Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,945	T→C	A334A (GCT→GCC)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,945	0	T	C	100.0%	194.6 / NA	54	A334A (GCT→GCC)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/37);  total (17/37)

CGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTG  >  NC_000913/3240810‑3241080                                                                                                                                        |                                                                                                                                        cGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCtctc                                                                                                                                     <  1:1223357/140‑1 (MQ=255)     tCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCCGCAACCACTATTACCCCGTTTGCGCTGGCTGCGGGTGAAAAGCCGGGGGTTCCGGGCCGGCTGCCCCCCGCGCCGCGGTTGGGCGGGGGGGGCTCTCtgtg                                                                                                                                 >  2:1101462/1‑140 (MQ=255)                       accaTCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTATGTGTGCCTGTGGCGCATCCgg                                                                                                               <  1:620542/140‑1 (MQ=255)                         caTCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCg                                                                                                             <  1:1405452/140‑1 (MQ=255)                             aaTATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGGGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                         <  2:1092659/140‑1 (MQ=255)                                atGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  1:722799/140‑1 (MQ=255)                                atGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGCGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  1:1174559/140‑1 (MQ=255)                                atGGCGGGCGAAGCAATCACTCTTACCGGGTTGACGCTGGTTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCGCGGCGCTGCTGTTGAGCGTGGTGGCCTATCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  2:1027491/140‑1 (MQ=255)                                 tGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggg                                                                                                     <  2:315558/140‑1 (MQ=255)                                   gcgggcgCAGCAATCACTATTGCCGTGTTGACGCTGGCTGCGGTTAATCCGCGGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggggg                                                                                                   <  2:164403/140‑1 (MQ=255)                                   gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggggg                                                                                                   <  1:1027598/140‑1 (MQ=255)                                     gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                 <  1:1044037/140‑1 (MQ=255)                                     gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                 <  1:157463/140‑1 (MQ=255)                                        cgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtctc                                                                                              <  1:824728/140‑1 (MQ=255)                                         gcagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTctct                                                                                             <  1:369028/140‑1 (MQ=255)                                          cagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctg                                                                                            <  1:397491/140‑1 (MQ=255)                                                      ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:1423763/140‑1 (MQ=255)                                                      ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:1439079/140‑1 (MQ=255)                                                      ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:792245/140‑1 (MQ=255)                                                         ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                             <  1:176204/140‑1 (MQ=255)                                                          cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                            <  1:1101462/140‑1 (MQ=255)                                                           gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                           <  1:753461/140‑1 (MQ=255)                                                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGTCTGCTGCTGGTCCCACCGGcc                                                                          >  2:1431558/1‑140 (MQ=255)                                                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCCGCTGCCGATCCCACTGGcc                                                                          >  2:421177/1‑140 (MQ=255)                                                             gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCt                                                                         >  1:347230/1‑140 (MQ=255)                                                              ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTg                                                                        >  1:365654/1‑140 (MQ=255)                                                              ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTg                                                                        >  1:274124/1‑140 (MQ=255)                                                              ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTCCCTCTTATCCCCcctgcctg                                                                        >  2:1055480/1‑140 (MQ=255)                                                                gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                      >  1:856658/1‑140 (MQ=255)                                                                        ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                              <  1:1378825/140‑1 (MQ=255)                                                                         tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                             <  1:1238072/140‑1 (MQ=255)                                                                           cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGt                                                           <  1:1306783/140‑1 (MQ=255)                                                                             gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGAt                                                         >  1:1041384/1‑140 (MQ=255)                                                                                 atccGCTGTGTATTCCGGTCGTTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCATCTCTGTGTGCCTGGGGCGCATCCGGGGTGGCGGGGGGGTCTCTGCTGCTGATCCCGCTGGCCTGTAATATGTTCGGTATTTCg                                                     <  2:735897/137‑1 (MQ=255)                                                                                 aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGAGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTCTTGTTCCCACTTTGCTGTAATATTGTCgggtgtttg                                                     >  2:277307/1‑133 (MQ=255)                                                                                   aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGTCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                   <  1:1091435/140‑1 (MQ=255)                                                                                   aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                   <  1:220689/140‑1 (MQ=255)                                                                                        gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGACCCTCCCCCTTAACCCAACGGCCTAGAACATGTTCGGGATTTCGAACGcta                                              >  2:146979/1‑140 (MQ=255)                                                                                           tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                           <  2:1546144/140‑1 (MQ=255)                                                                                            aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                          <  1:90197/140‑1 (MQ=255)                                                                                              tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGGATTTCGAACGATATCGccc                                        >  2:1267874/1‑139 (MQ=255)                                                                                               ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGGATTTTGAACGATATCGCCAt                                       >  2:1196864/1‑140 (MQ=255)                                                                                                   tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                   >  1:1236445/1‑140 (MQ=255)                                                                                                   tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCCGATCCCCCCGGCCCGTAATAAGTTCGGGTTTTCGGACGAGATCCCCCTGCAg                                   >  2:457764/1‑140 (MQ=255)                                                                                                    cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg                                  <  1:919552/140‑1 (MQ=255)                                                                                                        cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                              <  1:1431558/140‑1 (MQ=255)                                                                                                                   gctgctGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt                   <  1:688607/140‑1 (MQ=255)                                                                                                                       ctgTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTat               <  1:421177/140‑1 (MQ=255)                                                                                                                          ttGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat            <  2:274124/140‑1 (MQ=255)                                                                                                                          ttGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat            <  2:365654/140‑1 (MQ=255)                                                                                                                               cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  1:1196864/140‑1 (MQ=255)                                                                                                                               cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  2:1236445/140‑1 (MQ=255)                                                                                                                               cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  2:856658/140‑1 (MQ=255)                                                                                                                                 tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTa     <  2:970339/140‑1 (MQ=255)                                                                                                                                 tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGGCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGTGGGTGCCGGCGGCTTTATCATCGGCgga     >  2:298597/1‑138 (MQ=255)                                                                                                                                 tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCCGCTGCTTATCCCACTGGCCTGTTAAATGTTTGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGCCCTCGGCTTTCTTATCCGCGTa     >  2:587984/1‑140 (MQ=255)                                                                                                                                    tggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTTTTCCTGCACATACCTCATGCCAGTAATAAGATAAGCATTTTGAACAAAATTGCCAACCAAGAGGGTGCCGTCGGCTTTATACTCGGCGtcctc  >  2:982708/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                                        CGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTG  >  NC_000913/3240810‑3241080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑