Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,933	T→C	Y136C (TAC→TGC)	trmD ←	tRNA m(1)G37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,933	0	T	C	100.0%	335.5 / NA	90	Y136C (TAC→TGC)	trmD	tRNA m(1)G37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/50);  total (40/50)

GTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTC  >  NC_000913/2744800‑2745068                                                                                                                                      |                                                                                                                                        gcgCGGGCAATCCACTAAACCTTCAGCAAAGGAATCTTCCGATGCCGAGGCTTCATTGCTCAGTACTTCTGGAATAAACCGGGAAACGAAGGCAATCAGCGACATTGCTGGCAACTCACCACCACCGAGAACGCAAgcgc                                                                                                                                   >  2:1399923/3‑140 (MQ=255) gTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGc                                                                                                                                   >  1:111089/1‑140 (MQ=255)    cGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGa                                                                                                                                >  1:24669/1‑140 (MQ=255)     gggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACTACCACTGAGAACGCAATCGCCGAt                                                                                                                               <  1:518889/140‑1 (MQ=255)     gggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGAt                                                                                                                               >  2:89074/1‑140 (MQ=255)     gggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGAt                                                                                                                               >  1:671455/1‑140 (MQ=255)      ggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGAtt                                                                                                                              >  2:1028593/1‑140 (MQ=255)      ggCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGAtt                                                                                                                              >  2:1336785/1‑140 (MQ=255)       gCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTg                                                                                                                             >  1:1129736/1‑140 (MQ=255)         aaTCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGAc                                                                                                                           <  1:1437613/140‑1 (MQ=255)          aTCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGAcc                                                                                                                          >  1:1526442/1‑140 (MQ=255)           tCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCa                                                                                                                         >  2:385061/1‑140 (MQ=255)                     cTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCa                                                                                                               >  2:355661/1‑140 (MQ=255)                     cTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCa                                                                                                               >  1:1255165/1‑140 (MQ=255)                     cTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCa                                                                                                               >  1:570687/1‑140 (MQ=255)                     cTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCa                                                                                                               >  1:1237775/1‑140 (MQ=255)                       tCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAAt                                                                                                             <  2:1327218/140‑1 (MQ=255)                       tCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAAt                                                                                                             <  1:409773/140‑1 (MQ=255)                        cAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAAtt                                                                                                            <  1:1439201/140‑1 (MQ=255)                        cAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAAtt                                                                                                            <  1:1196660/140‑1 (MQ=255)                         aGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAAttt                                                                                                           <  2:1245224/140‑1 (MQ=255)                              aGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGtt                                                                                                      >  2:525143/1‑140 (MQ=255)                               ggAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGttt                                                                                                     <  2:17761/140‑1 (MQ=255)                                gAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTg                                                                                                    >  2:868449/1‑140 (MQ=255)                                    cTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATc                                                                                                <  1:1399923/140‑1 (MQ=255)                                     ttCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCa                                                                                               >  1:1387702/1‑140 (MQ=255)                                       ccGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcg                                                                                             <  2:1219170/140‑1 (MQ=255)                                       ccGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcg                                                                                             <  1:711346/140‑1 (MQ=255)                                        cGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTC‑TCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  2:671455/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:896203/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:571684/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  2:509550/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  2:111089/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  2:1375889/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:284382/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:226297/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:216219/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  2:24669/140‑1 (MQ=255)                                         gTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCAcgcg                                                                                           <  1:1428328/140‑1 (MQ=255)                                          ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                                                                          <  2:1494210/140‑1 (MQ=255)                                          ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                                                                          <  1:1028593/140‑1 (MQ=255)                                          ttGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACgcgc                                                                                          <  1:332478/140‑1 (MQ=255)                                           tGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCt                                                                                         <  1:868449/140‑1 (MQ=255)                                             ccGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCa                                                                                       >  1:572595/1‑140 (MQ=255)                                             ccGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCa                                                                                       <  2:570687/140‑1 (MQ=255)                                             ccGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCa                                                                                       >  2:602417/1‑140 (MQ=255)                                              cGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCAt                                                                                      >  2:1075217/1‑140 (MQ=255)                                                 ggCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTGAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCta                                                                                   <  2:443344/140‑1 (MQ=255)                                                 ggCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCta                                                                                   <  1:1336785/140‑1 (MQ=255)                                                 ggCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCta                                                                                   >  1:302654/1‑140 (MQ=255)                                                 ggCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCta                                                                                   <  1:385061/140‑1 (MQ=255)                                                 ggCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCta                                                                                   <  1:965285/140‑1 (MQ=255)                                                       aTGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCtt                                                                             >  2:336561/1‑140 (MQ=255)                                                            ccAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAg                                                                        >  1:1373567/1‑140 (MQ=255)                                                            ccAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAg                                                                        <  2:1089352/140‑1 (MQ=255)                                                               gTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGa                                                                     <  1:743191/140‑1 (MQ=255)                                                               gTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGa                                                                     <  2:1245819/140‑1 (MQ=255)                                                                 aCTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGAcc                                                                   <  1:1173058/140‑1 (MQ=255)                                                                   tCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGc                                                                 >  2:124767/1‑140 (MQ=255)                                                                     ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac                                                               >  2:659901/1‑140 (MQ=255)                                                                     ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac                                                               >  1:852097/1‑140 (MQ=255)                                                                     ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac                                                               >  1:1139909/1‑140 (MQ=255)                                                                     ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGcac                                                               >  1:354299/1‑140 (MQ=255)                                                                       ggAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCacac                                                             <  1:367393/140‑1 (MQ=255)                                                                        gAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACAcc                                                            >  2:1104738/1‑140 (MQ=255)                                                                                          aGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTc                                          >  2:299837/1‑140 (MQ=255)                                                                                               aTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                     <  1:381911/140‑1 (MQ=255)                                                                                               aTCAGCGTCATTGCTGGGATCTCTCCTCCACTGAGAGCGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                     <  2:1129736/140‑1 (MQ=255)                                                                                                 cAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCa                                   <  1:525143/140‑1 (MQ=255)                                                                                                  aGCGTCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAg                                  >  1:181874/1‑140 (MQ=255)                                                                                                      tCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCg                              >  2:229735/1‑140 (MQ=255)                                                                                                      tCATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCg                              >  2:1234629/1‑140 (MQ=255)                                                                                                       cATTGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGc                             <  1:692993/140‑1 (MQ=255)                                                                                                         ttGCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTg                           <  1:89074/140‑1 (MQ=255)                                                                                                           gCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGAc                         <  1:2020/140‑1 (MQ=255)                                                                                                           gCTGGTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGAc                         <  2:572595/140‑1 (MQ=255)                                                                                                               gTAACTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGccc                     >  1:901316/1‑140 (MQ=255)                                                                                                                 aaCTCACCACCACTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGc                   <  2:861367/140‑1 (MQ=255)                                                                                                                      accaccacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGAt              <  2:1373567/140‑1 (MQ=255)                                                                                                                      accaccacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGAt              >  1:793833/1‑140 (MQ=255)                                                                                                                       ccaccacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATc             >  1:906594/1‑140 (MQ=255)                                                                                                                        caccacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCa            <  1:350020/140‑1 (MQ=255)                                                                                                                        caccacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCa            >  1:664781/1‑140 (MQ=255)                                                                                                                         accacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCGATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCaa           >  2:863150/1‑140 (MQ=255)                                                                                                                         accacTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCaa           >  2:582808/1‑140 (MQ=255)                                                                                                                             cTGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt       >  2:1306603/1‑140 (MQ=255)                                                                                                                              tGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg      <  2:1339327/140‑1 (MQ=255)                                                                                                                              tGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg      <  1:1104738/140‑1 (MQ=255)                                                                                                                              tGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg      >  1:1073618/1‑140 (MQ=255)                                                                                                                              tGAGAACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg      <  1:1000039/140‑1 (MQ=255)                                                                                                                                tgaACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCGTCGTAGCGTCCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCg    <  2:1237775/139‑1 (MQ=255)                                                                                                                                agaACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCg    <  1:162001/140‑1 (MQ=255)                                                                                                                                agaACGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCg    <  1:355661/140‑1 (MQ=255)                                                                                                                                  aaCGCAATCGCCGATTGACGAGTCTTCGTCAATTTCGGTTTGGATTACGCACTCATCTATTCCTTCCTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTc  <  2:1222004/140‑1 (MQ=255)                                                                                                                                  aaCGCAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTc  <  2:181874/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGTACTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTC  >  NC_000913/2744800‑2745068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑