Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,5040TG34.8% 225.7 / 23.5 119T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (19/58);  new base G (41/0);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 2.32e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 125 total aligned reads displayed.

AGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568367‑4568643
                                                                                                                                          |                                                                                                                                             
agGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTaa                                                                                                                                             <  2:439989/140‑1 (MQ=255)
 ggggCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaa                                                                                                                                            >  1:63096/1‑140 (MQ=255)
     ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTaaaaaaa                                                                                                                                        <  1:661292/140‑1 (MQ=255)
       aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg                                                                                                                                      <  2:1130387/140‑1 (MQ=255)
         aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaaaag                                                                                                                                    >  2:44917/1‑136 (MQ=255)
          cTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑Tgg                                                                                                                                  <  1:1016773/140‑1 (MQ=255)
            gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCa                                                                                                                                <  1:497476/140‑1 (MQ=255)
            gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCa                                                                                                                                <  2:793027/140‑1 (MQ=255)
              cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑AGGCATa                                                                                                                              >  2:821055/1‑140 (MQ=255)
                 cGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATaaca                                                                                                                           <  2:1313234/140‑1 (MQ=255)
                   gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATaacaaa                                                                                                                         >  1:797094/1‑140 (MQ=255)
                   gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑TGGGCTaaaaaa                                                                                                                         >  1:882241/1‑140 (MQ=255)
                   gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑TGGCATaacaaa                                                                                                                         >  1:1264310/1‑140 (MQ=255)
                   gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑AGGGATaacaaa                                                                                                                         >  1:1532314/1‑140 (MQ=255)
                    cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATaacaaac                                                                                                                        <  1:1499161/140‑1 (MQ=255)
                    cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATaacaaaa                                                                                                                        >  1:623459/1‑139 (MQ=255)
                       tAAAATCACGTACCACATTAATGATCCCAGGCTGAATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTGAAAAAAAA‑AGGCCAAAAAaacaac                                                                                                                     >  1:812862/1‑140 (MQ=255)
                         aaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTc                                                                                                                   <  1:257719/140‑1 (MQ=255)
                          aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTcc                                                                                                                  <  2:587579/140‑1 (MQ=255)
                          aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTcc                                                                                                                  <  2:715284/140‑1 (MQ=255)
                          aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTcc                                                                                                                  <  1:611868/140‑1 (MQ=255)
                              aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGGCATAC‑AAAATCCACGCCATCGGGAAACACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑AGGCGGAACAAACAAccccctac                                                                                                              >  2:201105/1‑136 (MQ=255)
                               cGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAACAACCCCCT‑GTc                                                                                                            >  2:578805/1‑140 (MQ=255)
                                 tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCg                                                                                                          <  1:1271148/140‑1 (MQ=255)
                                 tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCg                                                                                                          <  1:1522507/140‑1 (MQ=255)
                                  aCCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCgg                                                                                                         <  2:207839/140‑1 (MQ=255)
                                      caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑AGGCATAACAAAAAACTCCCT‑GTCCGGAGaa                                                                                                     >  2:1283046/1‑140 (MQ=255)
                                        ttAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAACAACTCCCT‑GTCCGGAGAAcc                                                                                                   >  1:785831/1‑140 (MQ=255)
                                          aaTGATCCCAGGCTGGATCGGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑AGGCATAACAAACAACTCCCT‑GTCCCGAGAAaccc                                                                                                 >  1:936645/1‑140 (MQ=255)
                                          aaTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAAcccc                                                                                                 <  1:919378/140‑1 (MQ=255)
                                           aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑AGGGAAAACAAACAACCCCTT‑GTCCGGAGAAACCCa                                                                                                >  1:1041968/1‑140 (MQ=255)
                                           aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACA‑TGGCGTAAACAAAAACACCTTAGTCC‑GAGAACCCCa                                                                                                >  1:1341463/1‑140 (MQ=255)
                                              aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑GGGCATAAAAAACAACTCCTT‑GTCCGGAGAACCCCAACg                                                                                             >  1:1267264/1‑140 (MQ=255)
                                              aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑GGGCATAAAAAACAACTCCTT‑TTCCGGGGAACCCCCACg                                                                                             >  1:677908/1‑140 (MQ=255)
                                                cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑GGGGAAAACAAAAAAATCCCT‑GTCCGGGGGACCCCCAAggg                                                                                           >  2:808378/1‑140 (MQ=255)
                                                 ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAAAAACCCCTT‑GTCCGGAGAACCCCAACgggg                                                                                          >  1:1081589/1‑140 (MQ=255)
                                                 ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAAAAAACAACTCCTT‑GTCCGGAGAACCCCAACgggg                                                                                          >  1:188102/1‑140 (MQ=255)
                                                  cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑GGGGATAACAAACAAACCCCT‑GTCCGGAGAAACCCAAAggggg                                                                                         >  1:480837/1‑139 (MQ=255)
                                                   aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAC‑TGGGATAACAAACAAACCCTT‑GTCCCGAGGACCCCCACggggga                                                                                        >  2:535267/1‑138 (MQ=255)
                                                   aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑TGGCATAAAAAACAACTCCCT‑GTCCCGGGAACCCCCACggggga                                                                                        >  2:1375570/1‑138 (MQ=255)
                                                   aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAACAACTCCCT‑GTCCGGGGAAACCCAACGGGGaa                                                                                        >  2:1099963/1‑140 (MQ=255)
                                                     gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑AGGCATAACAAACAACCCCTT‑GTCCGGAGAACCCCCACGGGGgaaa                                                                                      >  1:854263/1‑140 (MQ=255)
                                                         gATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑AGGGACAACAAACAAATCCCT‑GTCCGGAGGACCCCCACGGGGGAAACGaa                                                                                  >  2:918847/1‑140 (MQ=255)
                                                         gATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAA‑AGGCATAAAAAAAAACTCCCT‑GTCCGGAGAAACCCCACGGGGGAAAAGaa                                                                                  >  2:301500/1‑140 (MQ=255)
                                                         gATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑AGGGATAACAAAAAACCCCTT‑TTCCGGACCACCCCAACCGGGGAAACGaa                                                                                  >  2:102429/1‑140 (MQ=255)
                                                         gATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAACG‑CGGGATAAAAAAAAACTCCCT‑GTCCGGGGAACCCCCACGGGGGAAAAGaa                                                                                  >  1:77403/1‑140 (MQ=255)
                                                          aTCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTTAAAAAACG‑CGGGATAAAAAAAAAACCCCT‑GTTCCGGGAAACCCCACGGGGGAAAAGaaa                                                                                 >  1:1540993/1‑140 (MQ=255)
                                                          aTCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑GGGCATAACAAACAACCCCTT‑GTCCGGAGAAACCCCACGGGGGAAAAGaaa                                                                                 >  1:775792/1‑140 (MQ=255)
                                                          aTCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACC‑ACGGCTAAAAAAAAAATCCTT‑TGCCGGGGGACCCCCACGGGGGGAACCaaa                                                                                 >  2:1320056/1‑140 (MQ=255)
                                                          aTCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCCTAAAAAACAACCCCCT‑GTCCGGGGAAACCCCACCGGGGAAACGaaa                                                                                 >  2:756596/1‑140 (MQ=255)
                                                          aTCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑GGGCATAACAAACAACTCCTT‑TTCCGGAGAACCCCCACGGGGGAAACGaaa                                                                                 >  1:705203/1‑140 (MQ=255)
                                                              gATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCa                                                                             <  2:797094/140‑1 (MQ=255)
                                                              gATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAA‑AGGGAAAAAAAAAAACCCCCT‑TTCCGGGGAACCCCCACGGGGGAAACGAAAATCa                                                                             >  2:1452432/1‑140 (MQ=255)
                                                               aTCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑TGGCATAACAAACAACTCCCT‑GTCCGGAGAACCCCCAAGGGGGAAAAGAAAATCaa                                                                            >  1:1037592/1‑140 (MQ=255)
                                                                   gTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  1:825844/140‑1 (MQ=255)
                                                                   gTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  2:513279/140‑1 (MQ=255)
                                                                    tCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑GGGCATAAAAAAAAAATCCTT‑GTCCGGCGGACCCCAACGGGGGAAACGAAAATCAATACCg                                                                       >  2:740849/1‑140 (MQ=255)
                                                                    tCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACG‑AGGCATAACAAAAAAACCCTT‑GTCCGGAGAAACCCAACGGGGGAAACGAAAATCAAAAccc                                                                       >  2:1327400/1‑139 (MQ=255)
                                                                    tCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAAAAACCCCCT‑GTCCCGAGGACCCCCACCGGGGAAAAGAAAATCAAAAccc                                                                       >  2:301521/1‑139 (MQ=255)
                                                                    tCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAAAAAACAACTCCTT‑TTCCGGAGAAACCCAACGGGGAAAAAGAAAATCAATAccc                                                                       >  1:288873/1‑139 (MQ=255)
                                                                     cGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGt                                                                      <  1:650895/140‑1 (MQ=255)
                                                                     cGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAAAAACTCCCT‑GTCCGGAGAACCCCCACGGGGGAAAAGAAAATCAATACCGt                                                                      >  1:487050/1‑140 (MQ=255)
                                                                         aGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑GGGCATAAAAAACAACCCCCT‑GTCCGGGGAACCCCCACGGGGGAAACGAAAATCAAAAACGTCggg                                                                  >  2:196483/1‑139 (MQ=255)
                                                                           aTACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                                <  1:44917/140‑1 (MQ=255)
                                                                           aTACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                                <  2:831883/140‑1 (MQ=255)
                                                                             aCGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑GGGCATAAAAAACAAATCCTT‑GTCCGGGGAACCCCCACGGGGGAAAAGAAAATCAAAAACGTCGGTAATg                                                              >  2:1247610/1‑140 (MQ=255)
                                                                              cGCAGCAAAGTCATAC‑AAAATCAACGCCATCAGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAG‑TGGCATAAAAAAAAAATCCTT‑GTCCGCAGAACCCCAACCGGGGAAAAGAAAATCAAAACCCTCGGTAATGa                                                             >  2:321374/1‑140 (MQ=255)
                                                                                cagcaAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg                                                           <  1:1163093/140‑1 (MQ=255)
                                                                                 agcaAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGa                                                          <  2:1395495/140‑1 (MQ=255)
                                                                                   caAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  1:201105/140‑1 (MQ=255)
                                                                                   caAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAACAAACAAATCCTT‑GTCCGGAGAACCCCCACGGGGGAAAAGAAAATCAATACCCTCGGGAATGACGaaa                                                        >  2:713887/1‑140 (MQ=255)
                                                                                    caaGTCAAACAAAAATCAACGCCTCCGGGAAAACCACGGGGGCGCCATGGCGGTTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  2:1452434/139‑1 (MQ=255)
                                                                                      aGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGCGGGGAAAAAAAAAACCCCCTT‑TTCCGG‑GAACCCCCACCGGGGAAACGAAAATCAAAACCATCGGGAATGACGaaaagg                                                     >  2:679055/1‑137 (MQ=255)
                                                                                         cATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCCT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAgg                                                  >  1:1316603/1‑140 (MQ=255)
                                                                                             c‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                              <  2:99734/140‑1 (MQ=255)
                                                                                               aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga                                             <  2:126913/140‑1 (MQ=255)
                                                                                               aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga                                             <  1:1283046/140‑1 (MQ=255)
                                                                                               aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga                                             <  1:398717/140‑1 (MQ=255)
                                                                                                 aaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAG‑TGGCATAAAAAACAACTCCCT‑GTCCGGAGAACCCCCACGGGGGAAAAGAAAATCAATACCCTCGGTAATGACGAAAAGCAGGGGAGAAg                                           >  2:597520/1‑140 (MQ=255)
                                                                                                      aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCaa                                      <  1:1183242/140‑1 (MQ=255)
                                                                                                       cGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc                                     <  2:77403/140‑1 (MQ=255)
                                                                                                        gCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACg                                    <  1:155063/140‑1 (MQ=255)
                                                                                                         ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                   <  1:55345/140‑1 (MQ=255)
                                                                                                         ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                   <  1:522104/140‑1 (MQ=255)
                                                                                                                 aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  2:838407/140‑1 (MQ=255)
                                                                                                                 aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  2:1267264/140‑1 (MQ=255)
                                                                                                                 aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  2:1264310/140‑1 (MQ=255)
                                                                                                                 aaaaaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  1:1320056/140‑1 (MQ=255)
                                                                                                                   aaaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAAt                         <  1:1062385/140‑1 (MQ=255)
                                                                                                                    aaCAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATg                        <  1:944129/140‑1 (MQ=255)
                                                                                                                         cGTGGCGGCATGGCGGGTAAAAAAAAA‑GGGCATAAAAAAAAACTCCTT‑GTTCGGAGAACCCCAACGGGGAAAAAGAAATTCAATAACGTCGGTAATGACGAAAAGCAGGGGGGAAGGGGAACGAGAAAGCaaaaagacca                   >  2:408240/1‑133 (MQ=255)
                                                                                                                           tGGCGGCATGGCGGGTAAAAAAACG‑CGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGaa                 <  2:1173451/140‑1 (MQ=255)
                                                                                                                             gcggcATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAAtt               <  1:1452432/140‑1 (MQ=255)
                                                                                                                               ggcATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTc             <  2:1532314/140‑1 (MQ=255)
                                                                                                                                    ggCGGGTAAAAAAACG‑TGGTATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:1160206/140‑1 (MQ=255)
                                                                                                                                    ggCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  2:705203/140‑1 (MQ=255)
                                                                                                                                    ggCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  2:936645/140‑1 (MQ=255)
                                                                                                                                        ggTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCa    <  1:196483/140‑1 (MQ=255)
                                                                                                                                        ggTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCa    <  2:1143698/140‑1 (MQ=255)
                                                                                                                                        ggTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCa    <  2:480837/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                             
AGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATAC‑AAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACG‑TGGCATAACAAACAACTCCTT‑GTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568367‑4568643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑