breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,209,8270TG38.5% 51.0 / 11.2 39noncoding (54/76 nt)gltVtRNA‑Glu
*NC_0009133,556,2050AC36.6% 72.2 / 15.8 41S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,906,7690AC34.6% 87.3 / 13.9 52Y98D (TAT→GAT) rlmA23S rRNA m(1)G745 methyltransferase
*NC_0009133,745,3970AC33.9% 111.6 / 13.7 66L198F (TTA→TTCyiaN2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
*NC_0009133,556,2070TC33.3% 61.0 / 15.4 42S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009133,943,4880TG33.3% 49.0 / 12.6 36noncoding (54/76 nt)gltUtRNA‑Glu
*NC_000913372,6400TG32.8% 124.8 / 20.1 61L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009134,604,3440CT31.9% 106.1 / 34.1 72S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,740,9090TG31.1% 154.9 / 19.4 74V402G (GTG→GGG) avtAvaline‑‑pyruvate aminotransferase
*NC_0009134,568,5040TG30.9% 200.4 / 15.5 95T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_000913650,8090TG30.2% 110.6 / 14.5 53T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009132,001,9180AC28.6% 142.0 / 21.3 63intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009134,032,7310TG28.4% 141.3 / 20.3 67G80G (GGT→GGGyigZIMPACT family member YigZ
*NC_0009132,667,1260AC28.3% 199.7 / 27.8 92Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_000913388,3360AC28.2% 153.9 / 10.3 72N181T (AAC→ACC) tauDtaurine dioxygenase
*NC_0009131,550,6630AC27.9% 97.2 / 13.5 43N68T (AAC→ACC) fdnHformate dehydrogenase N subunit beta
*NC_0009133,619,2680TG27.3% 148.1 / 11.5 66V26G (GTA→GGA) rhsBrhs element protein RhsB
*NC_0009132,782,5090AC26.3% 263.3 / 24.1 114V73G (GTA→GGA) ypjAadhesin‑like autotransporter YpjA
*NC_0009133,844,2190TG26.1% 220.5 / 19.2 92F86V (TTT→GTT) adeDadenine deaminase
*NC_0009133,206,4350CT25.0% 71.8 / 13.9 36intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 120780566 (0.740)17 (0.220) 16/246 3.2 24.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 45 (0.570)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 4542682 =31 (0.350)15 (0.180) 14/260 3.9 27.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 50 (0.600)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269034 (0.380)13 (0.160) 13/260 4.1 24.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 49 (0.590)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 1397238NA (NA)14 (0.160) 10/278 5.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
* ? NC_000913 374921 =NA (NA)8 (0.130) 5/192 5.5 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1979271 =0 (0.000)10 (0.110) 8/278 6.0 100% intergenic (‑56/‑482) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 4373261 =NA (NA)13 (0.180) 5/228 6.3 NA intergenic (+99/+104) yjeI/yjeJ DUF4156 domain‑containing lipoprotein YjeI/protein YjeJ
?NC_000913 4374533 = NA (NA)intergenic (‑299/+96) yjeJ/epmB protein YjeJ/lysine 2,3‑aminomutase
* ? NC_000913 = 374963NA (NA)4 (0.070) 3/192 6.6 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 142850098 (1.110)4 (0.050)
+CGC
3/270 8.2 4.0% noncoding (295/1196 nt) IS5 repeat region
?NC_000913 2067066 = NA (NA)noncoding (288/1195 nt) IS5 repeat region