Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,729	T→C	F236L (TTC→CTC)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,729	0	T	C	100.0%	189.5 / NA	51	F236L (TTC→CTC)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/18);  total (33/18)

GCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGC  >  NC_000913/3208592‑3208863                                                                                                                                          |                                                                                                                                       gCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTc                                                                                                                                      >  1:484854/1‑140 (MQ=255)   tCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCt                                                                                                                                    <  2:680937/140‑1 (MQ=255)      tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCCTTTTCTTGACGGCGATGGCACCCAAATTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTTCTCgg                                                                                                                                 >  2:1276640/1‑140 (MQ=255)      tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCCAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGGGACTGGCTCCTCgg                                                                                                                                 >  1:195704/1‑140 (MQ=255)      tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCgg                                                                                                                                 >  1:1301500/1‑140 (MQ=255)        catcatGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGa                                                                                                                               <  1:1078829/140‑1 (MQ=255)           catGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGt                                                                                                                            >  2:1132322/1‑140 (MQ=255)             tgtgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTg                                                                                                                          >  1:85813/1‑140 (MQ=255)             tgtgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTg                                                                                                                          <  2:1097153/140‑1 (MQ=255)               tgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATAGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGcc                                                                                                                        >  2:458402/1‑140 (MQ=255)                ggATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCg                                                                                                                       <  2:75365/140‑1 (MQ=255)                 gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  1:1050946/1‑140 (MQ=255)                 gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  2:736486/1‑140 (MQ=255)                 gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  2:736487/1‑140 (MQ=255)                  aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     <  2:715463/140‑1 (MQ=255)                  aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     <  2:557697/140‑1 (MQ=255)                  aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     >  1:557864/1‑140 (MQ=255)                     gggATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAg                                                                                                                  >  1:1478424/1‑140 (MQ=255)                     gggATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAg                                                                                                                  >  1:47141/1‑140 (MQ=255)                       gATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCa                                                                                                                >  2:1269196/1‑140 (MQ=255)                        aTTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCAt                                                                                                               >  1:1410442/1‑140 (MQ=255)                            ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTa                                                                                                           >  1:1445069/1‑140 (MQ=255)                            ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTa                                                                                                           >  1:365766/1‑140 (MQ=255)                                cGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTgg                                                                                                       >  1:818202/1‑140 (MQ=255)                                      cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgc                                                                                                 >  1:656061/1‑140 (MQ=255)                                      cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgc                                                                                                 >  1:1001455/1‑140 (MQ=255)                                       gTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct                                                                                                <  1:1485984/140‑1 (MQ=255)                                        tGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgctg                                                                                               <  1:551429/140‑1 (MQ=255)                                                gcCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAACAGCGCATCTCAGGCCACGCTGAGTTGGGGCGACTGGCTACTCGGGATGATGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctg                                                                                       >  1:799881/1‑140 (MQ=255)                                                 ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctgg                                                                                      <  2:1335515/140‑1 (MQ=255)                                                 ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctgg                                                                                      <  2:557864/140‑1 (MQ=255)                                                     tttCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctggctgg                                                                                  <  2:1050946/140‑1 (MQ=255)                                                        cTTGACGGCGATGGCACTAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCTCGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCtt                                                                               <  2:1450873/140‑1 (MQ=255)                                                        cTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCtt                                                                               <  2:1301500/140‑1 (MQ=255)                                                         ttGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTa                                                                              <  2:795866/140‑1 (MQ=255)                                                           gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACg                                                                            >  1:25919/1‑140 (MQ=255)                                                            aCGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGt                                                                           >  2:1256603/1‑140 (MQ=255)                                                             cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  2:47141/140‑1 (MQ=255)                                                             cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  1:1269196/140‑1 (MQ=255)                                                             cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  2:1070532/140‑1 (MQ=255)                                                             cGGCGATAGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  1:458402/140‑1 (MQ=255)                                                                cGAGGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTGGTGTCGACTGGCTCCTCGGGCTGTTGCCGCTGAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTg                                                                       >  2:781466/1‑140 (MQ=255)                                                                   tGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTAc                                                                    >  1:356030/1‑140 (MQ=255)                                                                                      aaTTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTg                                                 >  1:175282/1‑140 (MQ=255)                                                                                        ttGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCCGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGc                                               >  2:702892/1‑140 (MQ=255)                                                                                                          cATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                             >  1:435832/1‑140 (MQ=255)                                                                                                          cATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                             >  2:1205679/1‑140 (MQ=255)                                                                                                                       gcTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGaat                >  1:890322/1‑139 (MQ=255)                                                                                                                       gcTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                >  1:1166154/1‑140 (MQ=255)                                                                                                                                     ccTGGCTCCTCGGGATGCTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGCACTGAAGCCTGGCGAACAGGGGCCGCGCCGGGCAGCGACGGAACTGCAGGCAATGGGc  >  2:570949/2‑140 (MQ=255)                                                                                                                                     aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:273144/1‑140 (MQ=255)                                                                                                                                     aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:546557/1‑140 (MQ=255)                                                                                                                                     aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:454336/1‑140 (MQ=255)                                                                                                                                     aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAACGGGc  >  1:114784/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGC  >  NC_000913/3208592‑3208863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑