BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,934,828	A→G	M351V (ATG→GTG)	rbsA →	ribose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,934,828	0	A	G	100.0%	425.6 / NA	112	M351V (ATG→GTG)	rbsA	ribose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (72/40); total (72/40)

Only 100 of 114 total aligned reads displayed.

CAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACATGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCT  >  NC_000913/3934692‑3934964

cAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACgtgt                                                                                                                                       >  2:132157/1‑140 (MQ=255)

cAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACgtgt                                                                                                                                       >  1:503602/1‑140 (MQ=255)

 aGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTc                                                                                                                                      <  2:492870/140‑1 (MQ=255)

        aCGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGAAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACa                                                                                                                               >  2:1137609/1‑140 (MQ=255)

           tCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCGAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAgcg                                                                                                                            <  2:142697/140‑1 (MQ=255)

           tCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAgcg                                                                                                                            <  2:683021/140‑1 (MQ=255)

                cTGGATCGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAgcgctgcg                                                                                                                       <  2:1126293/140‑1 (MQ=255)

                 tGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAgcgctgcgc                                                                                                                      <  1:1115176/140‑1 (MQ=255)

                 tGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAgcgctgcgc                                                                                                                      <  1:872929/140‑1 (MQ=255)

                   gATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGATGACAGCGCTGCGCTa                                                                                                                    >  2:1269349/1‑140 (MQ=255)

                    aTGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTAc                                                                                                                   >  1:890818/1‑140 (MQ=255)

                        gCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCa                                                                                                               >  2:1517620/1‑140 (MQ=255)

                         cATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAg                                                                                                              >  1:1039167/1‑140 (MQ=255)

                         cATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAg                                                                                                              >  1:612074/1‑140 (MQ=255)

                                  gTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTgg                                                                                                     >  1:530580/1‑140 (MQ=255)

                                  gTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTgg                                                                                                     >  2:9047/1‑140 (MQ=255)

                                  gTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTgg                                                                                                     >  2:1215726/1‑140 (MQ=255)

                                  gTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCCCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTgg                                                                                                     >  1:196762/1‑140 (MQ=255)

                                  gTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATCGCGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTgg                                                                                                     >  2:405113/1‑140 (MQ=255)

                                     tcccGTGCGCCGCAGGATGGCCTGGCAAGCGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTggcgg                                                                                                  <  2:862104/139‑1 (MQ=255)

                                     aCCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTggcgg                                                                                                  <  1:1170053/140‑1 (MQ=255)

                                     aCCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTggcgg                                                                                                  <  1:1050921/140‑1 (MQ=255)

                                      cccGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTggcggc                                                                                                 <  2:1039167/140‑1 (MQ=255)

                                         gTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGt                                                                                              <  1:1043061/140‑1 (MQ=255)

                                            cACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTg                                                                                           <  2:312677/140‑1 (MQ=255)

                                                 cAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCa                                                                                      >  1:1544369/1‑140 (MQ=255)

                                                 cAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCa                                                                                      <  1:1420193/140‑1 (MQ=255)

                                                 cAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCa                                                                                      <  2:778412/140‑1 (MQ=255)

                                                    gATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGc                                                                                   >  2:1045504/1‑140 (MQ=255)

                                                      tGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCg                                                                                 <  1:132157/140‑1 (MQ=255)

                                                        gCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGAt                                                                               <  2:710143/140‑1 (MQ=255)

                                                        gCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGAt                                                                               <  2:767768/140‑1 (MQ=255)

                                                         ccTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATg                                                                              >  1:1460976/1‑140 (MQ=255)

                                                         ccTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATg                                                                              >  1:783471/1‑140 (MQ=255)

                                                             gCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAca                                                                          >  1:170029/1‑140 (MQ=255)

                                                              cAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcag                                                                         <  2:530580/140‑1 (MQ=255)

                                                              cAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcag                                                                         >  2:648366/1‑140 (MQ=255)

                                                              cAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcag                                                                         <  2:957161/140‑1 (MQ=255)

                                                              cAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcag                                                                         <  1:1356476/140‑1 (MQ=255)

                                                              cAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcag                                                                         >  1:1352968/1‑140 (MQ=255)

                                                                aaCGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAcagca                                                                       <  2:635426/140‑1 (MQ=255)

                                                                  cGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAgg                                                                     >  1:1139343/1‑140 (MQ=255)

                                                                  cGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAgg                                                                     <  1:1215726/140‑1 (MQ=255)

                                                                  cGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGATTGAAGCATGCCGATGAACAGCAgg                                                                     >  2:859311/1‑140 (MQ=255)

                                                                     cATTGTGTATATCTCCGAAGACCGTAAACGTGGCGGTTTAGTGGTGGGCATGTCAGTGAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGACAGTTTGAAGCATGCCGATGAACAGCAGACtg                                                                  <  2:112752/140‑1 (MQ=255)

                                                                       ttGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTATCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCtgtg                                                                >  2:479130/1‑140 (MQ=255)

                                                                          tgtATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTgtgagt                                                             <  2:703943/140‑1 (MQ=255)

                                                                               atCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGAttt                                                        >  1:119680/1‑140 (MQ=255)

                                                                               atCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGAttt                                                        >  2:548801/1‑140 (MQ=255)

                                                                                 ctcCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCa                                                      >  1:14347/1‑140 (MQ=255)

                                                                                 ctcCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCa                                                      >  2:1322322/1‑140 (MQ=255)

                                                                                 ctcCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCa                                                      >  1:1284759/1‑140 (MQ=255)

                                                                                   ccGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCAtt                                                    <  2:890818/140‑1 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  2:1536335/1‑140 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  1:1299031/1‑140 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  1:94487/1‑140 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  1:493634/1‑140 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  1:663276/1‑140 (MQ=255)

                                                                                    cGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTc                                                   >  1:1070373/1‑140 (MQ=255)

                                                                                      aaGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGt                                                 >  2:91567/1‑140 (MQ=255)

                                                                                      aaGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGt                                                 >  2:291706/1‑140 (MQ=255)

                                                                                           cGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGtt                                            >  1:427321/1‑140 (MQ=255)

                                                                                           cGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGtt                                            >  2:10297/1‑140 (MQ=255)

                                                                                            gTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGttt                                           <  2:503602/140‑1 (MQ=255)

                                                                                                   tGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGa                                    >  2:591066/1‑140 (MQ=255)

                                                                                                    gACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaa                                   >  1:952442/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAAGAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  2:1445397/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  1:1486477/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  1:1504088/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  1:51011/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  2:1480792/1‑140 (MQ=255)

                                                                                                     aCGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGaaa                                  >  1:188/1‑140 (MQ=255)

                                                                                                          ttAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTcc                             >  2:1496288/1‑140 (MQ=255)

                                                                                                          ttAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTcc                             <  2:1284759/140‑1 (MQ=255)

                                                                                                           tAGTGTTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCg                            >  1:33027/1‑140 (MQ=255)

                                                                                                              tgtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCg                         >  2:425419/1‑140 (MQ=255)

                                                                                                              tgtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCg                         >  2:624867/1‑140 (MQ=255)

                                                                                                               gtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGa                        <  1:266384/140‑1 (MQ=255)

                                                                                                               gtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGa                        >  1:563670/1‑140 (MQ=255)

                                                                                                               gtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGa                        <  1:859311/140‑1 (MQ=255)

                                                                                                               gtTGGGCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGa                        <  2:196762/140‑1 (MQ=255)

                                                                                                                  gggCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATgg                     >  1:1499524/1‑140 (MQ=255)

                                                                                                                   ggCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGa                    >  1:245665/1‑140 (MQ=255)

                                                                                                                   ggCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGa                    >  1:1525658/1‑140 (MQ=255)

                                                                                                                   ggCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGa                    >  1:606105/1‑140 (MQ=255)

                                                                                                                   ggCATGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGa                    >  2:700661/1‑140 (MQ=255)

                                                                                                                      aTGTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACa                 <  2:1392547/140‑1 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               >  2:1499766/1‑140 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               >  1:657264/1‑140 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               >  1:69769/1‑140 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               >  1:431489/1‑140 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               >  2:1116510/1‑140 (MQ=255)

                                                                                                                        gTCAGTAAAAGAGAACGTGTCGATGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAgg               <  1:1269349/140‑1 (MQ=255)

                                                                                                                          cAGTAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCa             >  1:979478/1‑140 (MQ=255)

                                                                                                                             tAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAAtt          <  1:405113/140‑1 (MQ=255)

                                                                                                                             tAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAAtt          >  2:700236/1‑140 (MQ=255)

                                                                                                                             tAAAAGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAAtt          <  2:947254/140‑1 (MQ=255)

                                                                                                                                aaGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGt       <  1:1517620/140‑1 (MQ=255)

                                                                                                                                aaGAGAACGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGt       <  2:612074/140‑1 (MQ=255)

                                                                                                                                     aaCGTGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCt  >  1:869313/1‑140 (MQ=255)

CAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCATTGTGTATATCTCCGAAGACCGTAAACGTGACGGTTTAGTGTTGGGCATGTCAGTAAAAGAGAACATGTCGCTGACAGCGCTGCGCTACTTCAGCCGCGCTGGCGGCAGTTTGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCT  >  NC_000913/3934692‑3934964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`