Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,556	C→T	Q230* (CAG→TAG)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,556	0	C	T	100.0%	200.9 / NA	64	Q230* (CAG→TAG)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/27);  total (37/27)

GGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTG  >  NC_000913/3375423‑3375690                                                                                                                                      |                                                                                                                                       gggAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCag                                                                                                                                  >  2:1145155/1‑140 (MQ=255) gggAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCag                                                                                                                                  >  1:18488/1‑140 (MQ=255)  ggAATAAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCaga                                                                                                                                 >  1:812777/1‑140 (MQ=255)      tGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCaa                                                                                                                             >  1:892820/1‑140 (MQ=255)       gAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCaaa                                                                                                                            <  1:951651/140‑1 (MQ=255)          aaTTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAg                                                                                                                         <  2:590603/140‑1 (MQ=255)            ttGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGct                                                                                                                       <  1:663334/140‑1 (MQ=255)            ttGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGct                                                                                                                       <  1:1130061/140‑1 (MQ=255)            ttGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGct                                                                                                                       <  1:198025/140‑1 (MQ=255)             tGCCACGTACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGctc                                                                                                                      >  1:1511600/1‑140 (MQ=255)              gCCTCATTCTTCTGCCTCTCCCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGTCGATCTCCCACTTTTTCGTGCAACGCGCGTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGctct                                                                                                                     <  2:1367157/140‑1 (MQ=255)                cacaTACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCg                                                                                                                   >  2:69843/1‑140 (MQ=255)                  caTACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGAt                                                                                                                 <  1:452566/140‑1 (MQ=255)                         ttcccccTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCCCTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTcc                                                                                                          <  1:1512959/134‑1 (MQ=255)                          ttcCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTccc                                                                                                         >  1:417532/1‑140 (MQ=255)                           ccccctcccAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCCCCCACTTTTTCGTGCACCGCGCTTTTGACAAAAAAGATAAAAATATCAATCACAAATAGGCAGAGAAAAAAGCTCTCGATAATGTCccg                                                                                                        <  2:1271267/133‑1 (MQ=255)                           cccACTACAAGTTTCCGGTCCCCTCTTGCGTCATTATCTCGGTGGCAATCTCCCCCTTTTTCGTGCAACGCGTTTTTGACAAAAAAAATAAAAATATCAATCACGAATAGGCAGAGAAAAAAGCTCTCGATAATGCCccg                                                                                                        <  2:296874/139‑1 (MQ=255)                            ccACTCCCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCCCCCCCTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCccgc                                                                                                       <  1:709499/140‑1 (MQ=255)                            ccACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCccgc                                                                                                       >  1:1539778/1‑140 (MQ=255)                             cACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCccgcc                                                                                                      >  2:1199270/1‑140 (MQ=255)                             cACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCccgcc                                                                                                      >  2:1084848/1‑140 (MQ=255)                              cctcccAGCTTCCGGCCGCCTCTTGCGTCATTATCCCGGTGGCGATCCCCCACTTTTTCGTGCACCGCGCTTTTGACAAAAAAGATAAAAATATCAACCACGAATGGGCAGAGCAAAAAGCTCTCGATAATGTCccgccg                                                                                                     <  2:1260854/136‑1 (MQ=255)                              aCTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCccgccg                                                                                                     >  2:744106/1‑140 (MQ=255)                               ccaccaccTTCCGGCCCCCTCTTGCGTCATTATCTCGGTGGCGATCCCCCCCTTTTCCGGGCACCGCGCTTTTGACAAAAAAGATAAAAATATCAACCCCGAATAGGAAGAGCAAAAAGCTCTCGATAATGCCCcgccgc                                                                                                    <  2:207100/138‑1 (MQ=255)                                 aCCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctc                                                                                                  >  2:154034/1‑140 (MQ=255)                                 aCCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctc                                                                                                  >  1:679598/1‑140 (MQ=255)                                 aCCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctc                                                                                                  >  1:573735/1‑140 (MQ=255)                                    aGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTAt                                                                                               >  2:776944/1‑140 (MQ=255)                                    aGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGAGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTAt                                                                                               >  2:299151/1‑140 (MQ=255)                                     gCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTAtt                                                                                              >  1:1409493/1‑140 (MQ=255)                                      cTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGAAAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTa                                                                                             <  1:345841/140‑1 (MQ=255)                                       ttCCGTTTGCTTCTTGATTCATTATCTCGGGGGCGATCCCCCACTTTTTCGTGCAACGCGTTTTTAACAAAAAAGAAAAAAATATCAACCACAAATAGGCAGAAAAAAAAGCTCTCAATAATGTCCCGCCGCTCTATTAc                                                                                            <  2:398855/140‑1 (MQ=255)                                       ttCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTAc                                                                                            <  1:583977/140‑1 (MQ=255)                                       ttCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTAc                                                                                            <  1:583142/140‑1 (MQ=255)                                       ttCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTAc                                                                                            >  1:996662/1‑140 (MQ=255)                                          cGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGcc                                                                                         >  2:295466/1‑140 (MQ=255)                                            gTCGCCTCTTGCGTCATTATCTCGGTGGCGGTCTCCCACTTTTTAGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGCATAGGCCGAGCAAAACGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAt                                                                                       >  2:266321/1‑140 (MQ=255)                                            gTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAt                                                                                       >  1:896562/1‑140 (MQ=255)                                             tCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtt                                                                                      >  1:1487061/1‑140 (MQ=255)                                               gCCTCTTGCGTCATTATCTCGGTGGCGATCCCCCACTTTTTCGTGCAACGCGCTTTTGAAAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtttt                                                                                    <  1:1170921/140‑1 (MQ=255)                                                 ctctTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTAc                                                                                  <  2:18488/140‑1 (MQ=255)                                                 ctctTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTAc                                                                                  >  2:829171/1‑140 (MQ=255)                                                      gCGTCATTATCTGGGTGGCGATCCCCCCCTTTTTCGTGCAACGCGTTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCtgtg                                                                             <  2:1053908/140‑1 (MQ=255)                                                       cGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGa                                                                            >  2:607707/1‑140 (MQ=255)                                                           aTTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGcc                                                                        >  1:912288/1‑140 (MQ=255)                                                             tATCTCGGTGGCGTCCTCCCCTTTTTTCGTGCACCGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAACCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGt                                                                      <  1:1492057/140‑1 (MQ=255)                                                              aTCTCGGTGGCGATCCCCCACTTTTTCGTGCAACGCATTTTTAACAAAAAAGATAAAAATATCAACCCCGAATAGGCAGAAAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt                                                                     <  1:1199270/140‑1 (MQ=255)                                                                ctcGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTaa                                                                   >  2:1243994/1‑140 (MQ=255)                                                                   ggTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATcc                                                                >  2:1162364/1‑140 (MQ=255)                                                                    gTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCt                                                               >  2:762050/1‑140 (MQ=255)                                                                          aTCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgct                                                         >  1:321283/1‑140 (MQ=255)                                                                           tctcCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAACAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctc                                                        >  1:803505/1‑140 (MQ=255)                                                                           tctcCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctc                                                        >  2:684529/1‑140 (MQ=255)                                                                            cccccTCTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcg                                                       <  2:566499/138‑1 (MQ=255)                                                                              cccACTTTTTCGTGCAACGCGTTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcggc                                                     <  1:1077493/140‑1 (MQ=255)                                                                                  cTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGAAAAAAGCTCTCGATAATGCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGc                                                 <  1:1145155/140‑1 (MQ=255)                                                                                            caccgcgCTTTGGAAAAAAAAGATAAAAATATCAACCCCGAATAGGCAGAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGc                                       <  2:1347298/137‑1 (MQ=255)                                                                                             aaCGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGcc                                      >  2:353475/1‑140 (MQ=255)                                                                                               cgcgCTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCa                                    >  2:206137/1‑140 (MQ=255)                                                                                                 cgcTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACg                                  >  1:726269/1‑140 (MQ=255)                                                                                                  gcTTTTGACAAAAAAGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGt                                 <  1:684529/140‑1 (MQ=255)                                                                                                           aaaaaaGATAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGAt                        <  1:776944/140‑1 (MQ=255)                                                                                                           aaaaaaGAAAAAAATATCAATCACGAATGGGCAGACCAAAAAGCCCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGAt                        <  2:996662/140‑1 (MQ=255)                                                                                                           aaaaaaGAAAAAAATATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGAt                        <  2:1045782/140‑1 (MQ=255)                                                                                                                aaaaaaaaaTATCAATCACGAATGGGAAGGGCAAAAGGCTCTCGATAATGGCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGt                   <  2:1539778/136‑1 (MQ=255)                                                                                                                     aaaaTATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGaa              <  1:979495/140‑1 (MQ=255)                                                                                                                     aaaaTATCAATCACGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGaa              >  2:1462290/1‑140 (MQ=255)                                                                                                                      aaaTATCAATCACGAATAGGCAGAGAAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAAc             <  1:829171/140‑1 (MQ=255)                                                                                                                            caatcaCGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATc       <  1:267121/140‑1 (MQ=255)                                                                                                                            caatcaCGAATAGGCAGAGAAAAAGGCTCTCGATATTGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATc       <  1:1459769/140‑1 (MQ=255)                                                                                                                              atcaCGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCtg     >  1:1236290/1‑140 (MQ=255)                                                                                                                               tcaCGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCtgg    >  2:113603/1‑140 (MQ=255)                                                                                                                               tcaCGAATAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCtgg    <  1:1084848/140‑1 (MQ=255)                                                                                                                                 aCGAATAGGCAGAGAAAAACCCTCTCGAAACTGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCtggtg  <  2:679598/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTCCGGTCGCCTCTTGCGTCATTATCTCGGTGGCGATCTCCCACTTTTTCGTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTG  >  NC_000913/3375423‑3375690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑