BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,234,091	T→C	A18A (GCA→GCG)	higA ←	antitoxin/DNA‑binding transcriptional repressor HigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,234,091	0	T	C	100.0%	436.1 / NA	116	A18A (GCA→GCG)	higA	antitoxin/DNA‑binding transcriptional repressor HigA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (61/55); total (61/55)

Only 100 of 118 total aligned reads displayed.

CCCACGCGGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGTGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTT  >  NC_000913/3233964‑3234222

cccACGCGGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGtt                                                                                                                         <  1:301909/140‑1 (MQ=255)

cccACGCGGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGtt                                                                                                                         >  2:1202606/1‑140 (MQ=255)

  cACGCGGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAg                                                                                                                       <  1:1383862/140‑1 (MQ=255)

  cACGCGGTTA‑TTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGc                                                                                                                      >  2:873391/1‑140 (MQ=255)

    cgcgGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCt                                                                                                                     <  2:399146/140‑1 (MQ=255)

    cgcgGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCt                                                                                                                     >  2:1433292/1‑140 (MQ=255)

        gTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTtc                                                                                                                 >  2:1079246/1‑140 (MQ=255)

          tATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTtctc                                                                                                               <  2:253254/140‑1 (MQ=255)

            ttttGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCt                                                                                                             <  2:291732/140‑1 (MQ=255)

             tttGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCTTGGGTGTTTTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTg                                                                                                            <  1:302120/140‑1 (MQ=255)

             tttGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTg                                                                                                            <  1:1046127/140‑1 (MQ=255)

                  cacacaCCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGc                                                                                                       <  1:1411733/140‑1 (MQ=255)

                     acacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAg                                                                                                    <  2:1158072/140‑1 (MQ=255)

                     acacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAg                                                                                                    >  1:421843/1‑140 (MQ=255)

                      cacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGa                                                                                                   >  2:1182720/1‑140 (MQ=255)

                      cacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGa                                                                                                   >  2:1357892/1‑140 (MQ=255)

                      cacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGa                                                                                                   <  1:373579/140‑1 (MQ=255)

                      cacCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGa                                                                                                   >  1:159137/1‑140 (MQ=255)

                       cccAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGAt                                                                                                  <  2:42406/139‑1 (MQ=255)

                          aGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTc                                                                                               <  2:304810/140‑1 (MQ=255)

                           gATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCTCCAGAAAAGGCGCCACAGCAGTTAGCTCTTCTCCTGCTTGCAAGATGTCg                                                                                              >  2:1007750/1‑140 (MQ=255)

                            aTCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCgg                                                                                             <  1:427116/140‑1 (MQ=255)

                                aGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaat                                                                                         >  2:814026/1‑140 (MQ=255)

                                 gCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatc                                                                                        >  1:78391/1‑140 (MQ=255)

                                 gCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatc                                                                                        >  1:729374/1‑140 (MQ=255)

                                 gCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatc                                                                                        >  1:128072/1‑140 (MQ=255)

                                 gCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGCGCATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatc                                                                                        >  2:652620/1‑140 (MQ=255)

                                   aaGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatcgc                                                                                      >  1:574865/1‑140 (MQ=255)

                                   aaGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatcgc                                                                                      >  1:896217/1‑140 (MQ=255)

                                       ggTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGgcaatcgcaatc                                                                                  <  1:1311899/140‑1 (MQ=255)

                                         ttttCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCAt                                                                                <  2:793588/140‑1 (MQ=255)

                                          tttCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCAtt                                                                               >  1:1217520/1‑140 (MQ=255)

                                           ttCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCAttt                                                                              >  1:268879/1‑140 (MQ=255)

                                              aGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCAtttttt                                                                           <  2:667188/140‑1 (MQ=255)

                                              aGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCAtttttt                                                                           <  2:1311555/140‑1 (MQ=255)

                                               ggATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTc                                                                          <  1:1079246/140‑1 (MQ=255)

                                                gATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTcc                                                                         <  1:1202606/140‑1 (MQ=255)

                                                gATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTcc                                                                         <  2:864530/140‑1 (MQ=255)

                                                   cGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCt                                                                      <  1:661771/140‑1 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCTTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    <  2:548264/140‑1 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    >  2:1027311/1‑140 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    >  1:1141332/1‑140 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    >  2:680176/1‑140 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    >  2:40704/1‑140 (MQ=255)

                                                     ttGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCACCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTa                                                                    <  1:1225434/140‑1 (MQ=255)

                                                        agcagcAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTa                                                                 >  1:886603/1‑140 (MQ=255)

                                                             cagATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATg                                                            <  1:636851/140‑1 (MQ=255)

                                                               gatgatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          >  1:125927/1‑140 (MQ=255)

                                                               gatgatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          >  2:1008763/1‑140 (MQ=255)

                                                               gatgatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          >  1:1094480/1‑140 (MQ=255)

                                                               gatgatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          >  1:595225/1‑140 (MQ=255)

                                                               gatgatCTACCAGTTCCAGCCCCTGGGTGTATTGTTCCTCGTTCTGAATCCCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          <  2:242/140‑1 (MQ=255)

                                                               gaggatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGaa                                                          <  1:1357892/137‑1 (MQ=255)

                                                                  gatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAg                                                       <  1:719952/140‑1 (MQ=255)

                                                                  gatCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAg                                                       >  1:530433/1‑140 (MQ=255)

                                                                   atCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGc                                                      <  2:1103108/140‑1 (MQ=255)

                                                                    tCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCg                                                     >  1:728125/1‑140 (MQ=255)

                                                                       aCCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTa                                                  >  1:671373/1‑140 (MQ=255)

                                                                        ccAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTaa                                                 >  1:1061530/1‑140 (MQ=255)

                                                                          aGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAg                                               <  1:123414/140‑1 (MQ=255)

                                                                          aGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAg                                               >  2:1379369/1‑140 (MQ=255)

                                                                          aGTTCCAGCGCCTGGGAGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGCACGATGAACAACGGTACAg                                               >  2:1347818/1‑140 (MQ=255)

                                                                             tCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGaaa                                            >  1:35232/1‑140 (MQ=255)

                                                                              ccAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAAt                                           >  1:1397090/1‑140 (MQ=255)

                                                                              ccAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAAt                                           >  2:574831/1‑140 (MQ=255)

                                                                                aGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCg                                         >  1:777082/1‑140 (MQ=255)

                                                                                  cgcCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTa                                       <  1:1038384/140‑1 (MQ=255)

                                                                                    ccTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTAtt                                     >  1:908557/1‑140 (MQ=255)

                                                                                     cTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTc                                    >  2:646737/1‑140 (MQ=255)

                                                                                       gggCGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCtt                                  >  1:339054/1‑140 (MQ=255)

                                                                                          tgtATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTAt                               <  2:128072/140‑1 (MQ=255)

                                                                                           gtATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATg                              <  2:421843/140‑1 (MQ=255)

                                                                                             aTTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCATTCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCg                            >  2:1182814/1‑140 (MQ=255)

                                                                                             aTTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCg                            >  2:1387817/1‑140 (MQ=255)

                                                                                             aTTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCg                            >  2:862693/1‑140 (MQ=255)

                                                                                              ttGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGt                           >  1:1470330/1‑140 (MQ=255)

                                                                                               tGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTc                          >  1:453292/1‑140 (MQ=255)

                                                                                               tGTTCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTc                          <  1:1461213/140‑1 (MQ=255)

                                                                                                 ttCCTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCAt                        <  2:225611/140‑1 (MQ=255)

                                                                                                   ccTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATaa                      >  2:797208/1‑140 (MQ=255)

                                                                                                   ccTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATaa                      >  1:775724/1‑140 (MQ=255)

                                                                                                    cTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAAc                     <  2:78391/140‑1 (MQ=255)

                                                                                                    cTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAAc                     <  1:824101/140‑1 (MQ=255)

                                                                                                    cTCGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAAc                     <  1:1347818/140‑1 (MQ=255)

                                                                                                      cGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACtt                   <  2:896217/140‑1 (MQ=255)

                                                                                                      cGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACtt                   <  2:1277125/140‑1 (MQ=255)

                                                                                                      cGTTCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACtt                   <  1:537778/140‑1 (MQ=255)

                                                                                                         tCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCAc                <  2:352893/140‑1 (MQ=255)

                                                                                                         tCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCAc                >  2:286291/1‑140 (MQ=255)

                                                                                                         tCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCAc                <  2:1094480/140‑1 (MQ=255)

                                                                                                         tCTGAATACCCGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCAc                <  1:821113/140‑1 (MQ=255)

                                                                                                                 cccGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAg        <  2:595225/140‑1 (MQ=255)

                                                                                                                 cccGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAg        >  2:19514/1‑140 (MQ=255)

                                                                                                                 cccGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAg        <  2:860931/140‑1 (MQ=255)

                                                                                                                  ccGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGc       >  2:64044/1‑140 (MQ=255)

                                                                                                                   cGCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCAc                <  1:1263286/130‑1 (MQ=255)

                                                                                                                    gCCAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCAc     <  2:686372/140‑1 (MQ=255)

                                                                                                                     ccAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACt    >  2:1152709/1‑140 (MQ=255)

                                                                                                                     ccAGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAAGCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACt    >  2:1185627/1‑140 (MQ=255)

                                                                                                                       aGAAAAGGCGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACttt  >  1:1317016/1‑140 (MQ=255)

CCCACGCGGTTATTTTGGCACACACCAGATCCAGCAAGGGGTTTTCAGGATCGTTGAGCAGCAGATGATCTACCAGTTCCAGCGCCTGGGTGTATTGTTCCTCGTTCTGAATACCCGCCAGAAAAGGTGCCACAGCAGTTAGCTTTTCTCCTGCTTGCAAGATGTCGGCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTT  >  NC_000913/3233964‑3234222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`