Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,445,283 T→C A105A (GCA→GCG)  rplR ← 50S ribosomal subunit protein L18

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,445,2830TC99.0% 369.3 / ‑4.7 100A105A (GCA→GCG) rplR50S ribosomal subunit protein L18
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (44/55);  total (44/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 106 total aligned reads displayed.

ACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGTGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAGTTGTT  >  NC_000913/3445146‑3445419
                                                                                                                                         |                                                                                                                                        
accaccTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAgcgc                                                                                                                                        >  1:795646/1‑140 (MQ=255)
 ccaccTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGcc                                                                                                                                       <  1:386745/140‑1 (MQ=255)
     cTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGa                                                                                                                                   >  1:1020053/1‑140 (MQ=255)
     cTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGa                                                                                                                                   <  1:851951/140‑1 (MQ=255)
     cTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGa                                                                                                                                   <  2:1191408/140‑1 (MQ=255)
      tttAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGac                                                                                                                                  <  1:31144/140‑1 (MQ=255)
       ttAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTGCACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGaca                                                                                                                                 <  1:1111442/140‑1 (MQ=255)
       ttAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGaca                                                                                                                                 <  2:861724/140‑1 (MQ=255)
           cGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGAc                                                                                                                             <  2:352714/140‑1 (MQ=255)
             gTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCa                                                                                                                           <  1:523742/140‑1 (MQ=255)
             gTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCa                                                                                                                           <  1:19216/140‑1 (MQ=255)
              ttttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCAt                                                                                                                          <  1:157611/140‑1 (MQ=255)
               tttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATg                                                                                                                         >  1:109701/1‑140 (MQ=255)
               tttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATg                                                                                                                         <  2:374550/140‑1 (MQ=255)
               tttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATg                                                                                                                         >  2:576128/1‑140 (MQ=255)
               tttAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATg                                                                                                                         >  1:212639/1‑140 (MQ=255)
                    aTACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATAtt                                                                                                                    <  2:330851/140‑1 (MQ=255)
                    aTACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATAtt                                                                                                                    <  1:1062113/140‑1 (MQ=255)
                     tACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTg                                                                                                                   <  1:469742/140‑1 (MQ=255)
                     tACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTg                                                                                                                   <  1:950911/140‑1 (MQ=255)
                       cgcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGa                                                                                                                 >  2:102350/1‑140 (MQ=255)
                        gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  1:1492089/1‑140 (MQ=255)
                        gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  2:496442/1‑140 (MQ=255)
                        gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  1:1173768/1‑140 (MQ=255)
                        gcgGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGaa                                                                                                                >  1:1175877/1‑140 (MQ=255)
                            tttCCCGCGATCAGCTATTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCGTCTTACTCCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGGCCATGATATTGGAACCCg                                                                                                            <  2:1188683/140‑1 (MQ=255)
                             ttACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCgg                                                                                                           <  2:968589/140‑1 (MQ=255)
                              tACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGa                                                                                                          <  1:712003/140‑1 (MQ=255)
                               gccGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAGGGGAGCCATCTTACCCCTCTAGCTTAGACCTAAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGATCCCGGaa                                                                                                         <  2:301819/139‑1 (MQ=255)
                               aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  2:68331/140‑1 (MQ=255)
                               aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  2:543197/140‑1 (MQ=255)
                               aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  2:516534/140‑1 (MQ=255)
                               aCCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGaa                                                                                                         <  1:81849/140‑1 (MQ=255)
                                ccGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAAc                                                                                                        <  1:18228/140‑1 (MQ=255)
                                   cgATCAGCTTTTCCTGCTGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTTCCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGt                                                                                                     <  1:1546416/140‑1 (MQ=255)
                                    gATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTc                                                                                                    <  2:212639/140‑1 (MQ=255)
                                    gATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTc                                                                                                    <  1:1407952/140‑1 (MQ=255)
                                     aTCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   <  2:238040/140‑1 (MQ=255)
                                     aTCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCa                                                                                                   <  2:123598/140‑1 (MQ=255)
                                      tCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCaa                                                                                                  >  1:402939/1‑140 (MQ=255)
                                       cAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCaaa                                                                                                 <  1:148375/140‑1 (MQ=255)
                                        aGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAg                                                                                                >  2:1139051/1‑140 (MQ=255)
                                              tCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACa                                                                                          >  1:1219393/1‑140 (MQ=255)
                                               ccTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGCCAAAGGATACAt                                                                                         >  1:1325460/1‑140 (MQ=255)
                                                   cAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCttt                                                                                     >  2:1087815/1‑140 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGa                                                                                   <  2:1477373/140‑1 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGa                                                                                   <  2:1250503/140‑1 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGa                                                                                   >  1:376909/1‑140 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGa                                                                                   >  1:562482/1‑140 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGa                                                                                   <  2:580736/140‑1 (MQ=255)
                                                     gTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTT‑GAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGAt                                                                                  >  1:1415144/1‑140 (MQ=255)
                                                      ttCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGAt                                                                                  >  1:62317/1‑140 (MQ=255)
                                                       tCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATg                                                                                 <  1:576128/140‑1 (MQ=255)
                                                        cGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGc                                                                                >  2:303088/1‑140 (MQ=255)
                                                         gCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATGGGAACCCGGAACGGTCAAAGGATACATCTTTGATGcc                                                                               >  1:1230248/1‑140 (MQ=255)
                                                         gCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGACTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGcc                                                                               <  2:701085/140‑1 (MQ=255)
                                                              cTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCttttt                                                                          >  2:28625/1‑140 (MQ=255)
                                                                    tttCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCagag                                                                    <  1:341186/140‑1 (MQ=255)
                                                                      tCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAgcg                                                                  <  2:1020053/140‑1 (MQ=255)
                                                                         aTGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGt                                                               >  1:1014129/1‑140 (MQ=255)
                                                                         aTGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGt                                                               >  1:1217928/1‑140 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGTGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  2:76000/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  1:114307/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  1:1139051/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  1:1096830/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  1:730468/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  2:1175877/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  1:545005/140‑1 (MQ=255)
                                                                                ccATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTAACGTGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAGCGGTCAAAGGATACATCTTTGATGTCTTTTTCCAGAGCGCGTTCAGCGa                                                        <  2:463885/140‑1 (MQ=255)
                                                                                    cTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGc                                                    >  2:633762/1‑140 (MQ=255)
                                                                                     ttACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCt                                                   <  2:731530/140‑1 (MQ=255)
                                                                                          ccTCTACCAACGAAGCGAAGGCCAGCTTGACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATGCATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTAcc                                              <  2:861242/140‑1 (MQ=255)
                                                                                            tctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCca                                            <  2:799152/140‑1 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGTCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  1:457510/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  2:907949/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  1:813668/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  2:5960/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  1:1138836/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  2:818961/1‑140 (MQ=255)
                                                                                             ctACCTTAGAACTGAAGGCCAACTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcac                                           >  2:914043/1‑140 (MQ=255)
                                                                                              tACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcaca                                          >  2:293584/1‑140 (MQ=255)
                                                                                              tACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCcaca                                          >  1:1198364/1‑140 (MQ=255)
                                                                                                     gAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAg                                   >  1:215174/1‑140 (MQ=255)
                                                                                                          gAAGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcgcg                              <  1:97784/140‑1 (MQ=255)
                                                                                                           aaGGCCAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGt                             >  2:773901/1‑140 (MQ=255)
                                                                                                           aaGGCCAGATTCACGGGCAGCATCTGCCAGCGCCTGGACCCGACCATGATATTGGAACCCGGAGCGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCcgcgg                             >  2:583846/1‑139 (MQ=255)
                                                                                                            aGGCCACCATCTCGGGCGGCATCTCCTAGCGCCGGGACACGACCATGATATTGGACCCCGGAACGGTCAAAGGATGCATCTTTGATGCCTTTTTCCAGAGCGCGTTCACCGACAGCTTTACCCACAGCTGCAGCCGCGTc                            <  2:429306/140‑1 (MQ=255)
                                                                                                                cAGCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTg                        >  1:333044/1‑140 (MQ=255)
                                                                                                                  gCTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGCCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGtt                      <  2:1325460/140‑1 (MQ=255)
                                                                                                                   cTTCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTa                     >  1:521804/1‑140 (MQ=255)
                                                                                                                    ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGATCCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  1:515379/140‑1 (MQ=255)
                                                                                                                    ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATGCATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTTCCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  1:985536/140‑1 (MQ=255)
                                                                                                                    ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  2:456683/140‑1 (MQ=255)
                                                                                                                    ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  2:1219393/140‑1 (MQ=255)
                                                                                                                    ttCACGGGCAGCATCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTAc                    <  1:102350/140‑1 (MQ=255)
                                                                                                                              gcaTCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACtt          >  2:1367714/1‑140 (MQ=255)
                                                                                                                                 tCTGCCAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAg       >  1:233119/1‑140 (MQ=255)
                                                                                                                                 tCTGCCAGCGCCTGGACACGACCATGATATTGCAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAg       >  2:115927/1‑140 (MQ=255)
                                                                                                                                      cAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAgttgtt  <  2:795646/140‑1 (MQ=255)
                                                                                                                                      cAGCGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAgttgtt  <  1:496442/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTTACACCTCTACCTTAGAACTGAAGGCCAGCTTCACGGGCAGCATCTGCCAGTGCCTGGACACGACCATGATATTGGAACCCGGAACGGTCAAAGGATACATCTTTGATGCCTTTTTCCAGAGCGCGTTCAGCGACAGCTTTACCCACAGCTGCAGCCGCGTCTTTGTTACCGGTGTACTTCAGTTGTT  >  NC_000913/3445146‑3445419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑