Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,153,184	A→G	K317E (AAA→GAA)	metC →	cystathionine beta‑lyase/L‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,153,184	0	A	G	100.0%	322.7 / NA	86	K317E (AAA→GAA)	metC	cystathionine beta‑lyase/L‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/53);  total (33/53)

TGAAAGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGCCCA  >  NC_000913/3153047‑3153315                                                                                                                                          |                                                                                                                                    tGAAAGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGgaa                                                                                                                                   <  2:794094/140‑1 (MQ=255)   aaaGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGgaaaa                                                                                                                                 <  1:359485/140‑1 (MQ=255)    aaGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGgaaaaa                                                                                                                                <  1:555265/140‑1 (MQ=255)    aaGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGgaaaaa                                                                                                                                <  2:648460/140‑1 (MQ=255)      gTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACt                                                                                                                              <  1:525764/140‑1 (MQ=255)      gTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACt                                                                                                                              <  2:385724/140‑1 (MQ=255)        ggCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCa                                                                                                                            <  1:1395282/140‑1 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGTGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  1:737465/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  2:18362/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  2:338105/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  1:1122748/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  1:867129/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  1:629108/1‑140 (MQ=255)         gCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCaa                                                                                                                           >  2:972416/1‑140 (MQ=255)                 gctggcAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAag                                                                                                                   <  1:1060829/140‑1 (MQ=255)                   tggcAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAagag                                                                                                                 >  1:767073/1‑140 (MQ=255)                   gggcAGAACGTCCGCAAGTTGCTCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTGCCTTTGTGCTTAAGGAAAAACTCAATAATGAagag                                                                                                                 <  1:1223635/139‑1 (MQ=255)                     gcAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCt                                                                                                               <  1:131247/140‑1 (MQ=255)                     gcAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCt                                                                                                               <  1:575657/140‑1 (MQ=255)                        gAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGc                                                                                                            <  1:926380/140‑1 (MQ=255)                         aaCATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCg                                                                                                           <  2:1341079/140‑1 (MQ=255)                          aCATCCGCAAGTTGTGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGa                                                                                                          <  2:737465/140‑1 (MQ=255)                          aCATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGa                                                                                                          <  1:626994/140‑1 (MQ=255)                          aCATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGa                                                                                                          <  1:994490/140‑1 (MQ=255)                          aCATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGa                                                                                                          <  1:225871/140‑1 (MQ=255)                          aCATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGa                                                                                                          <  2:778389/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         >  2:1073640/1‑140 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         <  2:1197173/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         <  1:182411/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         <  1:1436440/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         <  1:518306/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         <  1:713748/140‑1 (MQ=255)                           cATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGCCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGaa                                                                                                         >  2:815680/1‑140 (MQ=255)                             tCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACt                                                                                                       <  2:106191/140‑1 (MQ=255)                             tCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACt                                                                                                       <  2:659192/140‑1 (MQ=255)                               cGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTAt                                                                                                     >  1:311164/1‑140 (MQ=255)                               cGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTAt                                                                                                     >  2:378218/1‑140 (MQ=255)                                  aaGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTg                                                                                                  <  2:1246282/140‑1 (MQ=255)                                   aGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTgg                                                                                                 >  1:1051203/1‑140 (MQ=255)                                    gTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGa                                                                                                >  1:375277/1‑140 (MQ=255)                                     ttGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGAt                                                                                               <  1:381634/140‑1 (MQ=255)                                        cgcgAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAAc                                                                                            >  1:336993/1‑140 (MQ=255)                                         gcgAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACt                                                                                           <  2:101382/140‑1 (MQ=255)                                           gAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTc                                                                                         <  1:912965/140‑1 (MQ=255)                                            aGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCa                                                                                        <  1:1195584/140‑1 (MQ=255)                                            aGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCa                                                                                        <  2:1469534/140‑1 (MQ=255)                                              ttAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGt                                                                                      <  1:1078643/140‑1 (MQ=255)                                              ttAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGt                                                                                      <  2:284251/140‑1 (MQ=255)                                              ttAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGt                                                                                      <  1:1472918/140‑1 (MQ=255)                                                 accaccCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTa                                                                                   <  1:1003593/140‑1 (MQ=255)                                                  ccaccCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTAt                                                                                  <  2:1468247/140‑1 (MQ=255)                                                      ccTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAg                                                                              <  1:1073640/140‑1 (MQ=255)                                                      ccTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAg                                                                              <  2:767073/140‑1 (MQ=255)                                                      ccTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAg                                                                              <  1:344315/140‑1 (MQ=255)                                                       cTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGc                                                                             >  1:1376638/1‑140 (MQ=255)                                                       cTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGc                                                                             >  1:12045/1‑140 (MQ=255)                                                       cTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGc                                                                             <  1:1061264/140‑1 (MQ=255)                                                        tGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCa                                                                            <  1:338105/140‑1 (MQ=255)                                                          ctctGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATg                                                                          <  1:1503237/140‑1 (MQ=255)                                                               ccTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTa                                                                     >  1:59391/1‑140 (MQ=255)                                                                cTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTAc                                                                    >  2:1097395/1‑140 (MQ=255)                                                                    cAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGt                                                                <  1:18362/140‑1 (MQ=255)                                                                        aaaGGTCACGAATTCTGGAAACGAGACTTTACGGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTgggg                                                            <  2:132805/140‑1 (MQ=255)                                                                        aaaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTTAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTgggg                                                            <  2:222741/140‑1 (MQ=255)                                                                        aaaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTgggg                                                            <  1:937591/140‑1 (MQ=255)                                                                        aaaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTgggg                                                            <  1:922317/140‑1 (MQ=255)                                                                         aaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGc                                                           <  2:442937/140‑1 (MQ=255)                                                                         aaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGc                                                           <  1:65317/140‑1 (MQ=255)                                                                         aaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGc                                                           >  1:1384972/1‑140 (MQ=255)                                                                         aaGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGc                                                           >  2:707003/1‑140 (MQ=255)                                                                            gTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCggg                                                        >  1:621005/1‑140 (MQ=255)                                                                               aCGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTAt                                                     >  2:189372/1‑140 (MQ=255)                                                                                 gAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGa                                                   >  2:1225850/1‑140 (MQ=255)                                                                                  aaTTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGaa                                                  <  2:1051203/140‑1 (MQ=255)                                                                                     tCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCg                                               <  1:388833/140‑1 (MQ=255)                                                                                     tCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCg                                               <  1:582594/140‑1 (MQ=255)                                                                                              gagaCTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTg                                      <  1:1097395/140‑1 (MQ=255)                                                                                                  cTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCaa                                  <  2:375277/140‑1 (MQ=255)                                                                                                   tttACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCaaa                                 <  1:218545/140‑1 (MQ=255)                                                                                                        aGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAAc                            <  1:891282/140‑1 (MQ=255)                                                                                                        aGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAAc                            <  1:909670/140‑1 (MQ=255)                                                                                                             gcagcGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGTTTCTGGCAAATCAACCAGaa                       >  2:1545991/1‑140 (MQ=255)                                                                                                             gcagcGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGaa                       >  2:712513/1‑140 (MQ=255)                                                                                                              cagcGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAAc                      >  2:1505841/1‑140 (MQ=255)                                                                                                               agcGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACa                     >  1:843592/1‑140 (MQ=255)                                                                                                                 cGGGCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACata                   >  1:124415/1‑140 (MQ=255)                                                                                                                    gCTATTTTCCTTTGTGCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATcg                >  1:773649/1‑140 (MQ=255)                                                                                                                                tgtgCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGcc    >  1:826392/1‑140 (MQ=255)                                                                                                                                tgtgCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGcc    >  2:888290/1‑140 (MQ=255)                                                                                                                                  tgCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGATCTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGGGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGCCca  <  1:211984/140‑1 (MQ=255)                                                                                                                                  tgCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGCCca  >  2:562688/1‑140 (MQ=255)                                                                                                                                  tgCTTAAGGAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGCCca  >  2:1284514/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    TGAAAGTGGCTGAATGGCTGGCAGAACATCCGCAAGTTGCGCGAGTTAACCACCCTGCTCTGCCTGGCAGTAAAGGTCACGAATTCTGGAAACGAGACTTTACAGGCAGCAGCGGGCTATTTTCCTTTGTGCTTAAGAAAAAACTCAATAATGAAGAGCTGGCGAACTATCTGGATAACTTCAGTTTATTCAGCATGGCCTACTCGTGGGGCGGGTATGAATCGTTGATCCTGGCAAATCAACCAGAACATATCGCCGCCATTCGCCCA  >  NC_000913/3153047‑3153315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑