Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,884,188	T→C	intergenic (+82/‑148)	ysdD → / → rpmH	protein YsdD/50S ribosomal subunit protein L34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,884,188	0	T	C	100.0%	339.9 / NA	91	intergenic (+82/‑148)	ysdD/rpmH	protein YsdD/50S ribosomal subunit protein L34
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/43);  total (48/43)

TCGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTA  >  NC_000913/3884052‑3884322                                                                                                                                         |                                                                                                                                       tCGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCaaa                                                                                                                                     <  2:322249/140‑1 (MQ=255) tCGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCaaa                                                                                                                                     <  2:1095679/140‑1 (MQ=255)  cGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAAt                                                                                                                                    <  2:1258649/140‑1 (MQ=255)  cGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAAt                                                                                                                                    <  2:1425461/140‑1 (MQ=255)  cGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAAt                                                                                                                                    <  2:311150/140‑1 (MQ=255)    aTCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATaa                                                                                                                                  <  1:542729/140‑1 (MQ=255)    aTCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATaa                                                                                                                                  <  1:719808/140‑1 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       >  2:1496703/1‑140 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       <  2:569561/140‑1 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       <  2:577671/140‑1 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       <  1:1293554/140‑1 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       >  2:1513324/1‑140 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       >  2:913243/1‑140 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       <  1:1179014/140‑1 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       >  1:1126674/1‑140 (MQ=255)               aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAAt                                                                                                                       <  1:33710/140‑1 (MQ=255)                 ccAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTg                                                                                                                     >  2:1326175/1‑140 (MQ=255)                  cAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGa                                                                                                                    >  1:1071365/1‑140 (MQ=255)                   aGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCCCCCCGCGCGCCATGCTACATTCCACTGGGGCGAGGGCGTACATTTTCCCTGCGAACAGGTGCGGCAAAGCGCGGCAAATGAGGAAAGAGAATTGAc                                                                                                                   >  2:1373017/1‑140 (MQ=255)                   aGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGAc                                                                                                                   >  1:1040094/1‑140 (MQ=255)                   aGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGAc                                                                                                                   >  1:581128/1‑140 (MQ=255)                       cGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCg                                                                                                               <  1:14649/140‑1 (MQ=255)                       cGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCg                                                                                                               <  2:318314/140‑1 (MQ=255)                       cGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCg                                                                                                               <  2:511927/140‑1 (MQ=255)                       cGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCg                                                                                                               <  2:782336/140‑1 (MQ=255)                       cGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCg                                                                                                               <  1:451631/140‑1 (MQ=255)                       cGATCCTTGCGCTTTACCCAGCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTATCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGTTTCCg                                                                                                               <  2:26716/140‑1 (MQ=255)                         gtCCTTGCGCATTAGCCATCAGCGCGTATAAGCCTCTACCCGGCGCGCCATGCGGGTTTCCACTGGTGTGAGGGAGTACGTTTTCCCTGCGTTAAGGTGCGGACAAGTGCGGCAAATAAGGAAAGAGAATTGTCACCGGa                                                                                                             <  2:638974/139‑1 (MQ=255)                         aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGa                                                                                                             >  1:567043/1‑140 (MQ=255)                         aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGa                                                                                                             <  1:610097/140‑1 (MQ=255)                         aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGa                                                                                                             <  2:439294/140‑1 (MQ=255)                          tCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAg                                                                                                            >  1:284951/1‑140 (MQ=255)                            cTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAgtg                                                                                                          <  2:34233/140‑1 (MQ=255)                                 gcTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACaa                                                                                                     <  2:1316178/140‑1 (MQ=255)                                     tACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAattat                                                                                                 >  2:785525/1‑140 (MQ=255)                                      aCCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAattatt                                                                                                <  1:384748/140‑1 (MQ=255)                                      aCCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAattatt                                                                                                <  1:1028045/140‑1 (MQ=255)                                         cATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACa                                                                                             <  2:244474/140‑1 (MQ=255)                                            cAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGAGTACAATTATTACAATc                                                                                          >  1:770153/1‑140 (MQ=255)                                             aGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATcc                                                                                         >  2:1071963/1‑140 (MQ=255)                                               cccGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCgg                                                                                       >  1:160401/1‑140 (MQ=255)                                               cccGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCgg                                                                                       <  2:473091/140‑1 (MQ=255)                                               cccGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCgg                                                                                       <  2:1050846/140‑1 (MQ=255)                                                ccGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGc                                                                                      <  1:273675/140‑1 (MQ=255)                                                  gTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCct                                                                                    <  2:1508133/140‑1 (MQ=255)                                                     taATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCtt                                                                                 >  1:287657/1‑140 (MQ=255)                                                        tcctccACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTaa                                                                              <  2:168595/140‑1 (MQ=255)                                                        tcctccACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTaa                                                                              <  2:1261256/140‑1 (MQ=255)                                                         cctccACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAAt                                                                             <  2:335195/140‑1 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  1:268240/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  2:1275277/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  1:1522996/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  2:733769/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  2:875602/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  1:566396/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         >  1:173290/1‑140 (MQ=255)                                                             cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGCCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                         <  1:62117/140‑1 (MQ=255)                                                              aCCCGGCGCGCCATGCTGGTTTCCACTGGTGTGGGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAccc                                                                        >  2:227540/1‑140 (MQ=255)                                                               cccGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCa                                                                       <  1:747722/140‑1 (MQ=255)                                                                ccGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCAt                                                                      >  1:408966/1‑140 (MQ=255)                                                                  ggCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATgg                                                                    >  2:1474722/1‑140 (MQ=255)                                                                  ggCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATgg                                                                    >  1:129906/1‑140 (MQ=255)                                                                  ggCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATgg                                                                    >  1:1531637/1‑140 (MQ=255)                                                                    cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCt                                                                  >  1:102589/1‑140 (MQ=255)                                                                    cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGACCTCTTTAATCACCCATGGCt                                                                  >  2:947754/1‑140 (MQ=255)                                                                     gcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCtt                                                                 <  1:1369454/140‑1 (MQ=255)                                                                     gcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCtt                                                                 <  1:785525/140‑1 (MQ=255)                                                                            gCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTc                                                          <  2:284951/140‑1 (MQ=255)                                                                             cTGGTTTCCACTGGTGGGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTcc                                                         >  2:951833/1‑140 (MQ=255)                                                                                     cACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTc                                                 <  2:567043/140‑1 (MQ=255)                                                                                             tgAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTcg                                         >  2:511331/1‑140 (MQ=255)                                                                                             tgAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGCGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTcg                                         >  2:20313/1‑140 (MQ=255)                                                                                                    gTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                                  >  1:661215/1‑140 (MQ=255)                                                                                                    gTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                                  >  1:453645/1‑140 (MQ=255)                                                                                                    gTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                                  >  2:46772/1‑140 (MQ=255)                                                                                                    gTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                                  >  1:1411976/1‑140 (MQ=255)                                                                                                    gTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                                  >  2:389668/1‑140 (MQ=255)                                                                                                     tACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGatat                                 >  2:296942/1‑140 (MQ=255)                                                                                                           ttCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATa                           <  2:1522996/140‑1 (MQ=255)                                                                                                            tCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATaa                          >  1:434705/1‑140 (MQ=255)                                                                                                            tCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATaa                          <  1:1409794/140‑1 (MQ=255)                                                                                                            tCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATaa                          >  1:125637/1‑140 (MQ=255)                                                                                                            tCCCTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATaa                          <  1:1275277/140‑1 (MQ=255)                                                                                                              ccTGCGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAg                        >  1:243951/1‑140 (MQ=255)                                                                                                                  cGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCAt                    >  2:1492247/1‑140 (MQ=255)                                                                                                                  cGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCAt                    >  1:1203877/1‑140 (MQ=255)                                                                                                                  cGAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCAt                    >  1:567983/1‑140 (MQ=255)                                                                                                                   gAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCAtt                   <  2:1126674/140‑1 (MQ=255)                                                                                                                   gAAAAGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCAtt                   >  2:1306864/1‑140 (MQ=255)                                                                                                                    aaaaGGTGCGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTCTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTg                  >  2:540543/1‑140 (MQ=255)                                                                                                                            cGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCTCACATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAtt          <  1:951833/140‑1 (MQ=255)                                                                                                                            cGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAtt          >  2:187377/1‑140 (MQ=255)                                                                                                                            cGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAtt          >  1:119137/1‑140 (MQ=255)                                                                                                                            cGGAAAAGCGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATCACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAtt          <  1:1373017/140‑1 (MQ=255)                                                                                                                                 aaGTGCGGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGt     <  1:20313/140‑1 (MQ=255)                                                                                                                                    cgcgGCAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTa  >  1:543980/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TCGATCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTA  >  NC_000913/3884052‑3884322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑