Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,807	A→G	T203A (ACC→GCC)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,807	0	A	G	100.0%	69.9 / NA	20	T203A (ACC→GCC)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/8);  total (12/8)

CGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAG  >  NC_000913/283686‑283941                                                                                                                          |                                                                                                                                       cGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCg                                                                                                                      <  1:105632/140‑1 (MQ=255)          gCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACg                                                                                                             <  1:1322908/140‑1 (MQ=255)             gCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGTAGGCCATCGGCGCGCGTTTCGCCAACCACGAAc                                                                                                          <  1:1059121/140‑1 (MQ=255)             gCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAAc                                                                                                          <  1:563556/140‑1 (MQ=255)                cGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAActct                                                                                                       <  1:1512980/140‑1 (MQ=255)                          aTTGCGCTGGCCGCGATGCACGCCCTGCCGACTGTTCTGGTGCCGGGCGGGGCGACGTTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCagga                                                                                             <  2:980680/140‑1 (MQ=255)                             gcgcTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGgc                                                                                          >  2:178206/1‑140 (MQ=255)                               gcTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGgccg                                                                                        <  1:1353229/140‑1 (MQ=255)                                  ggCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGccgccg                                                                                     >  2:1373809/1‑140 (MQ=255)                                   gCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGa                                                                                    >  2:855132/1‑140 (MQ=255)                                                                      gggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCCGCGCCTCGCCGGgcggcg                                                 >  2:1105941/1‑140 (MQ=255)                                                                       ggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGGGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGcggcgg                                                >  2:90334/1‑140 (MQ=255)                                                                           gggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCAGgtgt                                            >  2:1079810/1‑140 (MQ=255)                                                                                      ccgccgACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCgg                                 >  2:7084/1‑140 (MQ=255)                                                                                           gACCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCCGCGCGCCCTTCGCCAACCACGAACTCACCCCGCAGAAGGCCGCCGAACGGCGCTGTCGCGCCTTCGCCTCGCCGGGCGGCGGGTGACAGTTCATCGGCTcgg                            >  2:1075521/1‑140 (MQ=255)                                                                                            aCCGTCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCAcggc                           >  2:644761/1‑140 (MQ=255)                                                                                                tCGGGGAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGc                       >  2:1076619/1‑140 (MQ=255)                                                                                                     gAAGACGCGGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTc                  <  1:178206/140‑1 (MQ=255)                                                                                                           gcgGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCCCCTCGCCggg            >  2:1052753/1‑139 (MQ=255)                                                                                                            cgGGCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCCCGCAgggg           >  2:206532/1‑138 (MQ=255)                                                                                                              ggCAAGGTGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAggtggt         <  1:7084/140‑1 (MQ=255)                                                                                                                     tGCAGGCCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAg  >  1:663826/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                       CGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAG  >  NC_000913/283686‑283941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑