Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,295,029	A→G	E183E (GAA→GAG)	gltP →	glutamate/aspartate : H(+) symporter GltP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,295,029	0	A	G	100.0%	295.4 / NA	80	E183E (GAA→GAG)	gltP	glutamate/aspartate : H(+) symporter GltP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/39);  total (41/39)

TTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGT  >  NC_000913/4294896‑4295162                                                                                                                                      |                                                                                                                                      ttATGGGCACGATTTTGTCGCT‑GTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTgg                                                                                                                                <  1:968259/140‑1 (MQ=255)  tATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTgg                                                                                                                                <  2:131727/140‑1 (MQ=255)  tATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTgg                                                                                                                                >  1:467892/1‑140 (MQ=255)  tATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTgg                                                                                                                                <  2:540052/140‑1 (MQ=255)     gggCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGa                                                                                                                             <  2:1038753/140‑1 (MQ=255)       gCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGAcc                                                                                                                           >  2:697348/1‑140 (MQ=255)            tttttGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTTTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGtt                                                                                                                      <  1:1233217/139‑1 (MQ=255)            aTTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGtt                                                                                                                      >  2:419367/1‑140 (MQ=255)             ttttGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGGTCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTc                                                                                                                     <  2:1428863/140‑1 (MQ=255)             ttttGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTc                                                                                                                     <  1:271041/140‑1 (MQ=255)             ttttGTCGCTGGTGCCGACGAACATTGCGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGTCCGTGTTc                                                                                                                     <  2:565641/140‑1 (MQ=255)                 gTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCt                                                                                                                 <  1:165303/140‑1 (MQ=255)                   cGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTcc                                                                                                               <  1:765509/140‑1 (MQ=255)                     cTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATACTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCAt                                                                                                             >  2:1312957/1‑140 (MQ=255)                       ggTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCAtct                                                                                                           >  2:755795/1‑140 (MQ=255)                           ccGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGa                                                                                                       >  2:1129547/1‑140 (MQ=255)                              acgaACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAAc                                                                                                    <  1:1013760/140‑1 (MQ=255)                                      tgtgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTa                                                                                            <  1:936036/140‑1 (MQ=255)                                        tgGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTaaa                                                                                          <  1:1354229/140‑1 (MQ=255)                                          gCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGt                                                                                        <  1:931804/140‑1 (MQ=255)                                          gCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGt                                                                                        >  2:154803/1‑140 (MQ=255)                                           cGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTATAgag                                                                                       >  2:863919/1‑138 (MQ=255)                                           cGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTg                                                                                       <  2:1192583/140‑1 (MQ=255)                                           cGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTg                                                                                       <  1:473461/140‑1 (MQ=255)                                            gTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGa                                                                                      <  2:467892/140‑1 (MQ=255)                                             tCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGAc                                                                                     >  1:1536288/1‑140 (MQ=255)                                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                <  2:1341732/140‑1 (MQ=255)                                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                <  1:441252/140‑1 (MQ=255)                                                  ggcgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTcaca                                                                                <  1:726223/140‑1 (MQ=255)                                                    cgaaaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATg                                                                              <  2:149627/140‑1 (MQ=255)                                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTg                                                                           <  1:1202806/140‑1 (MQ=255)                                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTg                                                                           <  1:706230/140‑1 (MQ=255)                                                       aaggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCC‑‑CGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                         <  2:1203439/140‑1 (MQ=255)                                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                         <  1:419367/140‑1 (MQ=255)                                                         ggcgaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGAt                                                                         <  2:692514/140‑1 (MQ=255)                                                            gaaaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCg                                                                      <  1:174082/140‑1 (MQ=255)                                                              aaTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGtt                                                                    <  1:1129547/140‑1 (MQ=255)                                                               aTGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTa                                                                   >  2:754126/1‑140 (MQ=255)                                                                tactgcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTAt                                                                  <  1:1312957/138‑1 (MQ=255)                                                                  ctgcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGc                                                                <  1:188693/140‑1 (MQ=255)                                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                              >  1:971566/1‑140 (MQ=255)                                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                              <  2:416023/140‑1 (MQ=255)                                                                    gcCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAc                                                              >  1:996451/1‑140 (MQ=255)                                                                    gcCGATCATCTTTTTCTCGGTGCTGTCTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGCGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACACACATGGCGATGCGTTaagaac                                                              >  2:910267/1‑135 (MQ=255)                                                                     ccGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCAcc                                                             >  2:1405721/1‑140 (MQ=255)                                                                        atcatcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggt                                                          >  2:327022/1‑140 (MQ=255)                                                                        atcatcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggt                                                          >  1:1276291/1‑140 (MQ=255)                                                                           atcTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggtggg                                                       <  1:700801/140‑1 (MQ=255)                                                                             cTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCggtgggtg                                                     <  1:154803/140‑1 (MQ=255)                                                                                  tctcGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGttt                                                >  2:471707/1‑140 (MQ=255)                                                                                    tcGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTgc                                              <  1:1108652/140‑1 (MQ=255)                                                                                       gTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCt                                           >  2:423017/1‑140 (MQ=255)                                                                                       gTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCAGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGCGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCt                                           >  2:926286/1‑140 (MQ=255)                                                                                        tGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTg                                          >  1:383110/1‑140 (MQ=255)                                                                                          cTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGAt                                        >  1:1136627/1‑140 (MQ=255)                                                                                          cTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGAt                                        >  2:140693/1‑140 (MQ=255)                                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                >  2:632898/1‑140 (MQ=255)                                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                >  1:1527229/1‑140 (MQ=255)                                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                >  2:897430/1‑140 (MQ=255)                                                                                                  tCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGa                                >  2:807715/1‑140 (MQ=255)                                                                                                    tGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGAGACTGACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACg                              <  2:1154923/140‑1 (MQ=255)                                                                                                       tgCTTTCGTCCCTGCCCGCGACGTAACGTGAGCCGCTGGTCACGGTGTTATGCTCCATCCCTGAAACCATGTTTAAAATGACTCACAGGTTGATGCGTTCCGCACCGGTGGGGGTGTTTGCGCTGATTGCGGTGAGGGTg                           >  2:177045/2‑140 (MQ=255)                                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                           >  1:36388/1‑140 (MQ=255)                                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                           >  1:541273/1‑140 (MQ=255)                                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                           >  1:971664/1‑140 (MQ=255)                                                                                                       ggCTTTCTTCCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTg                           >  2:910145/1‑140 (MQ=255)                                                                                                            tcttcCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                      >  1:1483407/1‑140 (MQ=255)                                                                                                            tcttcCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                      >  2:198493/1‑140 (MQ=255)                                                                                                            tcttcCCTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTaa                      >  1:1358658/1‑140 (MQ=255)                                                                                                                cccTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACttt                  <  1:807715/140‑1 (MQ=255)                                                                                                                cccTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACttt                  <  2:767118/140‑1 (MQ=255)                                                                                                                  cTGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTgg                >  2:1313429/1‑140 (MQ=255)                                                                                                                   tGCCCGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGt               >  1:456734/1‑140 (MQ=255)                                                                                                                     cccGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGttt             <  1:47679/140‑1 (MQ=255)                                                                                                                     cccGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGttt             >  1:974869/1‑140 (MQ=255)                                                                                                                     cccGCGACACATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGttt             >  2:1462261/1‑140 (MQ=255)                                                                                                                      ccGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTtc            >  2:606174/1‑140 (MQ=255)                                                                                                                      ccGCGACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTtc            >  1:1424940/1‑140 (MQ=255)                                                                                                                        gcgACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTtctc          >  2:123379/1‑140 (MQ=255)                                                                                                                         cgACGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCg         <  1:471707/140‑1 (MQ=255)                                                                                                                           tcGCATCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTTTGTTTGCGCTGATTTCGGTGACGGTGGCTAACTTTGGTTTCTCGtc       <  2:1276291/139‑1 (MQ=255)                                                                                                                               aTCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCtg   >  1:28987/1‑140 (MQ=255)                                                                                                                                tCGTGAGCCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCtgt  >  1:759686/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      TTATGGGCACGATTTTGTCGCTGGTGCCGACGAACATTGTGGCGTCGATGGCGAAAGGCGAAATGCTGCCGATCATCTTTTTCTCGGTGCTGTTTGGTCTGGGGCTTTCTTCCCTGCCCGCGACGCATCGTGAACCGCTGGTGACCGTGTTCCGCTCCATCTCTGAAACCATGTTTAAAGTGACTCACATGGTGATGCGTTATGCACCGGTGGGTGTGTTTGCGCTGATTGCGGTGACGGTGGCTAACTTTGGTTTCTCGTCTCTGT  >  NC_000913/4294896‑4295162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑