Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,059,205	T→C	T41A (ACA→GCA)	rpiA ←	ribose‑5‑phosphate isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,059,205	0	T	C	100.0%	289.2 / NA	79	T41A (ACA→GCA)	rpiA	ribose‑5‑phosphate isomerase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/42);  total (37/42)

TTCATCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGT  >  NC_000913/3059071‑3059340                                                                                                                                       |                                                                                                                                        ttCATCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGa                                                                                                                                    >  1:617867/1‑140 (MQ=255)  tCATCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAg                                                                                                                                   <  1:1541402/140‑1 (MQ=255)   cATCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAAGTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAgc                                                                                                                                  >  2:856749/1‑140 (MQ=255)    aTCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAgcg                                                                                                                                 <  1:757600/140‑1 (MQ=255)    aTCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAgcg                                                                                                                                 <  2:640033/140‑1 (MQ=255)      cTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGTAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGcgcg                                                                                                                               >  2:302476/1‑140 (MQ=255)      cTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGcgcg                                                                                                                               <  2:107665/140‑1 (MQ=255)         cgcCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGTGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCa                                                                                                                            >  2:243217/1‑140 (MQ=255)           ccATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAAt                                                                                                                          <  2:1401021/140‑1 (MQ=255)                        tGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGGACCGAGCGCGTCAATAAAGTGTgcggcg                                                                                                             >  2:216895/1‑140 (MQ=255)                        tGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGACGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGCCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTgcggcg                                                                                                             >  1:399547/1‑140 (MQ=255)                         gCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGcggcgg                                                                                                            >  1:856496/1‑140 (MQ=255)                         gCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGcggcgg                                                                                                            >  2:757156/1‑140 (MQ=255)                         gCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGcggcgg                                                                                                            >  1:171283/1‑140 (MQ=255)                          ccAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAACCGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGt                                                                                                           <  2:1111313/140‑1 (MQ=255)                          ccAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGcggcggg                                                                                                           >  1:544595/1‑139 (MQ=255)                          ccAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGt                                                                                                           <  2:117265/140‑1 (MQ=255)                           cAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCGTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTg                                                                                                          <  1:188999/140‑1 (MQ=255)                            aaGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTgg                                                                                                         <  1:3066/140‑1 (MQ=255)                            aaGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTgg                                                                                                         >  2:1240434/1‑140 (MQ=255)                            aaGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTgg                                                                                                         <  1:906921/140‑1 (MQ=255)                            aaGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTgg                                                                                                         >  2:994773/1‑140 (MQ=255)                             aGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGa                                                                                                        >  1:1326170/1‑140 (MQ=255)                               gCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAAc                                                                                                      >  2:473246/1‑140 (MQ=255)                                cTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAAcc                                                                                                     <  1:572539/140‑1 (MQ=255)                                  gTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCGCCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTg                                                                                                   >  2:399911/1‑140 (MQ=255)                                   tCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGt                                                                                                  >  1:394274/1‑140 (MQ=255)                                    cGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTa                                                                                                 <  2:255696/140‑1 (MQ=255)                                    cGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTa                                                                                                 <  1:1458951/140‑1 (MQ=255)                                      atttCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTAcc                                                                                               <  2:960454/138‑1 (MQ=255)                                      aCTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTAcc                                                                                               <  2:1017744/140‑1 (MQ=255)                                      aCTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTAcc                                                                                               <  1:400368/140‑1 (MQ=255)                                        ttCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTa                                                                                             >  2:1459812/1‑140 (MQ=255)                                         tCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTac                                                                                            >  1:224735/1‑140 (MQ=255)                                         tCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTac                                                                                            >  1:1468105/1‑140 (MQ=255)                                            ttGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACAcc                                                                                         <  2:136759/140‑1 (MQ=255)                                             tGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACca                                                                                        >  2:521251/1‑140 (MQ=255)                                              gagaTCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACcaa                                                                                       <  1:1352535/140‑1 (MQ=255)                                                 aTCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACcaacaa                                                                                    >  2:983466/1‑140 (MQ=255)                                                 aTCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACcaacaa                                                                                    >  1:1342502/1‑140 (MQ=255)                                                      aaaCGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTg                                                                               <  2:1033704/140‑1 (MQ=255)                                                         cGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTTGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCg                                                                            >  1:1495383/1‑140 (MQ=255)                                                         cGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCg                                                                            >  1:1232267/1‑140 (MQ=255)                                                           tGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCggg                                                                          <  2:1241619/140‑1 (MQ=255)                                                           tGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCggg                                                                          >  1:595162/1‑140 (MQ=255)                                                            gAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGc                                                                         <  1:216895/140‑1 (MQ=255)                                                             aaTGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCt                                                                        <  2:479274/140‑1 (MQ=255)                                                                    aGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATa                                                                 >  2:517212/1‑140 (MQ=255)                                                                       cTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTg                                                              <  2:171283/140‑1 (MQ=255)                                                                       cTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTg                                                              <  1:1247395/140‑1 (MQ=255)                                                                         tttcagtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGaa                                                            >  2:431612/1‑140 (MQ=255)                                                                          ttcagtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAg                                                           >  2:959929/1‑140 (MQ=255)                                                                            cagtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTg                                                         <  2:856496/140‑1 (MQ=255)                                                                              gtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTgcc                                                       <  1:473246/140‑1 (MQ=255)                                                                              gtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTgcc                                                       <  2:1342502/140‑1 (MQ=255)                                                                              gtttttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTgcc                                                       <  1:617192/140‑1 (MQ=255)                                                                                 tttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTgccgcc                                                    <  1:1459812/140‑1 (MQ=255)                                                                                 tttcagtGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTgccgcc                                                    >  1:71413/1‑140 (MQ=255)                                                                                         gAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTAc                                            >  1:1124924/1‑140 (MQ=255)                                                                                             cATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCt                                        >  1:1522557/1‑140 (MQ=255)                                                                                             cATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCt                                        <  1:511876/140‑1 (MQ=255)                                                                                               tCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCttt                                      >  1:1003640/1‑140 (MQ=255)                                                                                                cTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCtgtt                                     >  1:783829/1‑140 (MQ=255)                                                                                                 tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCttttt                                    <  2:617867/140‑1 (MQ=255)                                                                                                 tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCttttt                                    <  1:757156/140‑1 (MQ=255)                                                                                                 tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCttttt                                    <  2:399547/140‑1 (MQ=255)                                                                                                      tGGAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAAt                               <  2:472683/140‑1 (MQ=255)                                                                                                        gAAACGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTc                             >  2:837307/1‑140 (MQ=255)                                                                                                           aCGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATc                          <  1:983466/140‑1 (MQ=255)                                                                                                            cGGCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATcc                         <  2:1232267/140‑1 (MQ=255)                                                                                                             ggCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCt                        <  1:856749/140‑1 (MQ=255)                                                                                                             ggCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCt                        >  1:1137422/1‑140 (MQ=255)                                                                                                              gCCCCTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTg                       <  1:517212/140‑1 (MQ=255)                                                                                                               ccccTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGc                      >  1:642670/1‑140 (MQ=255)                                                                                                                 ccTTCAATCTGGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGt                    <  2:713851/140‑1 (MQ=255)                                                                                                                     cAATCTTGCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                <  2:1495383/140‑1 (MQ=255)                                                                                                                            gCCTTTCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGttt         >  2:313777/1‑140 (MQ=255)                                                                                                                               tttCATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc      >  1:960302/1‑140 (MQ=255)                                                                                                                                  cATTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg   <  2:1326170/140‑1 (MQ=255)                                                                                                                                   aTTGCACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtgt  <  2:1124924/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCATCTGCGCCATCAACGTAGATGCCAAGGCTGTCGACTTCGTTGAGATCAAAAACGTGAATGCCGAGGCTTTTCAGTTTTTCAGTGGAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGT  >  NC_000913/3059071‑3059340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑