 (MQ=255)                                                                                             aGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCAc                                         >  1:572841/1‑140 (MQ=255)                                                                                                gCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTgg                                      <  2:1208954/140‑1 (MQ=255)                                                                                                 cTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGa                                     >  1:1248961/1‑140 (MQ=255)                                                                                                     acacCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATg                                 >  1:1400198/1‑140 (MQ=255)                                                                                                      cacCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGc                                <  2:1405036/140‑1 (MQ=255)                                                                                                      cacCTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGc                                <  1:675153/140‑1 (MQ=255)                                                                                                         cTTTGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTgg                             >  1:843732/1‑140 (MQ=255)                                                                                                           ttGTGAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                           <  1:1160181/140‑1 (MQ=255)                                                                                                            tgtgAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAAc                          >  1:1375374/1‑140 (MQ=255)                                                                                                             gtgAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACg                         <  2:282689/140‑1 (MQ=255)                                                                                                             gtgAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACg                         <  1:851699/140‑1 (MQ=255)                                                                                                              tgAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGc                        <  1:942224/140‑1 (MQ=255)                                                                                                              tgAAACTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGc                        >  2:898732/1‑140 (MQ=255)                                                                                                                aaaCTTAACCCGACCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCt                      >  2:732099/1‑140 (MQ=255)                                                                                                                            aCCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGc          >  2:633433/1‑140 (MQ=255)                                                                                                                            aCCTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGc          <  2:124752/140‑1 (MQ=255)                                                                                                                             ccTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCa         <  1:1222398/140‑1 (MQ=255)                                                                                                                             ccTTACTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCa         >  2:1465060/1‑140 (MQ=255)                                                                                                                                 aCTGGGGTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAg     <  1:732648/140‑1 (MQ=255)                                                                                                                                    ggggTGCGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGaaa  <  2:1226728/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAA  >  NC_000913/3016708‑3016978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑