Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,588,164	G→A	P190P (CCG→CCA)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,588,164	0	G	A	100.0%	263.4 / NA	75	P190P (CCG→CCA)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/32);  total (43/32)

ACCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCCACCATTAAC  >  NC_000913/2588027‑2588299                                                                                                                                          |                                                                                                                                        aCCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGc                                                                                                                                       >  1:1499295/1‑140 (MQ=255)  ccGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCg                                                                                                                                      >  2:625572/1‑140 (MQ=255)     aTGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCATTATTCCATGCGTATCTGGCTGGACCCAGCGaaa                                                                                                                                   >  1:470728/1‑140 (MQ=255)     aTGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGaaa                                                                                                                                   >  1:1247559/1‑140 (MQ=255)       ggTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACt                                                                                                                                 >  1:112895/1‑140 (MQ=255)          tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCaa                                                                                                                              <  1:596296/140‑1 (MQ=255)          tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCaa                                                                                                                              <  2:884567/140‑1 (MQ=255)          tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCaa                                                                                                                              >  1:333855/1‑140 (MQ=255)            gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACa                                                                                                                            >  1:707851/1‑140 (MQ=255)            gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACa                                                                                                                            >  2:1124362/1‑140 (MQ=255)                   aaaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCa                                                                                                                     >  1:1019575/1‑140 (MQ=255)                      cAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGAt                                                                                                                  <  2:363700/140‑1 (MQ=255)                      cAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGAt                                                                                                                  <  2:773661/140‑1 (MQ=255)                      cAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGAt                                                                                                                  <  1:962923/140‑1 (MQ=255)                      cAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGAt                                                                                                                  <  1:728442/140‑1 (MQ=255)                       aGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCATTATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATg                                                                                                                 <  2:470728/140‑1 (MQ=255)                       aGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATg                                                                                                                 <  2:1403815/140‑1 (MQ=255)                          atatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGAATCTAGCAGGAACCAGCGAAACTCAACAGTTTCCAGATGAcc                                                                                                              >  2:574176/1‑139 (MQ=255)                           tatTGCTGATTATGTTGCCAGTATTATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACgg                                                                                                             >  1:1250962/1‑140 (MQ=255)                           tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACgg                                                                                                             >  2:81076/1‑140 (MQ=255)                              tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTa                                                                                                          >  2:281818/1‑140 (MQ=255)                              tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTa                                                                                                          >  2:262785/1‑140 (MQ=255)                              tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTa                                                                                                          >  1:1009002/1‑140 (MQ=255)                               gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                         <  2:322784/140‑1 (MQ=255)                                   aTTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAt                                                                                                     >  1:1287709/1‑140 (MQ=255)                                   aTTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAt                                                                                                     <  2:196109/140‑1 (MQ=255)                                     tATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGt                                                                                                   >  2:892978/1‑140 (MQ=255)                                     tATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGt                                                                                                   >  1:687859/1‑140 (MQ=255)                                        gTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCAc                                                                                                >  1:828672/1‑140 (MQ=255)                                        gTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCAc                                                                                                <  1:1142970/140‑1 (MQ=255)                                          tGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg                                                                                              >  1:664939/1‑140 (MQ=255)                                            ccAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGAt                                                                                            <  1:1514111/140‑1 (MQ=255)                                                tAATATTCAGGACCCGTTAAGCCGCGTGAATGGTGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa                                                                                        <  1:471081/140‑1 (MQ=255)                                                tAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa                                                                                        <  2:1112223/140‑1 (MQ=255)                                                  atatTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt                                                                                      <  1:723649/140‑1 (MQ=255)                                                  atatTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt                                                                                      <  1:1346424/140‑1 (MQ=255)                                                      tCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGAACCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAACATGTCACTGATCCCATTGAga                                                                                  >  2:527847/1‑139 (MQ=255)                                                         ggACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTcac                                                                               <  2:333855/140‑1 (MQ=255)                                                         ggACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTcac                                                                               <  1:1124362/140‑1 (MQ=255)                                                          gACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTcaca                                                                              >  2:1132013/1‑140 (MQ=255)                                                            cccGTTAAGCCGCGCGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGACACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGa                                                                            >  2:87889/1‑140 (MQ=255)                                                             ccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGaa                                                                           >  1:995828/1‑140 (MQ=255)                                                             ccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGaa                                                                           >  2:1063981/1‑140 (MQ=255)                                                             ccGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGaa                                                                           >  1:1074978/1‑140 (MQ=255)                                                               gTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcg                                                                         >  1:846672/1‑140 (MQ=255)                                                               gTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcg                                                                         >  1:78523/1‑140 (MQ=255)                                                                 tAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcgcg                                                                       <  1:819173/140‑1 (MQ=255)                                                                 tAAGCCGCGTGAATGGCGTAGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCGAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcgcg                                                                       <  2:1444823/140‑1 (MQ=255)                                                                    gCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAg                                                                    >  2:67153/1‑140 (MQ=255)                                                                      cgcgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGAt                                                                  <  2:1499295/140‑1 (MQ=255)                                                                       gcgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGAtt                                                                 >  2:546887/1‑140 (MQ=255)                                                                        cgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAAAACGCGCAGATTg                                                                >  1:408779/1‑140 (MQ=255)                                                                          tGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCg                                                              >  1:1246033/1‑140 (MQ=255)                                                                                 cGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGc                                                       >  2:68909/1‑140 (MQ=255)                                                                                     ggggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACt                                                   <  2:828672/140‑1 (MQ=255)                                                                                     ggggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACt                                                   <  1:574176/140‑1 (MQ=255)                                                                                     ggggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACt                                                   <  1:281818/140‑1 (MQ=255)                                                                                     ggggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACt                                                   <  2:1287709/140‑1 (MQ=255)                                                                                      gggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACtt                                                  >  2:674546/1‑140 (MQ=255)                                                                                       ggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTtg                                                 <  2:112895/140‑1 (MQ=255)                                                                                         atatCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTtggt                                               <  2:1247559/140‑1 (MQ=255)                                                                                            tCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTggtggt                                            <  1:87889/140‑1 (MQ=255)                                                                                              gATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTac                                          <  2:1009002/140‑1 (MQ=255)                                                                                                tGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGAGGTacac                                        >  2:770227/1‑140 (MQ=255)                                                                                                    tATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTc                                    <  2:1019575/140‑1 (MQ=255)                                                                                                    tATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTc                                    >  1:273764/1‑140 (MQ=255)                                                                                                      tGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTcca                                  >  1:84629/1‑139 (MQ=255)                                                                                                      tGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCg                                  <  2:846672/140‑1 (MQ=255)                                                                                                          tCGCAATATTCCATGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGa                              >  2:156639/1‑140 (MQ=255)                                                                                                                       tGCGTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTc                 >  2:837281/1‑140 (MQ=255)                                                                                                                          gTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAAc              >  1:917046/1‑140 (MQ=255)                                                                                                                          gTATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAAc              >  2:877090/1‑140 (MQ=255)                                                                                                                           tATCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACg             >  1:1371415/1‑140 (MQ=255)                                                                                                                             tCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGcc           >  1:519793/1‑140 (MQ=255)                                                                                                                             tCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGcc           >  1:1388001/1‑140 (MQ=255)                                                                                                                             tCTGGCTGGACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGcc           >  2:218535/1‑140 (MQ=255)                                                                                                                                    ggACCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCCACCATTa    <  1:68909/140‑1 (MQ=255)                                                                                                                                      aCCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCCACCATTAAc  >  2:735673/1‑140 (MQ=255)                                                                                                                                      aCCCAGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCCACCATTAAc  >  1:1028555/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ACCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCCACCATTAAC  >  NC_000913/2588027‑2588299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑