Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	837,442	(A)8→7	intergenic (+6/+223)	hcxB → / ← ybiJ	hydroxycarboxylate dehydrogenase B/DUF1471 domain‑containing protein YbiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	837,435	0	A	.	100.0%	317.7 / NA	73	coding (1085/1086 nt)	hcxB	hydroxycarboxylate dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/43);  total (30/43)

AACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATC  >  NC_000913/837302‑837564                                                                                                                                      |                                                                                                                                  aaCACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTaaaaaaa                                                                                                                             >  1:845330/1‑140 (MQ=255)  acacGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTaaaaaaag                                                                                                                            >  1:588454/1‑139 (MQ=255)   cacGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTaaaaaaagc                                                                                                                           <  2:979109/140‑3 (MQ=255)   cacGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTaaaaaaagc                                                                                                                           <  2:292801/140‑3 (MQ=255)      gcgTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCg                                                                                                                       >  1:586333/1‑140 (MQ=255)       cgTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGt                                                                                                                      <  2:1228591/140‑1 (MQ=255)        gTCGCGAACGGCAGGAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTc                                                                                                                     <  2:595405/140‑1 (MQ=255)        gTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTc                                                                                                                     <  2:1080787/140‑1 (MQ=255)         tCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTcc                                                                                                                    >  1:407906/1‑140 (MQ=255)                 ggCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCgg                                                                                                            <  1:1463687/140‑1 (MQ=255)                  gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGGTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGa                                                                                                           <  1:775667/140‑1 (MQ=255)                  gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGa                                                                                                           <  2:588454/140‑1 (MQ=255)                  gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGa                                                                                                           <  1:217136/140‑1 (MQ=255)                  gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGa                                                                                                           <  1:782911/140‑1 (MQ=255)                  gCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGa                                                                                                           <  2:1456905/140‑1 (MQ=255)                         cAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCaa                                                                                                    <  1:1227507/140‑1 (MQ=255)                         cAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCaa                                                                                                    <  1:1094401/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  2:1147456/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  1:965908/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  2:1290155/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  >  1:510594/1‑140 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  2:261108/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  2:453234/140‑1 (MQ=255)                           ggggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAc                                                                                                  <  1:210789/140‑1 (MQ=255)                            gggATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCGGTGGCGGACGGGCAAACa                                                                                                 >  1:700822/1‑140 (MQ=255)                                ttCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAggg                                                                                             <  2:578479/140‑1 (MQ=255)                                ttCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAggg                                                                                             <  2:586333/140‑1 (MQ=255)                                 tCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAgggg                                                                                            >  1:470050/1‑139 (MQ=255)                                 tCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGt                                                                                            <  1:672837/140‑1 (MQ=255)                                 tCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGt                                                                                            >  1:1213956/1‑140 (MQ=255)                                     cTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACa                                                                                        <  1:551270/140‑1 (MQ=255)                                       ggATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATa                                                                                      <  2:1309171/140‑1 (MQ=255)                                       ggATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATa                                                                                      >  2:1264416/1‑140 (MQ=255)                                        gATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAg                                                                                     >  1:1180747/1‑140 (MQ=255)                                        gATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAAAAAGGGTAACATAg                                                                                     >  1:1494909/1‑140 (MQ=255)                                         aTGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAgg                                                                                    <  2:1223934/140‑1 (MQ=255)                                          tGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGGAACATAGGa                                                                                   >  2:1259611/1‑140 (MQ=255)                                           gCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGAt                                                                                  <  1:1495688/140‑1 (MQ=255)                                           gCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGAt                                                                                  >  2:683083/1‑140 (MQ=255)                                           gCGGGAAGCTGGCAGGCCATTTGTGAAGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGAt                                                                                  <  2:1328344/140‑1 (MQ=255)                                             gggAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCa                                                                                >  1:821643/1‑140 (MQ=255)                                             gggAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCa                                                                                >  1:660326/1‑140 (MQ=255)                                                aaGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATg                                                                             <  2:1131233/140‑1 (MQ=255)                                                aaGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATg                                                                             <  1:89238/140‑1 (MQ=255)                                                 aGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGa                                                                            <  2:848199/140‑1 (MQ=255)                                                    tGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAAAATAGGAGCAATGAggg                                                                         >  2:55475/1‑140 (MQ=255)                                                     ggCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGt                                                                        <  2:1270684/140‑1 (MQ=255)                                                      gCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGtt                                                                       <  1:503896/140‑1 (MQ=255)                                                                       cAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGt                                                      >  1:1335038/1‑140 (MQ=255)                                                                        aGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTc                                                     >  1:726374/1‑140 (MQ=255)                                                                         gcgcGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCg                                                    >  1:137396/1‑140 (MQ=255)                                                                          cgcgGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg                                                   >  1:9249/1‑140 (MQ=255)                                                                          cgcgGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg                                                   <  1:451603/140‑1 (MQ=255)                                                                          cgcgGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg                                                   >  2:231449/1‑140 (MQ=255)                                                                          cgcgGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCgg                                                   >  1:598557/1‑140 (MQ=255)                                                                          cgcgGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAAGGTAACATATGAACAATGAAGGTTATAGCAAATGCATCTGTCgg                                                   >  2:7989/1‑140 (MQ=255)                                                                             ggCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCaa                                                <  2:1447819/140‑1 (MQ=255)                                                                                  aTTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacaga                                           <  2:407906/140‑1 (MQ=255)                                                                                  aTTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacaga                                           <  2:845330/140‑1 (MQ=255)                                                                                  aTTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacaga                                           <  2:9249/140‑1 (MQ=255)                                                                                  aTTGGTATGCCGGAAGAGACGTTGCAGACTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacaga                                           >  2:361888/1‑140 (MQ=255)                                                                                    tGGTATGCCGGAAGAGACGTTGCTGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacagaca                                         <  1:99246/140‑1 (MQ=255)                                                                                    tGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAacagaca                                         <  2:358644/140‑1 (MQ=255)                                                                                    tGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGAACAATGAGGGGTAGAGCATATGCGTCTGTCGGCAAacagaca                                         >  1:839245/1‑140 (MQ=255)                                                                                        aTGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGa                                     <  1:621907/140‑1 (MQ=255)                                                                                         tGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGaa                                    >  1:946257/1‑140 (MQ=255)                                                                                            cGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATa                                 >  1:1512210/1‑140 (MQ=255)                                                                                                  agaCGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTtgtg                           <  2:1180747/140‑1 (MQ=255)                                                                                                    aCGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCt                         >  1:806866/1‑140 (MQ=255)                                                                                                       ttGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGa                      <  2:1213956/140‑1 (MQ=255)                                                                                                        tGCAGGCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGTCCGTCCAGTGGCGGACGGGCAAACAAGGGGAACATAGGATCAATGAGGGGTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGAc                     >  2:892344/1‑140 (MQ=255)                                                                                                            ggCTTTCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGGAACATAAGAGCAATGAGGGCTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAg                 >  2:711389/1‑140 (MQ=255)                                                                                                               tttCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGt              >  2:564449/1‑140 (MQ=255)                                                                                                                ttCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTa             <  2:510594/140‑1 (MQ=255)                                                                                                                ttCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTa             <  1:28757/140‑1 (MQ=255)                                                                                                                ttCTGTCAGCAGTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTa             <  2:946257/140‑1 (MQ=255)                                                                                                                           gTTAGCCAGCT‑AAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGGAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGGCGTAGCGTAAAAGCCTGaac  >  2:986953/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                  AACACGCGTCGCGAACGGCAGAAGCAGGGGATTCCACTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCAGCGCGGCAGATTGGTATGCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAAAAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGCAAACAGACAGGGAAATACTTGTGCTGGACGTAGCGTAAACGCCTGATC  >  NC_000913/837302‑837564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑