Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,596	T→C	V63A (GTT→GCT)	cysK →	O‑acetylserine sulfhydrylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,596	0	T	C	100.0%	329.1 / NA	88	V63A (GTT→GCT)	cysK	O‑acetylserine sulfhydrylase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/56);  total (32/56)

GTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGG  >  NC_000913/2532460‑2532731                                                                                                                                         |                                                                                                                                        gTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGa                                                                                                                                      <  2:1000284/140‑1 (MQ=255)  ttCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGaa                                                                                                                                     >  2:1451225/1‑140 (MQ=255)   tCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAAc                                                                                                                                    <  2:170049/140‑1 (MQ=255)      ccTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTgg                                                                                                                                 >  1:1048702/1‑140 (MQ=255)      ccTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTgg                                                                                                                                 >  1:246915/1‑140 (MQ=255)      ccTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTgg                                                                                                                                 >  2:876407/1‑140 (MQ=255)      ccTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTgg                                                                                                                                 >  1:440690/1‑140 (MQ=255)           aTCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGaa                                                                                                                            <  2:123172/140‑1 (MQ=255)            tCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGTCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAAc                                                                                                                           <  2:941769/140‑1 (MQ=255)              gCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCg                                                                                                                         <  1:796672/140‑1 (MQ=255)               cATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGa                                                                                                                        <  2:988111/140‑1 (MQ=255)                 ttggTAATGGACGCATTCTGGCGAAGGTGGAATTACGTATCCCCAGCTTCTTCGTTAGGTGCTGTATGGGTGCCAGCTTGATTTTGGATGCAGAATAGCTCAGTGTGCTGAAACCAGGAGCTGAACTAGTTTAACCGTcc                                                                                                                      <  2:1364204/138‑1 (MQ=255)                 tCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGAcc                                                                                                                      <  2:18463/140‑1 (MQ=255)                   ggTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAg                                                                                                                    <  2:413791/140‑1 (MQ=255)                     tAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCg                                                                                                                  <  1:576613/140‑1 (MQ=255)                     tAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCg                                                                                                                  >  2:440237/1‑140 (MQ=255)                     tAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCg                                                                                                                  <  2:369906/140‑1 (MQ=255)                       aCGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGt                                                                                                                <  1:1322401/140‑1 (MQ=255)                          gACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAAt                                                                                                             <  1:1427546/140‑1 (MQ=255)                            cGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATAc                                                                                                           <  1:1541767/140‑1 (MQ=255)                               aTTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCgg                                                                                                        >  1:1222663/1‑140 (MQ=255)                               aTTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCgg                                                                                                        >  2:432536/1‑140 (MQ=255)                                 tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                                                                                      <  2:18768/140‑1 (MQ=255)                                 tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                                                                                      <  1:266125/140‑1 (MQ=255)                                 tCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGa                                                                                                      <  1:132758/140‑1 (MQ=255)                                     gCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGc                                                                                                  <  2:309118/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  1:726738/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  2:630347/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  2:1218188/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  1:1293855/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  2:513952/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  1:752856/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  2:440690/140‑1 (MQ=255)                                      cGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCa                                                                                                 <  1:197428/140‑1 (MQ=255)                                        aaGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACt                                                                                               <  2:259420/140‑1 (MQ=255)                                          ggTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTTCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTgg                                                                                             <  2:1165371/140‑1 (MQ=255)                                          ggTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTgg                                                                                             >  2:467076/1‑140 (MQ=255)                                           gTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGc                                                                                            >  2:479713/1‑140 (MQ=255)                                           gTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGc                                                                                            <  1:956902/140‑1 (MQ=255)                                                 tctcGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGt                                                                                      >  1:1373843/1‑140 (MQ=255)                                                           ccAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCt                                                                            <  2:505069/140‑1 (MQ=255)                                                             aGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTcg                                                                          <  2:1066351/140‑1 (MQ=255)                                                             aGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTcg                                                                          <  2:1048702/140‑1 (MQ=255)                                                             aGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTcg                                                                          <  1:440237/140‑1 (MQ=255)                                                               cTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTcgcg                                                                        >  2:922689/1‑140 (MQ=255)                                                                  cAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGtt                                                                     <  2:246915/140‑1 (MQ=255)                                                                   aGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTa                                                                    <  1:175263/140‑1 (MQ=255)                                                                   aGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTa                                                                    >  2:1412503/1‑140 (MQ=255)                                                                    gCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTAc                                                                   >  1:1446110/1‑140 (MQ=255)                                                                       ttAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACaaa                                                                <  2:210742/140‑1 (MQ=255)                                                                       ttAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACaaa                                                                <  2:155746/140‑1 (MQ=255)                                                                        tAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAAc                                                               <  1:1019680/140‑1 (MQ=255)                                                                        tAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAAc                                                               <  2:710142/140‑1 (MQ=255)                                                                        tAAGAGCCGTTGCTGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTTAACCGGCCATCGGTAATTCCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAAc                                                               <  2:1028541/140‑1 (MQ=255)                                                                         aaGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACt                                                              <  1:1500374/140‑1 (MQ=255)                                                                              ccGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCAccc                                                         >  2:1066298/1‑140 (MQ=255)                                                                                 tattggTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGa                                                      >  1:1266813/5‑140 (MQ=255)                                                                                 tATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGa                                                      >  2:69872/1‑140 (MQ=255)                                                                                 tATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGa                                                      >  1:1297888/1‑140 (MQ=255)                                                                                  aTCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGAc                                                     <  1:1451225/140‑1 (MQ=255)                                                                                   tCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGAcc                                                    <  1:1235109/140‑1 (MQ=255)                                                                                     ggTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGGCCAt                                                  <  2:1222663/140‑1 (MQ=255)                                                                                     ggTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCAt                                                  <  1:479713/140‑1 (MQ=255)                                                                                     ggTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCAt                                                  >  2:410229/1‑140 (MQ=255)                                                                                      gTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATg                                                 >  1:407538/1‑140 (MQ=255)                                                                                      gTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATg                                                 >  1:277275/1‑140 (MQ=255)                                                                                       tGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGc                                                <  2:1047405/140‑1 (MQ=255)                                                                                        gCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGcc                                               >  1:1008219/1‑140 (MQ=255)                                                                                        gCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGcc                                               >  1:83472/1‑140 (MQ=255)                                                                                         ccAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACGGGTTGACCCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCa                                              <  2:790901/140‑1 (MQ=255)                                                                                          cAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAg                                             <  1:288575/140‑1 (MQ=255)                                                                                             cATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGaaa                                          >  1:870506/1‑140 (MQ=255)                                                                                               tGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAAcc                                        <  2:124756/140‑1 (MQ=255)                                                                                                 aTTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCAt                                      <  1:342610/140‑1 (MQ=255)                                                                                                   ttGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGCCCAGCGGTAATACCGGGATTGCACCGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGa                                    >  2:89928/1‑140 (MQ=255)                                                                                                   ttGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGa                                    <  2:1077256/140‑1 (MQ=255)                                                                                                         tGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTg                              >  2:32901/1‑140 (MQ=255)                                                                                                          gCCGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGa                             <  2:925881/140‑1 (MQ=255)                                                                                                           ccGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGaa                            <  2:1446110/140‑1 (MQ=255)                                                                                                           ccGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGaa                            >  2:558391/1‑140 (MQ=255)                                                                                                           ccGAAAAGCGCGGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGaa                            >  2:1245701/1‑140 (MQ=255)                                                                                                             gAAAAGCGCGGCGTGCTG‑AACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCAACCTGACCATGCCAGAAACCATGAGTATTGAAcgc                         >  1:966826/1‑140 (MQ=255)                                                                                                                   cgcgGCGTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCaa                    <  2:1297888/140‑1 (MQ=255)                                                                                                                     cggcggGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAgc                  >  1:649510/1‑140 (MQ=255)                                                                                                                     cggcgTGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAgc                  >  2:641271/1‑140 (MQ=255)                                                                                                                          tGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGctgctg             <  1:1201933/140‑1 (MQ=255)                                                                                                                          tGCTGAAACCAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGctgctg             <  1:32901/140‑1 (MQ=255)                                                                                                                                  ccAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGtt     <  2:1261686/140‑1 (MQ=255)                                                                                                                                  ccAGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGtt     <  1:467076/140‑1 (MQ=255)                                                                                                                                    aGGCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAg   >  1:1339906/1‑140 (MQ=255)                                                                                                                                     ggCGCTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAgg  >  2:180196/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTTCGCCTGAATCGCATCGGTAACGGACGCATTCTGGCGAAGGTGGAATCTCGTAACCCCAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAACATGATTTGGGATGCCGAAAAGCGCGGCGTGCTGAAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGG  >  NC_000913/2532460‑2532731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑