Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,827	G→A	G115E (GGG→GAG)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,827	0	G	A	100.0%	285.6 / NA	85	G115E (GGG→GAG)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/48);  total (37/48)

ACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTC  >  NC_000913/3403694‑3403960                                                                                                                                      |                                                                                                                                      cccAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGa                                                                                                                                 <  2:204797/139‑1 (MQ=255)  ccAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAc                                                                                                                                <  2:265416/140‑1 (MQ=255)      cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgc                                                                                                                            >  1:1548954/1‑140 (MQ=255)      cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgc                                                                                                                            >  1:937969/1‑140 (MQ=255)      cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgc                                                                                                                            >  1:415114/1‑140 (MQ=255)      cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgc                                                                                                                            >  2:293888/1‑140 (MQ=255)       gAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgcg                                                                                                                           <  2:825475/140‑1 (MQ=255)       gAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGAcgcgcg                                                                                                                           <  1:1243968/140‑1 (MQ=255)          gATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTaa                                                                                                                        <  1:1062078/140‑1 (MQ=255)           aTCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTaaa                                                                                                                       >  2:1021359/1‑140 (MQ=255)               gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCaa                                                                                                                   <  1:1260084/140‑1 (MQ=255)               gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCaa                                                                                                                   <  2:1772/140‑1 (MQ=255)               gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCaa                                                                                                                   <  2:208455/140‑1 (MQ=255)               gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCaa                                                                                                                   <  1:454100/140‑1 (MQ=255)                 gTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATg                                                                                                                 <  2:1359640/140‑1 (MQ=255)                 gTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATg                                                                                                                 <  2:937832/140‑1 (MQ=255)                 gTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATg                                                                                                                 <  1:723020/140‑1 (MQ=255)                    ttCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGAtt                                                                                                              <  2:910942/140‑1 (MQ=255)                           cGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACC‑‑‑GGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTg                                                                                                    >  2:710562/1‑140 (MQ=255)                             gTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACt                                                                                                     >  1:59685/1‑140 (MQ=255)                             gTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACt                                                                                                     <  2:1511270/140‑1 (MQ=255)                             gTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACt                                                                                                     <  1:527112/140‑1 (MQ=255)                                aggaggTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc                                                                                                  <  2:415114/140‑1 (MQ=255)                                       gtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                           >  1:1528027/1‑140 (MQ=255)                                       gtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                           <  1:1254913/140‑1 (MQ=255)                                       gtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTa                                                                                           <  1:1146880/140‑1 (MQ=255)                                        ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTAc                                                                                          >  1:175649/1‑140 (MQ=255)                                        ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTAc                                                                                          >  1:274451/1‑140 (MQ=255)                                        ttACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTAc                                                                                          >  2:769419/1‑140 (MQ=255)                                          aCTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCg                                                                                        <  1:894241/140‑1 (MQ=255)                                           cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGc                                                                                       <  2:529452/140‑1 (MQ=255)                                            tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc                                                                                      >  2:23175/1‑140 (MQ=255)                                             cACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCa                                                                                     >  2:1245495/1‑140 (MQ=255)                                             cACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCa                                                                                     >  1:185931/1‑140 (MQ=255)                                               ctggctggGGGGTTGGTGAAAGCCACGGGGGTGGGCTGGCGGGGCAGGTGCGGGTGAAAGGGGCCTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACGGCCGGTTTTACCGCCATg                                                                                   <  2:1456450/140‑1 (MQ=255)                                               ctggctggGGCGTTGGTGAAACCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                                                                   <  2:1320502/140‑1 (MQ=255)                                               ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                                                                   <  2:284920/140‑1 (MQ=255)                                               ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                                                                   <  1:1024741/140‑1 (MQ=255)                                               ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                                                                   <  1:1171889/140‑1 (MQ=255)                                               ccggctggGGCGTGGGTGAAAGCCACGGGGGTGGGCTGGGGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTGGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                                                                   <  2:1198965/138‑1 (MQ=255)                                                tggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGc                                                                                  >  1:1472809/1‑140 (MQ=255)                                                 ggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCt                                                                                 <  1:834992/140‑1 (MQ=255)                                                  gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtg                                                                                >  2:1517949/1‑140 (MQ=255)                                                  gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtg                                                                                >  1:1148262/1‑140 (MQ=255)                                                    tggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtgtg                                                                              >  2:1284101/1‑140 (MQ=255)                                                    tggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtgtg                                                                              >  2:640094/1‑140 (MQ=255)                                                     ggggCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtgtgt                                                                             >  2:220513/1‑140 (MQ=255)                                                            tGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATgg                                                                      >  1:309096/1‑140 (MQ=255)                                                            tGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATgg                                                                      >  2:40679/1‑140 (MQ=255)                                                            tGGTGAAAACCACTGGGGCGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCCACAGCTGGACGCGCGGAAAGCAATGAGCATCGGCACTGCCGGTTTTACCGCCCAGCTGAGTGTGATgg                                                                      >  2:674254/1‑140 (MQ=255)                                                                   aaCCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGa                                                               <  1:444723/140‑1 (MQ=255)                                                                      cACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGa                                                            >  2:762112/1‑140 (MQ=255)                                                                        cTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATg                                                          >  1:122980/1‑140 (MQ=255)                                                                           gagTGGGCTGTGGGAGCAGGCGCAAGTAAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGTTGGACGCGTGTAAACCAATGATTATCGGTTGGGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCg                                                       <  2:189106/138‑1 (MQ=255)                                                                              tGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGgtg                                                    >  2:951509/1‑140 (MQ=255)                                                                                ggCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGtt                                                  <  1:318395/140‑1 (MQ=255)                                                                                ggCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGtt                                                  >  2:398357/1‑140 (MQ=255)                                                                                     gCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGccc                                             <  1:951509/140‑1 (MQ=255)                                                                                     gCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGccc                                             >  1:1323173/1‑140 (MQ=255)                                                                                       ggAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGc                                           <  1:710562/140‑1 (MQ=255)                                                                                        gAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGGGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCa                                          <  1:374844/140‑1 (MQ=255)                                                                                        gAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCa                                          <  1:23175/140‑1 (MQ=255)                                                                                          gCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAgg                                        >  1:39945/1‑140 (MQ=255)                                                                                           cAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGa                                       >  2:957693/1‑140 (MQ=255)                                                                                           cAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGa                                       <  2:122980/140‑1 (MQ=255)                                                                                           cAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGa                                       <  2:1472809/140‑1 (MQ=255)                                                                                            gggCGAGAGTGAAGGGTGACTGGCTGGTGGCCATGCCGCAAGGGCGGGCCGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTCCCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                                      <  2:274451/139‑1 (MQ=255)                                                                                             ggCGGGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACg                                     <  1:1021359/140‑1 (MQ=255)                                                                                                gcgAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACgggg                                  <  1:40679/140‑1 (MQ=255)                                                                                                 cgAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGga                                 <  2:175649/140‑1 (MQ=255)                                                                                                   aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGgaga                               <  1:293888/140‑1 (MQ=255)                                                                                                   aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGgaga                               >  1:689623/1‑140 (MQ=255)                                                                                                   aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGgaga                               <  1:1517949/140‑1 (MQ=255)                                                                                                        aaGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTc                          >  1:359159/1‑140 (MQ=255)                                                                                                        aaGGTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTc                          <  1:769419/140‑1 (MQ=255)                                                                                                           gTGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTg                       <  1:1245495/140‑1 (MQ=255)                                                                                                            tGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGa                      <  1:1284101/140‑1 (MQ=255)                                                                                                            tGACTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGa                      <  1:640094/140‑1 (MQ=255)                                                                                                              gctggctggTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACg                    <  2:447392/139‑1 (MQ=255)                                                                                                              aCTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACg                    >  1:737551/1‑140 (MQ=255)                                                                                                              aCTGGCTGGTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACg                    >  1:226332/1‑140 (MQ=255)                                                                                                                 ggctggTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGt                 <  2:1528027/140‑1 (MQ=255)                                                                                                                  gctggTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTg                >  1:1274253/1‑140 (MQ=255)                                                                                                                   gtggTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGc               <  2:1288197/139‑1 (MQ=255)                                                                                                                      gTTGCCATGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAg            >  2:401411/1‑140 (MQ=255)                                                                                                                             tGCCGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGc     <  1:762112/140‑1 (MQ=255)                                                                                                                               ccGCAAGAGCTGGACGCGCGTAAAGCAGTGATTATCGGTACTGCCGGCTTTACCGCCATGCTGTGCGCGATGGCGCTGGAAGATGCCGGGGTTCGCCCGAAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGt   >  2:627988/1‑140 (MQ=255)                                                                                                                                cGCAAGAGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTc  >  1:1298761/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      ACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTGACGGGTGCCAGTGGTGGCGTC  >  NC_000913/3403694‑3403960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑