Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	278,775	G→A	R10C (CGC→TGC)	afuC ←	CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	278,775	0	G	A	100.0%	322.7 / NA	94	R10C (CGC→TGC)	afuC	CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/53);  total (41/53)

GCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATG  >  NC_000913/278645‑278910                                                                                                                                   |                                                                                                                                        gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAAc                                                                                                                                >  2:36875/1‑140 (MQ=255)  cAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACg                                                                                                                               >  2:707123/1‑140 (MQ=255)    aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGaa                                                                                                                             <  1:498262/140‑1 (MQ=255)        ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAAttt                                                                                                                         <  1:269575/140‑1 (MQ=255)          cgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAAttttt                                                                                                                       >  2:1311605/1‑140 (MQ=255)          cgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAAttttt                                                                                                                       <  2:1011568/140‑1 (MQ=255)          cgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAAttttt                                                                                                                       >  1:706127/1‑140 (MQ=255)          cgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAAttttt                                                                                                                       <  2:517474/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     >  1:1072371/1‑140 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  2:1451544/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  2:1195226/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     >  1:1351886/1‑140 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  1:744871/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  1:696278/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  1:1117626/140‑1 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     >  1:630106/1‑140 (MQ=255)            cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGCATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  2:1298741/140‑1 (MQ=255)            aagaagaGTGGTTTTGCCGCGGCCGGACGGGCCGAGCAGCGTCACGATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCt                                                                                                                     <  2:796013/134‑1 (MQ=255)             aaaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTg                                                                                                                    <  1:157301/140‑1 (MQ=255)                    tGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATa                                                                                                             >  2:1255307/1‑140 (MQ=255)                    tGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATa                                                                                                             >  2:1250675/1‑140 (MQ=255)                    tGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATa                                                                                                             >  2:1239252/1‑140 (MQ=255)                    tGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATa                                                                                                             <  1:34831/140‑1 (MQ=255)                    tGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATa                                                                                                             >  2:275700/1‑140 (MQ=255)                      gTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGt                                                                                                           >  2:339032/1‑140 (MQ=255)                      gTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGt                                                                                                           <  2:650261/140‑1 (MQ=255)                       ttttGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTg                                                                                                          <  1:64000/140‑1 (MQ=255)                         ttGCCGCAGCCGGACGGGCCGAGCAGCGTCGCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCACCGAAATTTTTCTGAGTCATAGTgcg                                                                                                        <  2:838533/140‑1 (MQ=255)                         ttGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTgcg                                                                                                        <  2:942207/140‑1 (MQ=255)                         ttGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTgcg                                                                                                        <  2:769787/140‑1 (MQ=255)                         ttGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTgcg                                                                                                        >  1:969507/1‑140 (MQ=255)                          tGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTgcgc                                                                                                       <  1:224292/140‑1 (MQ=255)                                 gCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTa                                                                                                >  2:263348/1‑140 (MQ=255)                                    ggACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGc                                                                                             >  1:458663/1‑140 (MQ=255)                                    ggACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGc                                                                                             >  1:472325/1‑140 (MQ=255)                                    ggACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGc                                                                                             <  2:148839/140‑1 (MQ=255)                                            cGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGtttt                                                                                     <  1:1535356/140‑1 (MQ=255)                                                cagcGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGc                                                                                 <  1:556699/140‑1 (MQ=255)                                                    gTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCtttt                                                                             >  2:973216/1‑140 (MQ=255)                                                    gTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCtttt                                                                             >  1:254582/1‑140 (MQ=255)                                                       aCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGaa                                                                          <  1:1403035/140‑1 (MQ=255)                                                       aCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGaa                                                                          <  2:1072371/140‑1 (MQ=255)                                                       aCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGaa                                                                          <  2:20372/140‑1 (MQ=255)                                                       aCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGaa                                                                          <  1:528233/140‑1 (MQ=255)                                                       aCCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGaa                                                                          <  1:1493331/140‑1 (MQ=255)                                                           tttGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACggg                                                                      <  2:1193738/140‑1 (MQ=255)                                                           tttGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACggg                                                                      <  2:458663/140‑1 (MQ=255)                                                           tttGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACggg                                                                      <  2:472325/140‑1 (MQ=255)                                                               ccccTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGt                                                                  >  2:603220/1‑140 (MQ=255)                                                               ccccTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGt                                                                  <  1:1500332/140‑1 (MQ=255)                                                                   tGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGATCGGGAGGTACGt                                                              >  2:911783/1‑140 (MQ=255)                                                                   tGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGt                                                              <  2:497991/140‑1 (MQ=255)                                                                    gCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTg                                                             <  1:1210410/140‑1 (MQ=255)                                                                     cGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAAc                                                                         >  1:771446/1‑127 (MQ=255)                                                                     cGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAAc                                                                         <  2:771446/127‑1 (MQ=255)                                                                         aTGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCa                                                        >  1:547610/1‑140 (MQ=255)                                                                            gTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCg                                                     >  1:160015/1‑140 (MQ=255)                                                                              gAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAt                                                   <  2:1351886/140‑1 (MQ=255)                                                                              gAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAt                                                   <  2:44056/140‑1 (MQ=255)                                                                              gAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAt                                                   <  2:1085264/140‑1 (MQ=255)                                                                              gAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAt                                                   <  1:845008/140‑1 (MQ=255)                                                                              gAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAt                                                   <  1:1239252/140‑1 (MQ=255)                                                                               aGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATc                                                  >  2:646894/1‑140 (MQ=255)                                                                                 gTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAg                                                >  1:402875/1‑140 (MQ=255)                                                                                  ttGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGc                                               >  2:824998/1‑140 (MQ=255)                                                                                   tGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGcc                                              <  2:254582/140‑1 (MQ=255)                                                                                    gATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCa                                             >  1:783943/1‑140 (MQ=255)                                                                                       atTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTc                                          <  1:36875/140‑1 (MQ=255)                                                                                        ttGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCa                                         <  1:1479810/140‑1 (MQ=255)                                                                                         tGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGGGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGTTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCaa                                        >  2:1462826/1‑140 (MQ=255)                                                                                           tCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAg                                      >  1:1273486/1‑140 (MQ=255)                                                                                              aTTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATg                                   >  2:1333589/1‑140 (MQ=255)                                                                                               ttACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGa                                  >  1:1011604/1‑140 (MQ=255)                                                                                               ttACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGa                                  >  1:535007/1‑140 (MQ=255)                                                                                                tACCGTATTACTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGaa                                 >  1:768092/1‑140 (MQ=255)                                                                                                         aCTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGc                        >  1:1079756/1‑140 (MQ=255)                                                                                                         aCTGCCAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGc                        <  2:706127/140‑1 (MQ=255)                                                                                                             ccAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGc                    >  1:304803/1‑140 (MQ=255)                                                                                                              cAAATCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCa                   <  2:969507/140‑1 (MQ=255)                                                                                                               aaaTCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCAt                  >  1:1408561/1‑140 (MQ=255)                                                                                                                aaTCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATc                 >  2:21765/1‑140 (MQ=255)                                                                                                                  tCGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATcac               <  1:1255307/140‑1 (MQ=255)                                                                                                                   cGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATcacc              <  1:1311605/140‑1 (MQ=255)                                                                                                                   cGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATcacc              <  2:522491/140‑1 (MQ=255)                                                                                                                   cGTTTAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATcacc              <  2:444151/140‑1 (MQ=255)                                                                                                                       tAGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGa          >  1:7761/1‑140 (MQ=255)                                                                                                                        aGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGAt         <  2:1182933/140‑1 (MQ=255)                                                                                                                        aGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGAt         <  1:339032/140‑1 (MQ=255)                                                                                                                        aGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGAt         <  1:646894/140‑1 (MQ=255)                                                                                                                        aGTGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTACATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGAt         <  1:588493/140‑1 (MQ=255)                                                                                                                          tGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCa                   <  2:1122910/128‑1 (MQ=255)                                                                                                                          tGACGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCa                   >  1:1122910/1‑128 (MQ=255)                                                                                                                            aCGTTGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGa     >  1:703129/1‑140 (MQ=255)                                                                                                                               ttGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATg  >  1:1244014/1‑140 (MQ=255)                                                                                                                               ttGCACAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATg  >  1:1284177/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        GCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATG  >  NC_000913/278645‑278910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑