Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,536,991	G→A	R83C (CGT→TGT)	pdxK ←	pyridoxal kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,536,991	0	G	A	98.8%	281.2 / NA	86	R83C (CGT→TGT)	pdxK	pyridoxal kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (51/34);  minor base C (0/1);  total (51/35)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATA  >  NC_000913/2536855‑2537124                                                                                                                                         |                                                                                                                                      aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:761567/1‑140 (MQ=255) aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:442883/1‑140 (MQ=255) aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  2:432533/1‑140 (MQ=255) aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  1:46829/1‑140 (MQ=255) aTCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTc                                                                                                                                    >  1:815900/1‑140 (MQ=255)    aCCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTg                                                                                                                                 <  2:1329306/140‑1 (MQ=255)     ccGGATCGACCATGTTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGa                                                                                                                                >  1:88976/1‑140 (MQ=255)       ggATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAg                                                                                                                              <  1:323402/140‑1 (MQ=255)        gATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAgc                                                                                                                             >  1:17346/1‑140 (MQ=255)        gATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGCTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAgc                                                                                                                             >  1:464679/1‑140 (MQ=255)           cGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgca                                                                                                                          <  1:1230183/140‑1 (MQ=255)            gACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcac                                                                                                                         >  1:797272/1‑140 (MQ=255)             aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        <  2:1393366/140‑1 (MQ=255)             aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        >  1:1127215/1‑140 (MQ=255)             aCCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGcgcacg                                                                                                                        >  2:526407/1‑140 (MQ=255)                   aTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATa                                                                                                                  >  2:334412/1‑140 (MQ=255)                   aTCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATa                                                                                                                  >  2:1273546/1‑140 (MQ=255)                        tAGGTCAGGATGGTCTTTGCGTAGCGCGGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGc                                                                                                             >  2:847981/1‑140 (MQ=255)                        tAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGc                                                                                                             >  1:852339/1‑140 (MQ=255)                             cAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAAc                                                                                                        >  1:487749/1‑140 (MQ=255)                               ggATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGTCGCTAAACCa                                                                                                      >  2:1195772/1‑140 (MQ=255)                                gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     <  2:514073/140‑1 (MQ=255)                                gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     >  1:997022/1‑140 (MQ=255)                                gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     >  1:571178/1‑140 (MQ=255)                                gATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTCGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCAt                                                                                                     <  2:1251439/140‑1 (MQ=255)                                     tCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTc                                                                                                >  1:1112319/1‑140 (MQ=255)                                     tCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTc                                                                                                >  1:804586/1‑140 (MQ=255)                                          gCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGaa                                                                                           >  2:75011/1‑140 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:424512/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:1077160/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:99835/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:84376/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:1232858/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:128139/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  1:174662/140‑1 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      >  2:615937/1‑140 (MQ=255)                                               gcgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:46829/140‑1 (MQ=255)                                                cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     <  2:350463/140‑1 (MQ=255)                                                cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     >  1:543206/1‑140 (MQ=255)                                                cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac                                                                                     <  2:88976/140‑1 (MQ=255)                                                cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      <  2:337877/139‑1 (MQ=255)                                                cgcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca                                                                                      >  1:337877/1‑139 (MQ=255)                                                 gcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcacc                                                                                    <  1:243099/140‑1 (MQ=255)                                                 gcAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCAGAATCGcacc                                                                                    <  1:542711/140‑1 (MQ=255)                                                   aGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac                                                                                  >  1:838522/1‑140 (MQ=255)                                                    gTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCaccacc                                                                                 <  1:432533/140‑1 (MQ=255)                                                     tCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCg                                                                                >  1:899125/1‑140 (MQ=255)                                                       aGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTa                                                                              <  2:258177/140‑1 (MQ=255)                                                         ccACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGa                                                                            <  2:78605/140‑1 (MQ=255)                                                          cACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa                                                                           >  1:1108736/1‑140 (MQ=255)                                                             tCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAgt                                                                        >  1:101821/1‑140 (MQ=255)                                                                     gATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAAt                                                                >  1:409492/1‑140 (MQ=255)                                                                      aTTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg                                                               >  2:1142703/1‑140 (MQ=255)                                                                      aTTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg                                                               <  1:761567/140‑1 (MQ=255)                                                                        tttGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcg                                                             <  1:75011/140‑1 (MQ=255)                                                                              ttGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTAt                                                       <  2:674451/140‑1 (MQ=255)                                                                                  gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  2:1246637/140‑1 (MQ=255)                                                                                  gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  2:804586/140‑1 (MQ=255)                                                                                  gATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCt                                                   <  1:334412/140‑1 (MQ=255)                                                                                       cGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCa                                              <  1:615937/140‑1 (MQ=255)                                                                                         ttCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAAt                                            >  1:36203/1‑140 (MQ=255)                                                                                              atatAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGt                                       <  2:852339/140‑1 (MQ=255)                                                                                              atatAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGt                                       >  2:1044479/1‑140 (MQ=255)                                                                                                ataGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcg                                     >  2:455241/1‑140 (MQ=255)                                                                                                ataGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcg                                     >  1:1415647/1‑140 (MQ=255)                                                                                                 taGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcgg                                    <  1:1195772/140‑1 (MQ=255)                                                                                                  aGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggc                                   <  1:694536/140‑1 (MQ=255)                                                                                                   gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  >  2:309862/1‑140 (MQ=255)                                                                                                   gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  <  2:769480/140‑1 (MQ=255)                                                                                                   gCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggca                                  >  2:223582/1‑140 (MQ=255)                                                                                                    cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  2:1362490/1‑140 (MQ=255)                                                                                                    cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:437357/1‑140 (MQ=255)                                                                                                    cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:34062/1‑140 (MQ=255)                                                                                                    cccGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGGCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  2:1359983/1‑140 (MQ=255)                                                                                                    cccGTGGTTACAGCACGAAGTTGGCGCAGCACATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcac                                 >  1:736586/1‑140 (MQ=255)                                                                                                      cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  2:601215/1‑140 (MQ=255)                                                                                                      cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  1:1302756/1‑140 (MQ=255)                                                                                                      cGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTcggcacgg                               >  2:1280967/1‑140 (MQ=255)                                                                                                                 cACGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTc                    >  2:1113414/1‑140 (MQ=255)                                                                                                                   cGAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAg                  >  2:1269286/1‑140 (MQ=255)                                                                                                                    gAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAgg                 <  1:1142703/140‑1 (MQ=255)                                                                                                                    gAAGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCCTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAgg                 >  2:1515893/1‑140 (MQ=255)                                                                                                                     aaGTTGGCGCAGCGCATCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGc                >  2:663690/1‑140 (MQ=255)                                                                                                                           gcgcagcgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCt          >  1:115545/1‑140 (MQ=255)                                                                                                                                gcgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTg     >  1:299250/1‑140 (MQ=255)                                                                                                                                 cgcaTCACCCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGa    <  2:1127215/140‑1 (MQ=255)                                                                                                                                 cgcaTCACACTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGa    >  1:579515/1‑140 (MQ=255)                                                                                                                                   caTCACCCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATa  <  2:997022/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ATCACCGGATCGACCATGATCAATAGGTCAGGATGGTCTTTGCGTAGCGCAGTCAGCCACTCGGCAAGGATTTTGATTTGCGATGCCGTTCCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATA  >  NC_000913/2536855‑2537124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑