Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,297,389	(G)5→6	coding (750/1041 nt)	yraQ ←	permease family protein YraQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,297,384	1	.	G	100.0%	249.6 / NA	67	H252P (CAT→CCT)	yraQ	permease family protein YraQ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (30/37);  total (30/37)

CCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑GGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGT  >  NC_000913/3297247‑3297516                                                                                                                                           |                                                                                                                                     ccGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑gg                                                                                                                                    <  2:127350/140‑1 (MQ=255) ccGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑gg                                                                                                                                    <  1:1233552/140‑1 (MQ=255)  cGGAGCGGTTCCCATCCCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggg                                                                                                                                   <  2:691824/140‑1 (MQ=255)  cGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggg                                                                                                                                   <  2:1453355/140‑1 (MQ=255)    gAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggggg                                                                                                                                 >  2:13514/1‑140 (MQ=255)       cGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggggggaa                                                                                                                              <  2:1410711/140‑4 (MQ=255)        ggTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggggggaat                                                                                                                             <  2:187025/140‑5 (MQ=255)         gTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑ggggggaata                                                                                                                            >  2:1397778/1‑135 (MQ=255)           tCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAAc                                                                                                                           <  1:463894/140‑1 (MQ=255)                  ccTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGAcgc                                                                                                                    <  2:219842/140‑1 (MQ=255)                  ccTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGAcgc                                                                                                                    <  2:110670/140‑1 (MQ=255)                      ccAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGc                                                                                                                >  1:794080/1‑140 (MQ=255)                      ccAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGc                                                                                                                >  1:345494/1‑140 (MQ=255)                      ccAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGc                                                                                                                >  2:1472718/1‑140 (MQ=255)                      ccAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGc                                                                                                                >  2:664393/1‑140 (MQ=255)                       cAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCg                                                                                                               <  1:551565/140‑1 (MQ=255)                        aGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCgg                                                                                                              <  1:157945/140‑1 (MQ=255)                        aGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCgg                                                                                                              <  1:367007/140‑1 (MQ=255)                        aGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCgg                                                                                                              <  1:297033/140‑1 (MQ=255)                        aGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCgg                                                                                                              <  1:1248984/140‑1 (MQ=255)                        aGCATCATCGCTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCgg                                                                                                              <  2:1358092/140‑1 (MQ=255)                             catcGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCAccc                                                                                                         >  1:191288/1‑140 (MQ=255)                             catcGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCAccc                                                                                                         <  1:159096/140‑1 (MQ=255)                             catcGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCAccc                                                                                                         <  1:848353/140‑1 (MQ=255)                             catcGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCAccc                                                                                                         <  1:1056041/140‑1 (MQ=255)                                cGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAac                                                                                                      >  2:617571/1‑140 (MQ=255)                                cGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAac                                                                                                      >  2:963923/1‑140 (MQ=255)                                  tttGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAacac                                                                                                    <  2:209229/140‑1 (MQ=255)                                  tttGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAacac                                                                                                    <  2:1228663/140‑1 (MQ=255)                                  tttGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAacac                                                                                                    <  1:1405187/140‑1 (MQ=255)                                  tttGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAacac                                                                                                    <  1:1132660/140‑1 (MQ=255)                                          aTCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTg                                                                                            >  1:1464120/1‑140 (MQ=255)                                           tCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGc                                                                                           >  2:438149/1‑140 (MQ=255)                                           tCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGc                                                                                           >  1:1438963/1‑140 (MQ=255)                                            cGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCa                                                                                          <  1:935296/140‑1 (MQ=255)                                                 tttCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGAt                                                                                     <  2:226067/140‑1 (MQ=255)                                                  ttCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACGCCAGTACTGCAAGGATg                                                                                    <  2:477634/140‑1 (MQ=255)                                                  ttCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATg                                                                                    <  2:262482/140‑1 (MQ=255)                                                   tCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGt                                                                                   >  2:1120412/1‑140 (MQ=255)                                                       ctgcCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAAc                                                                               <  1:182531/140‑1 (MQ=255)                                                         gcCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCg                                                                             <  1:428521/140‑1 (MQ=255)                                                         gcCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCg                                                                             <  2:974517/140‑1 (MQ=255)                                                          ccGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCgg                                                                            <  1:1397778/140‑1 (MQ=255)                                                             tGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGAt                                                                         >  2:838954/1‑140 (MQ=255)                                                              ggaaaggACAACCAAGCACCCTGCTCCCGCCACCGCCCCCCCCCCCATCAGGCGGTTATCGCCAGCCCCATCGGCTT‑GGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCg                                                                       <  2:871320/134‑1 (MQ=255)                                                               ggAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCg                                                                       <  2:794080/140‑1 (MQ=255)                                                                 aaTGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTa                                                                     <  1:863414/140‑1 (MQ=255)                                                                                          gCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGccc                                            <  1:1219096/140‑1 (MQ=255)                                                                                               cGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGccc                                       >  1:260888/1‑140 (MQ=255)                                                                                                 ccaccaCCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCa                                     <  1:395511/140‑1 (MQ=255)                                                                                                    ccacccacATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCg                                  <  2:318256/140‑1 (MQ=255)                                                                                                         cacaTCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAAAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaa                             <  1:664393/140‑1 (MQ=255)                                                                                                            aTCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaaa                          >  2:1350512/1‑140 (MQ=255)                                                                                                            aTCAGGCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaaa                          >  1:1505748/1‑140 (MQ=255)                                                                                                                ggCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAccc                      >  1:921733/1‑140 (MQ=255)                                                                                                                 gCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAcccc                     >  2:1291110/1‑139 (MQ=255)                                                                                                                 gCTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCg                     <  1:1120412/140‑1 (MQ=255)                                                                                                                  cTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAccgcc                    >  2:1001401/1‑137 (MQ=255)                                                                                                                  cTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGc                    <  2:1438963/140‑1 (MQ=255)                                                                                                                  cTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGc                    >  1:1477382/1‑140 (MQ=255)                                                                                                                  cTGTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGc                    >  1:8923/1‑140 (MQ=255)                                                                                                                    gTTATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGccc                  <  1:438149/140‑1 (MQ=255)                                                                                                                     ttATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACGCCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAGCCCGCCCt                 <  2:191288/140‑1 (MQ=255)                                                                                                                     ttATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCt                 <  2:1464120/140‑1 (MQ=255)                                                                                                                      tATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCtg                <  1:617571/140‑1 (MQ=255)                                                                                                                      tATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCtg                >  1:1252282/1‑140 (MQ=255)                                                                                                                      tATCGACAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCtg                >  2:655282/1‑140 (MQ=255)                                                                                                                            cAGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTc          <  2:257206/140‑1 (MQ=255)                                                                                                                             aGCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTcc         >  2:1408783/1‑140 (MQ=255)                                                                                                                              gCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCg        <  2:345494/140‑1 (MQ=255)                                                                                                                              gCACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCg        <  1:838954/140‑1 (MQ=255)                                                                                                                               cACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCgg       >  2:48409/1‑140 (MQ=255)                                                                                                                               cACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCgg       >  1:354352/1‑140 (MQ=255)                                                                                                                               cACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCgg       >  1:296103/1‑140 (MQ=255)                                                                                                                               cACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCgg       >  1:1503461/1‑140 (MQ=255)                                                                                                                               cACCATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCgg       >  1:1336749/1‑140 (MQ=255)                                                                                                                                  cATCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTAt    >  1:504021/1‑140 (MQ=255)                                                                                                                                    tCGGCATGGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGt  >  1:153211/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                     CCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCAT‑GGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGT  >  NC_000913/3297247‑3297516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑