Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,878	T→C	N45S (AAC→AGC)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,878	0	T	C	100.0%	275.2 / NA	73	N45S (AAC→AGC)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/45);  total (28/45)

GTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTC  >  NC_000913/2821746‑2822009                                                                                                                                     |                                                                                                                                    gTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATccc                                                                                                                              <  1:510212/140‑1 (MQ=255) gTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATccc                                                                                                                              >  2:794734/1‑140 (MQ=255)   aTGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCgg                                                                                                                            >  2:771387/1‑140 (MQ=255)   aTGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCgg                                                                                                                            <  1:1391753/140‑1 (MQ=255)     ggTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCg                                                                                                                          >  2:1185791/1‑140 (MQ=255)         aCGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTgg                                                                                                                      >  1:931614/1‑140 (MQ=255)           gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  2:1282670/1‑140 (MQ=255)           gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  1:983065/1‑140 (MQ=255)           gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCCTGTCGACCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  2:1173670/1‑140 (MQ=255)            cgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTaa                                                                                                                   >  1:1437349/1‑140 (MQ=255)                gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAaacaa                                                                                                               >  2:1424039/1‑140 (MQ=255)                      ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCCCGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGt                                                                                                         <  2:1227568/140‑1 (MQ=255)                          cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1086487/140‑1 (MQ=255)                          cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1374714/140‑1 (MQ=255)                          cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1381409/140‑1 (MQ=255)                           gTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGt                                                                                                    <  2:66154/140‑1 (MQ=255)                              ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGt                                                                                                 <  2:345956/140‑1 (MQ=255)                                ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTa                                                                                               <  1:896861/140‑1 (MQ=255)                                ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTCGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTa                                                                                               <  1:775254/140‑1 (MQ=255)                                  aGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATg                                                                                             <  2:129075/140‑1 (MQ=255)                                  aGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATg                                                                                             <  2:399197/140‑1 (MQ=255)                                      cGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGt                                                                                         >  1:148056/1‑140 (MQ=255)                                       gTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTa                                                                                        >  2:117150/1‑140 (MQ=255)                                         tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTAcc                                                                                      >  2:1230523/1‑140 (MQ=255)                                             tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAggg                                                                                  >  2:936499/1‑140 (MQ=255)                                             tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAggg                                                                                  <  1:718255/140‑1 (MQ=255)                                               tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAg                                                                                <  1:1176263/140‑1 (MQ=255)                                               tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAg                                                                                <  1:443227/140‑1 (MQ=255)                                                  caccCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAgctg                                                                             <  1:143686/140‑1 (MQ=255)                                                    cccGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAgctgct                                                                           <  2:93020/140‑1 (MQ=255)                                                     ccGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGctgctg                                                                          >  1:1408441/1‑140 (MQ=255)                                                         acgcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCaa                                                                      <  1:1282670/140‑1 (MQ=255)                                                         acgcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCaa                                                                      <  1:1173670/140‑1 (MQ=255)                                                           gcacgccGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  2:634565/140‑1 (MQ=255)                                                           gcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  1:216389/140‑1 (MQ=255)                                                           gcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  1:794734/140‑1 (MQ=255)                                                                caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:706444/140‑1 (MQ=255)                                                                caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:750691/140‑1 (MQ=255)                                                                caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:1185791/140‑1 (MQ=255)                                                                 aGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGa                                                              <  2:411394/140‑1 (MQ=255)                                                                   cGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATg                                                            >  1:1166335/1‑140 (MQ=255)                                                                       gggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTccc                                                        <  1:115387/140‑1 (MQ=255)                                                                        ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:1184920/140‑1 (MQ=255)                                                                        ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:676485/140‑1 (MQ=255)                                                                        ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:117150/140‑1 (MQ=255)                                                                        ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:637859/140‑1 (MQ=255)                                                                        ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  2:895654/140‑1 (MQ=255)                                                                         gAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCtt                                                      >  1:857465/1‑140 (MQ=255)                                                                             tGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTa                                                  >  2:628683/1‑140 (MQ=255)                                                                               gTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATg                                                >  2:968706/1‑140 (MQ=255)                                                                                   cgcgGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACAACCTGATGTCCCTTACTATGGaaa                                            <  2:1149452/140‑1 (MQ=255)                                                                                    gcgGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGaaaa                                           >  2:1078594/1‑140 (MQ=255)                                                                                       ggAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGt                                        >  2:588791/1‑140 (MQ=255)                                                                                       ggAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGt                                        >  1:544866/1‑140 (MQ=255)                                                                                            aatTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCgaga                                   <  1:710722/140‑1 (MQ=255)                                                                                              ttACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGaaa                                 <  2:51865/140‑1 (MQ=255)                                                                                               tACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGG‑ACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACg                               <  2:931614/140‑1 (MQ=255)                                                                                                  gCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGCACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGcc                             >  2:385236/1‑140 (MQ=255)                                                                                                      gTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGAc                         >  2:101462/1‑140 (MQ=255)                                                                                                      gTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGAc                         >  1:1220444/1‑140 (MQ=255)                                                                                                       tCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACg                        <  1:936499/140‑1 (MQ=255)                                                                                                        cGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACgg                       >  1:1388457/1‑140 (MQ=255)                                                                                                           gCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtc                    >  1:682670/1‑140 (MQ=255)                                                                                                            cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  2:983065/140‑1 (MQ=255)                                                                                                            cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  1:15450/140‑1 (MQ=255)                                                                                                            cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  2:148056/140‑1 (MQ=255)                                                                                                             ccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtctc                  <  1:667133/140‑1 (MQ=255)                                                                                                                aGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGc               <  2:1437349/140‑1 (MQ=255)                                                                                                                      acaTCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGct         >  1:829396/1‑140 (MQ=255)                                                                                                                       caTCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGctc        <  1:1424039/140‑1 (MQ=255)                                                                                                                           cTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGCACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGc    >  2:141064/1‑140 (MQ=255)                                                                                                                            ttGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCt   <  1:588791/140‑1 (MQ=255)                                                                                                                            ttGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCt   <  2:857465/140‑1 (MQ=255)                                                                                                                             tGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTc  >  1:1517301/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    GTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTC  >  NC_000913/2821746‑2822009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑