Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,546,759	T→C	C29C (TGT→TGC)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,546,759	0	T	C	100.0%	241.0 / NA	64	C29C (TGT→TGC)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/33);  total (31/33)

GGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCG  >  NC_000913/2546624‑2546893                                                                                                                                        |                                                                                                                                       ggATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGa                                                                                                                                    <  1:288380/140‑1 (MQ=255)  gATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGAc                                                                                                                                   <  1:1127045/140‑1 (MQ=255)      aaCACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACgcgc                                                                                                                               <  1:647994/140‑1 (MQ=255)      aaCACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACgcgc                                                                                                                               <  2:91265/140‑1 (MQ=255)            gcggcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGt                                                                                                                         <  2:629971/140‑1 (MQ=255)             cggcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTc                                                                                                                        >  2:997148/1‑140 (MQ=255)                cTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTgg                                                                                                                     <  1:712209/140‑1 (MQ=255)                cTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTgg                                                                                                                     <  1:1289342/140‑1 (MQ=255)                cTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTgg                                                                                                                     <  1:1105874/140‑1 (MQ=255)                  ttATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGgt                                                                                                                   <  1:167389/140‑1 (MQ=255)                     ttCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTa                                                                                                                <  2:1255579/140‑1 (MQ=255)                         tCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATg                                                                                                            >  2:1013074/1‑140 (MQ=255)                           aGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGAc                                                                                                          <  2:1521373/140‑1 (MQ=255)                             gTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAg                                                                                                        <  2:906466/140‑1 (MQ=255)                               tttAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGtt                                                                                                      <  1:1035309/140‑1 (MQ=255)                                       aGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGtt                                                                                              <  2:673217/140‑1 (MQ=255)                                       aGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGtt                                                                                              <  1:1228245/140‑1 (MQ=255)                                        ggAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTg                                                                                             <  2:1487439/140‑1 (MQ=255)                                        ggAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTg                                                                                             <  1:1248937/140‑1 (MQ=255)                                             aaCCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATccc                                                                                        <  1:367557/140‑1 (MQ=255)                                             aaCCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATccc                                                                                        >  1:943404/1‑140 (MQ=255)                                                   tGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATc                                                                                  >  2:1317041/1‑140 (MQ=255)                                                   tGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATc                                                                                  >  1:745077/1‑140 (MQ=255)                                                    ggCCAAAGGAAACAGCAGGGAACCTCTGAACCCCATTTTTTCCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCa                                                                                 <  2:889961/140‑1 (MQ=255)                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTGCCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCa                                                                                 >  1:209270/1‑140 (MQ=255)                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCa                                                                                 >  1:1497006/1‑140 (MQ=255)                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCa                                                                                 >  2:1492063/1‑140 (MQ=255)                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCa                                                                                 >  2:696214/1‑140 (MQ=255)                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTG‑GGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCaa                                                                                >  1:69969/1‑140 (MQ=255)                                                      ccAAAGAGATCAGCAGTGAACTTCTGATCACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCaaa                                                                               <  1:750936/140‑1 (MQ=255)                                                      ccAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCaaa                                                                               <  1:335406/140‑1 (MQ=255)                                                          agagATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGc                                                                           >  2:1157094/1‑140 (MQ=255)                                                             gaTCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCCGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTgg                                                                        <  1:1212862/140‑1 (MQ=255)                                                              aTCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGa                                                                       <  1:1013074/140‑1 (MQ=255)                                                               tCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGaa                                                                      >  1:1150200/1‑140 (MQ=255)                                                               tCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGaa                                                                      <  1:126487/140‑1 (MQ=255)                                                                   cagTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCg                                                                  >  2:1473132/1‑140 (MQ=255)                                                                      tGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGa                                                               >  1:397096/1‑140 (MQ=255)                                                                      tGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGa                                                               >  1:1290835/1‑140 (MQ=255)                                                                        aaCTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAg                                                             >  2:1350181/1‑140 (MQ=255)                                                                         aCTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAgg                                                            >  1:1407391/1‑140 (MQ=255)                                                                            tCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCg                                                         <  1:592302/140‑1 (MQ=255)                                                                                      aTTCTTGCCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGAc                                               <  1:875752/140‑1 (MQ=255)                                                                                      aTTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGAc                                               <  1:696214/140‑1 (MQ=255)                                                                                       ttCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGAcc                                              >  1:55661/1‑140 (MQ=255)                                                                                         cTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAg                                            <  1:1009382/140‑1 (MQ=255)                                                                                         cTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAg                                            <  1:1317041/140‑1 (MQ=255)                                                                                               cGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCa                                      >  1:967089/1‑140 (MQ=255)                                                                                                gtgtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAg                                     >  2:1160421/1‑140 (MQ=255)                                                                                                 tgtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAgg                                    >  2:497255/1‑140 (MQ=255)                                                                                                  gtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAggg                                   >  2:1491198/1‑139 (MQ=255)                                                                                                  gtCGGCGGACCGGGAAATATCGCCAGTTGCGGTAACTGCATGACGCGCCTGCGTCTGGGCGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGCGCGTCATTTTGACCAGCGACCAGGt                                   >  2:677656/1‑140 (MQ=255)                                                                                                      gcggACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAg                               >  1:1385999/1‑140 (MQ=255)                                                                                                          aCCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCg                           >  1:148367/1‑140 (MQ=255)                                                                                                            cGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGtt                         >  2:341919/1‑140 (MQ=255)                                                                                                              ggAAATATCGCCCGTTGTGGTATCTGCCTGACGCGCCTGCGTCTGGGTGGACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAACGGCATCATTTTGACCAACGACTAAGTGCAGGTCGtttt                       >  2:1120554/1‑140 (MQ=255)                                                                                                                 aaTATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTgg                    <  1:1157094/140‑1 (MQ=255)                                                                                                                  atatCGCCAGTTGTGGTAGCTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTggg                   <  2:1290835/140‑1 (MQ=255)                                                                                                                   tatCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGc                  <  1:561388/140‑1 (MQ=255)                                                                                                                    atCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGcc                 <  1:365252/140‑1 (MQ=255)                                                                                                                      cGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTg               >  2:1516441/1‑140 (MQ=255)                                                                                                                        ccAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGt             >  1:329283/1‑140 (MQ=255)                                                                                                                        ccAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGt             >  1:781015/1‑140 (MQ=255)                                                                                                                         cAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTa            >  1:1295523/1‑140 (MQ=255)                                                                                                                              gtgGTAACTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAgcg       >  2:950144/1‑140 (MQ=255)                                                                                                                                   aaCTGCATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCg  <  1:341919/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCG  >  NC_000913/2546624‑2546893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑