Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,483	T→C	E457E (GAA→GAG)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,483	0	T	C	100.0%	345.7 / NA	91	E457E (GAA→GAG)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/44);  total (47/44)

ACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCG  >  NC_000913/3468348‑3468619                                                                                                                                        |                                                                                                                                         aCCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGt                                                                                                                                      >  1:310846/1‑140 (MQ=255)   cGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTAt                                                                                                                                    >  1:980988/1‑140 (MQ=255)   cGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTAt                                                                                                                                    >  2:592545/1‑140 (MQ=255)   cGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTAt                                                                                                                                    >  2:275964/1‑140 (MQ=255)     gTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTAttt                                                                                                                                  >  2:1304343/1‑140 (MQ=255)      tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCCCCTTTCACGTACATAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttc                                                                                                                                 <  1:242965/140‑1 (MQ=255)      tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttc                                                                                                                                 >  2:1211086/1‑140 (MQ=255)      tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttc                                                                                                                                 >  2:14353/1‑140 (MQ=255)       ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttct                                                                                                                                >  2:1334314/1‑140 (MQ=255)       ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttct                                                                                                                                >  2:974345/1‑140 (MQ=255)         cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATttcttc                                                                                                                              >  2:1292676/1‑140 (MQ=255)           ttttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCa                                                                                                                            <  2:1156655/140‑1 (MQ=255)           ttttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCa                                                                                                                            <  1:918376/140‑1 (MQ=255)           ttttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCa                                                                                                                            <  2:31524/140‑1 (MQ=255)                     ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATcg                                                                                                                  <  2:734561/140‑1 (MQ=255)                     ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATcg                                                                                                                  >  1:1064878/1‑140 (MQ=255)                       accaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATcgcc                                                                                                                <  2:1115962/140‑1 (MQ=255)                          agagCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCgccgcc                                                                                                             <  2:632025/140‑1 (MQ=255)                            agCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCgccgcctg                                                                                                           <  1:113487/140‑3 (MQ=255)                            agCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCg                                                                                                           <  1:1068071/140‑1 (MQ=255)                                gggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGAc                                                                                                       <  2:747318/140‑1 (MQ=255)                                gggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGAc                                                                                                       >  2:1062095/1‑140 (MQ=255)                                 ggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACa                                                                                                      >  1:944581/1‑140 (MQ=255)                                  gTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACaa                                                                                                     >  1:1298282/1‑140 (MQ=255)                                   ttGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACaaa                                                                                                    <  1:368594/140‑1 (MQ=255)                                    tGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAg                                                                                                   <  2:980988/140‑1 (MQ=255)                                     gTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGTTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGc                                                                                                  <  1:109354/140‑1 (MQ=255)                                     gTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGc                                                                                                  <  2:605934/140‑1 (MQ=255)                                     gTCACTTTGCGTCCAGAACAACGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGc                                                                                                  >  1:437311/1‑140 (MQ=255)                                      tCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGCCAAAGcc                                                                                                 >  1:1450199/1‑140 (MQ=255)                                         cTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCa                                                                                              <  2:310846/140‑1 (MQ=255)                                          tttGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCAt                                                                                             <  1:854964/140‑1 (MQ=255)                                           ttGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGGCGCCCGGGACAAAGCCCCATg                                                                                            >  1:325769/1‑140 (MQ=255)                                           ttGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATg                                                                                            >  2:165901/1‑140 (MQ=255)                                           ttGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATg                                                                                            >  1:792943/1‑140 (MQ=255)                                             gCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCg                                                                                          <  2:1193004/140‑1 (MQ=255)                                               gTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTc                                                                                        >  1:468244/1‑140 (MQ=255)                                                          cgcTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAg                                                                             <  2:1489637/140‑1 (MQ=255)                                                          cgcTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAg                                                                             <  2:958335/140‑1 (MQ=255)                                                          cgcTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAg                                                                             <  2:321738/140‑1 (MQ=255)                                                            cTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGaa                                                                           >  1:1349920/1‑140 (MQ=255)                                                               cATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGtt                                                                        >  2:113982/1‑140 (MQ=255)                                                               cATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGtt                                                                        >  2:204963/1‑140 (MQ=255)                                                                 tAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGtttt                                                                      <  1:14353/140‑1 (MQ=255)                                                                   aGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTtat                                                                    <  1:592545/140‑1 (MQ=255)                                                                    gCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTtata                                                                   >  2:645199/1‑140 (MQ=255)                                                                    gCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTtata                                                                   <  2:739182/140‑1 (MQ=255)                                                                         cccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAAtgtg                                                              >  2:214150/1‑140 (MQ=255)                                                                         cccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAAtgtg                                                              >  1:1129671/1‑140 (MQ=255)                                                                         cccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAAtgtg                                                              >  1:646662/1‑140 (MQ=255)                                                                         cccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAAtgtg                                                              <  2:116590/140‑1 (MQ=255)                                                                         cccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAAtgtg                                                              >  1:493460/1‑140 (MQ=255)                                                                          ccATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGc                                                             <  1:1334314/140‑1 (MQ=255)                                                                           cATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCa                                                            <  1:1211086/140‑1 (MQ=255)                                                                           cATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCa                                                            <  1:1062095/140‑1 (MQ=255)                                                                           cATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCa                                                            <  1:275964/140‑1 (MQ=255)                                                                           cATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCa                                                            <  1:392080/140‑1 (MQ=255)                                                                           cATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCa                                                            <  2:1090300/140‑1 (MQ=255)                                                                              caacaacaaCGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCg                                                         >  1:144411/1‑140 (MQ=255)                                                                                   acaacGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTtata                                                    <  2:792943/140‑1 (MQ=255)                                                                                   acaacGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTtata                                                    <  1:1081900/140‑1 (MQ=255)                                                                                   acaacGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTtata                                                    <  2:22832/140‑1 (MQ=255)                                                                                    caacGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATaa                                                   >  1:608385/1‑140 (MQ=255)                                                                                       cgcgATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTtata                                                    >  1:399345/1‑136 (MQ=255)                                                                                       cgcgATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTtata                                                    <  2:399345/136‑1 (MQ=255)                                                                                       cgcgATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCt                                                >  1:144249/1‑140 (MQ=255)                                                                                       cgcgATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCt                                                >  2:540364/1‑140 (MQ=255)                                                                                                  ttCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTc                                     <  2:944581/140‑1 (MQ=255)                                                                                                   tCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTcc                                    <  1:919073/140‑1 (MQ=255)                                                                                                    cACGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCcg                                   <  1:868078/140‑1 (MQ=255)                                                                                                      cGTACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCcgcg                                 >  2:495963/1‑140 (MQ=255)                                                                                                        tACACAGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGATAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCt                               <  1:1292676/140‑1 (MQ=255)                                                                                                             aGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACg                          <  2:1064878/140‑1 (MQ=255)                                                                                                             aGTGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACg                          <  1:1304343/140‑1 (MQ=255)                                                                                                               tGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCt                        >  1:1335347/1‑140 (MQ=255)                                                                                                               tGGATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCAACGATGCTGTCCGCGCTGTACGCt                        >  1:530911/1‑140 (MQ=255)                                                                                                                 gATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCttt                      >  2:285699/1‑140 (MQ=255)                                                                                                                 gATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCttt                      >  2:772496/1‑140 (MQ=255)                                                                                                                 gATGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCttt                      >  1:1050341/1‑140 (MQ=255)                                                                                                                  aTGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTg                     >  1:1453496/1‑140 (MQ=255)                                                                                                                   tGCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGc                    >  1:229878/1‑140 (MQ=255)                                                                                                                    gCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCt                   <  1:251566/140‑1 (MQ=255)                                                                                                                    gCGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCt                   >  2:229869/1‑140 (MQ=255)                                                                                                                     cGGACCATGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTg                  >  1:791971/1‑140 (MQ=255)                                                                                                                            tGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGa           >  2:719254/1‑140 (MQ=255)                                                                                                                            tGCTTTCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTAAGCTTTGCTGGCCTGGa           >  2:610501/1‑140 (MQ=255)                                                                                                                                ttCGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAAcc       <  2:144249/140‑1 (MQ=255)                                                                                                                                  cGCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGGTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCAt     >  1:1123639/1‑140 (MQ=255)                                                                                                                                   gCCGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATc    <  2:1484125/140‑1 (MQ=255)                                                                                                                                    ccGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATcc   >  2:1175355/1‑140 (MQ=255)                                                                                                                                     cGGCTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCg  <  1:974345/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGTATTTCTTCCACGGGTTATCGCCGCCCGGGACAAAGCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCG  >  NC_000913/3468348‑3468619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑