Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,273,000	T→C	Y213H (TAC→CAC)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,273,000	0	T	C	100.0%	254.9 / NA	70	Y213H (TAC→CAC)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/35);  total (35/35)

CGTGCGCATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCGATG  >  NC_000913/2272864‑2273132                                                                                                                                         |                                                                                                                                     cGTGCGCATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCAcc                                                                                                                                   >  2:1378009/1‑140 (MQ=255)   tGCGCATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACcgc                                                                                                                                 >  1:1371905/1‑140 (MQ=255)    gcgcATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACcgcg                                                                                                                                >  2:1082894/1‑140 (MQ=255)    gcgcATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACcgcg                                                                                                                                >  1:345764/1‑140 (MQ=255)      gcATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGtt                                                                                                                              >  2:1298421/1‑140 (MQ=255)      gcATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGtt                                                                                                                              <  1:792520/140‑1 (MQ=255)         ttGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACg                                                                                                                           <  2:783289/140‑1 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  1:1301161/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:191307/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:1095712/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:15371/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  1:934345/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:318224/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  1:701283/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  1:484321/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:615049/1‑140 (MQ=255)                      aCCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATgg                                                                                                              >  2:1429325/1‑140 (MQ=255)                         cGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGc                                                                                                           <  2:988351/140‑1 (MQ=255)                         cGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGc                                                                                                           <  1:412430/140‑1 (MQ=255)                         cGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGc                                                                                                           <  1:1319335/140‑1 (MQ=255)                          ggCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCa                                                                                                          <  1:1378009/140‑1 (MQ=255)                           gCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCaa                                                                                                         >  2:942527/1‑140 (MQ=255)                           gCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCaa                                                                                                         >  2:22660/1‑140 (MQ=255)                               agaagaTATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGATGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAata                                                                                                     <  1:768931/140‑1 (MQ=255)                               agaagaTATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAata                                                                                                     <  1:247205/140‑1 (MQ=255)                               agaagaTATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAata                                                                                                     <  2:387807/140‑1 (MQ=255)                                        gCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTAtt                                                                                            >  1:409007/1‑140 (MQ=255)                                         cTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTc                                                                                           <  1:159769/140‑1 (MQ=255)                                         cTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTc                                                                                           <  1:834570/140‑1 (MQ=255)                                           gAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTccc                                                                                         >  2:108210/1‑140 (MQ=255)                                           gAGTCTGTTTTCGCTCCAGGGATTTCGAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCCATCGCCACGCCTCCGCTTGCCTGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTccc                                                                                         >  2:382263/1‑140 (MQ=255)                                            aGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCg                                                                                        >  2:1237623/1‑140 (MQ=255)                                              tCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCgtg                                                                                      >  1:856647/1‑140 (MQ=255)                                              tCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCgtg                                                                                      <  2:820012/140‑1 (MQ=255)                                                  ttttCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGaa                                                                                  >  1:1340854/1‑140 (MQ=255)                                                   tttCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGaaa                                                                                 <  2:896661/140‑1 (MQ=255)                                                   tttCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCAGTGTGaaa                                                                                 <  2:913476/140‑1 (MQ=255)                                                                  ttCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGgcagca                                                                  <  1:789535/140‑1 (MQ=255)                                                                  ttCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGgcagca                                                                  <  2:699376/140‑1 (MQ=255)                                                                    ccAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCaaa                                                                <  1:1095712/140‑1 (MQ=255)                                                                    ccAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCaaa                                                                <  1:1180060/140‑1 (MQ=255)                                                                     cAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAAc                                                               >  2:1506501/1‑140 (MQ=255)                                                                      aGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACt                                                              <  1:318224/140‑1 (MQ=255)                                                                       gAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACtt                                                             <  1:615049/140‑1 (MQ=255)                                                                        aaaaGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTa                                                            <  2:165204/140‑1 (MQ=255)                                                                        aaaaGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTa                                                            <  1:15371/140‑1 (MQ=255)                                                                         aaaGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTAc                                                           >  1:177016/1‑140 (MQ=255)                                                                             tACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGt                                                       <  1:71278/140‑1 (MQ=255)                                                                             tACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGt                                                       <  1:1429325/140‑1 (MQ=255)                                                                                     ggATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTg                                               <  1:1082894/140‑1 (MQ=255)                                                                                       aTCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGa                                             <  2:1414350/140‑1 (MQ=255)                                                                                                ttAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAAt                                    >  2:90656/1‑140 (MQ=255)                                                                                                 tAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAAtt                                   >  1:416208/1‑140 (MQ=255)                                                                                                 tAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAAtt                                   >  1:870140/1‑140 (MQ=255)                                                                                                  aGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAAttt                                  >  1:1306435/1‑140 (MQ=255)                                                                                                       cccGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGaca                             <  2:1306435/140‑1 (MQ=255)                                                                                                         cGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACAcc                           <  1:108210/140‑1 (MQ=255)                                                                                                         cGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACAcc                           <  2:934345/140‑1 (MQ=255)                                                                                                          gCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCa                          <  2:1209492/140‑1 (MQ=255)                                                                                                          gCTCGCCACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCa                          <  1:22660/140‑1 (MQ=255)                                                                                                                cACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCt                    <  1:856816/140‑1 (MQ=255)                                                                                                                cACGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCt                    <  1:1506501/140‑1 (MQ=255)                                                                                                                 aCGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTa                   <  2:1371905/140‑1 (MQ=255)                                                                                                                  cGCCTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTAc                  <  1:461837/140‑1 (MQ=255)                                                                                                                     cTCCGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGat               >  1:140476/1‑140 (MQ=255)                                                                                                                        cGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGattat            >  1:1540209/1‑140 (MQ=255)                                                                                                                        cGCTTGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGattat            >  2:903517/1‑140 (MQ=255)                                                                                                                            tGCCAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTAcc        <  2:817719/140‑1 (MQ=255)                                                                                                                              ccAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTc      >  1:401193/1‑140 (MQ=255)                                                                                                                               cAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCg     >  2:1341005/1‑140 (MQ=255)                                                                                                                               cAGTGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCg     <  2:969324/140‑1 (MQ=255)                                                                                                                                  tGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCgatg  >  2:1342419/1‑140 (MQ=255)                                                                                                                                  tGGTCCGCACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCgatg  >  2:1418242/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTGCGCATTGAGTTAGCTAAACCCGGCAAAGAAGATATGCTGAGTCTGTTTTCGCTGCCGGTATTTCCAGAAAAGTACTGGAAGGATCACAAACTTAGCGACCCGCTCGCCACGCCTCCGCTTGCCAGTGGTCCGTACCGCGTTACGTCCTGGAAAATGGGGCAAAATATTGTCTATTCCCGTGTGAAAGATTACTGGGCAGCAAACTTACCGGTAAACCGTGGACGCTGGAATTTCGACACCATTCGCTACGATTATTACCTCGATG  >  NC_000913/2272864‑2273132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑