Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,152,699	C→T	V134I (GTC→ATC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,152,699	0	C	T	100.0%	291.3 / NA	83	V134I (GTC→ATC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/33);  total (50/33)

GGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTG  >  NC_000913/4152567‑4152834                                                                                                                                     |                                                                                                                                        ggCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTcc                                                                                                                                  >  1:1333369/1‑140 (MQ=255)    gACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCcagc                                                                                                                               <  1:481756/140‑1 (MQ=255)       gCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCcagcgac                                                                                                                            <  1:831620/140‑1 (MQ=255)          tCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCcagcgacagc                                                                                                                         <  2:1175206/140‑1 (MQ=255)            aGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCcagcgacagcga                                                                                                                       >  1:347857/1‑140 (MQ=255)            aGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCcagcgacagcga                                                                                                                       >  2:768700/1‑140 (MQ=255)                 gTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCa                                                                                                                  <  2:1232752/140‑1 (MQ=255)                   tgtgTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACt                                                                                                                >  1:915859/1‑140 (MQ=255)                       ttCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCtt                                                                                                            >  1:1078685/1‑140 (MQ=255)                       ttCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCtt                                                                                                            >  1:55805/1‑140 (MQ=255)                          gTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCttttt                                                                                                         <  2:991930/140‑1 (MQ=255)                           tAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCtttttt                                                                                                        >  2:1307578/1‑140 (MQ=255)                             gTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGa                                                                                                      <  1:1462120/140‑1 (MQ=255)                               cAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATg                                                                                                    <  1:1097445/140‑1 (MQ=255)                                aGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATgg                                                                                                   >  1:342304/1‑140 (MQ=255)                                aGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATgg                                                                                                   >  1:1051833/1‑140 (MQ=255)                                 gCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGgt                                                                                                  >  2:1364939/1‑140 (MQ=255)                                  cGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGgtg                                                                                                 >  2:123660/1‑140 (MQ=255)                                   gATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGgtgt                                                                                                >  2:609114/1‑140 (MQ=255)                                     tatCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCt                                                                                              <  2:899795/140‑1 (MQ=255)                                     tatCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCt                                                                                              <  2:1498920/140‑1 (MQ=255)                                          ttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCt                                                                                         <  2:347857/140‑1 (MQ=255)                                          ttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCt                                                                                         <  2:1038792/140‑1 (MQ=255)                                                tCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTc                                                                                   >  1:839040/1‑140 (MQ=255)                                                     cTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCt                                                                              >  2:388558/1‑140 (MQ=255)                                                       gTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTgg                                                                            >  2:1128403/1‑140 (MQ=255)                                                          ttAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTT‑GGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGtttt                                                                        <  2:1547006/140‑1 (MQ=255)                                                               cAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAg                                                                    <  2:111226/140‑1 (MQ=255)                                                                 ggCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGtt                                                                  <  2:1333369/140‑1 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCCGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:901540/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGTGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:1438017/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  1:206786/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  1:983355/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  1:110072/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:1144267/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  1:657408/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  1:1465903/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:591661/1‑140 (MQ=255)                                                                  gCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGCAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:693447/1‑140 (MQ=255)                                                                  gCGTTCAATTCCACCAGTTTGTGGAACAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGttt                                                                 >  2:704548/1‑140 (MQ=255)                                                                     ttCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACg                                                              <  1:100881/140‑1 (MQ=255)                                                                     ttCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACg                                                              <  2:331635/140‑1 (MQ=255)                                                                     ttCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACg                                                              <  2:968370/140‑1 (MQ=255)                                                                     ttCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACg                                                              <  2:1051833/140‑1 (MQ=255)                                                                       cACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCa                                                            <  1:123660/140‑1 (MQ=255)                                                                            ccaccaTTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTg                                                       >  2:1028604/1‑140 (MQ=255)                                                                               ccaTTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCgg                                                    >  2:470487/1‑140 (MQ=255)                                                                               ccaTTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGGGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGCGCgg                                                    >  2:374715/1‑140 (MQ=255)                                                                                  ttttGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCg                                                 >  2:300696/1‑140 (MQ=255)                                                                                   tttGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGa                                                <  2:1465903/140‑1 (MQ=255)                                                                                    ttGTGGATCAGTGTACGACGGGTAATTTCGTTTGGGTGAGCCGTGAGGCTCAGTTTGAGCGACAGCGATTCCGCTGCTTTTTTGATGGTGTCTCCGCTCAGTTCCGGCTGGTTTTTCATTTCACGCAGGGTGCGGGCGAt                                               <  2:704498/140‑1 (MQ=255)                                                                                    ttGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGAt                                               <  2:3730/140‑1 (MQ=255)                                                                                    ttGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGAt                                               <  1:388558/140‑1 (MQ=255)                                                                                    ttGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGAt                                               <  1:1307578/140‑1 (MQ=255)                                                                                    tagtgGACCAGTGTACGTCGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTTTAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTACAGAGTACGTAGGGGGCGGGCGAt                                               <  2:198710/138‑1 (MQ=255)                                                                                              gtgtACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCggg                                     <  2:342304/140‑1 (MQ=255)                                                                                               tgtACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGt                                    >  2:1376462/1‑140 (MQ=255)                                                                                                   cgacgGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCt                                >  2:204144/1‑140 (MQ=255)                                                                                                      cgGGTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGc                             >  1:1018997/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  2:1304950/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:1192130/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:125592/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:1279798/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:182484/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  2:437018/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:612314/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:702940/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  2:1455971/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:814071/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:881419/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTCCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGc                          >  1:543657/1‑140 (MQ=255)                                                                                                         gTAATTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCACTCAGTTCCGGCTGGTTTTTCAGTTTACGCGGGGGGCGGGCGATAACTTCCGGGTTGCTGGCacc                          >  1:1539605/1‑138 (MQ=255)                                                                                                            aTTTCGGTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                       <  2:400011/140‑1 (MQ=255)                                                                                                                  gTTGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGTTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCttt                 <  2:308893/140‑1 (MQ=255)                                                                                                                   ttGGGTGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc                >  1:211710/1‑140 (MQ=255)                                                                                                                        tGAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGa           <  1:1028604/140‑1 (MQ=255)                                                                                                                         gAGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGaa          <  2:839040/140‑1 (MQ=255)                                                                                                                          aGCCGTGAGGATCAGTTCCAGCGACAGGGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGaaa         >  2:805023/1‑140 (MQ=255)                                                                                                                          aGCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGaaa         <  1:1364939/140‑1 (MQ=255)                                                                                                                           gCCGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAAt        >  1:750547/1‑140 (MQ=255)                                                                                                                           gCCGTGAGGATCAGTTCCAGCAACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGGTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAAt        >  2:112254/1‑140 (MQ=255)                                                                                                                            ccGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATg       <  2:403127/140‑1 (MQ=255)                                                                                                                            ccGTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCCTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATg       <  1:1159201/140‑1 (MQ=255)                                                                                                                              gTGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCt     <  1:655646/140‑1 (MQ=255)                                                                                                                               tGAGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtg    <  1:1128403/140‑1 (MQ=255)                                                                                                                                 aGGATCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtgtg  >  2:1188883/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTG  >  NC_000913/4152567‑4152834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑