BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,377,043	A→G	T57A (ACG→GCG)	gdx →	guanidinium exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,377,043	0	A	G	99.1%	397.0 / ‑4.0	108	T57A (ACG→GCG)	gdx	guanidinium exporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (67/40); minor base T (1/0); total (68/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 115 total aligned reads displayed.

CTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGACGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAA  >  NC_000913/4376908‑4377177

ctgGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGggcggc                                                                                                                                    >  2:685575/1‑140 (MQ=255)

ctgGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGggcggc                                                                                                                                    <  1:1217803/140‑1 (MQ=255)

ctgGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGggcggc                                                                                                                                    <  1:1262086/140‑1 (MQ=255)

     aGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATg                                                                                                                               <  1:1288946/140‑1 (MQ=255)

         gTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCgt                                                                                                                           >  1:528698/1‑140 (MQ=255)

          tATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCgtg                                                                                                                          >  2:1205498/1‑140 (MQ=255)

                ccGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACg                                                                                                                    >  1:1413714/1‑140 (MQ=255)

                   ttGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATTGCGTCACTTGCCGGGGCGATGAACTGGCTACCAGCCAGGGTAGCTTATGCCGCGTGGACAGGt                                                                                                                 >  2:1060974/1‑140 (MQ=255)

                   ttGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACgggg                                                                                                                 >  2:697411/1‑139 (MQ=255)

                   ttGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGt                                                                                                                 >  2:1179531/1‑140 (MQ=255)

                   ttGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGt                                                                                                                 >  1:923278/1‑140 (MQ=255)

                        cTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTgg                                                                                                            >  1:496478/1‑140 (MQ=255)

                          gAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCTTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGgcg                                                                                                          <  2:1161402/140‑1 (MQ=255)

                           aaaTATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGgcgc                                                                                                         >  2:1052601/1‑140 (MQ=255)

                                taCCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCg                                                                                                    >  2:1514841/1‑140 (MQ=255)

                                taCCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCg                                                                                                    >  1:246821/1‑140 (MQ=255)

                                taCCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCg                                                                                                    >  2:712684/1‑140 (MQ=255)

                                 aCCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCgg                                                                                                   >  1:465853/1‑140 (MQ=255)

                                 aCCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCgg                                                                                                   >  1:423415/1‑140 (MQ=255)

                                  cccACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTTCTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGgc                                                                                                  <  2:120199/140‑1 (MQ=255)

                                     aCGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGcgcg                                                                                               >  1:728101/1‑140 (MQ=255)

                                     aCGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGcgcg                                                                                               >  1:137804/1‑140 (MQ=255)

                                     aCGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGcgcg                                                                                               >  1:1539573/1‑140 (MQ=255)

                                       ggCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGc                                                                                             >  1:713261/1‑140 (MQ=255)

                                       ggCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGc                                                                                             >  1:325570/1‑140 (MQ=255)

                                       ggCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGc                                                                                             >  2:1494860/1‑140 (MQ=255)

                                       ggCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGc                                                                                             >  2:897451/1‑140 (MQ=255)

                                       ggCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGc                                                                                             >  1:1188025/1‑140 (MQ=255)

                                        gCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGcc                                                                                            >  1:210886/1‑140 (MQ=255)

                                        gCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGcc                                                                                            <  2:772447/140‑1 (MQ=255)

                                             aGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTTTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAAc                                                                                       <  1:1135447/140‑1 (MQ=255)

                                             aGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAAc                                                                                       >  2:233927/1‑140 (MQ=255)

                                             aGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAAc                                                                                       <  2:561087/140‑1 (MQ=255)

                                             aGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAAc                                                                                       <  2:219694/140‑1 (MQ=255)

                                             aGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAAc                                                                                       <  2:1376533/140‑1 (MQ=255)

                                               tCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCg                                                                                     <  1:886430/140‑1 (MQ=255)

                                                  tttGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCa                                                                                  <  2:1474282/140‑1 (MQ=255)

                                                   ttGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCAt                                                                                 >  1:48869/1‑140 (MQ=255)

                                                   ttGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCAt                                                                                 >  2:217501/1‑140 (MQ=255)

                                                        gCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGtgc                                                                            >  1:907520/1‑140 (MQ=255)

                                                          cGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGtgctg                                                                          <  1:685575/140‑1 (MQ=255)

                                                                  attaCTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGa                                                                  <  1:1060974/140‑1 (MQ=255)

                                                                   ttaCTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAg                                                                 <  1:1133729/140‑1 (MQ=255)

                                                                     aCTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTc                                                               <  2:1147749/140‑1 (MQ=255)

                                                                       tgtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCg                                                             <  1:1514841/140‑1 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  1:1258269/1‑140 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  2:882046/1‑140 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  1:1332762/1‑140 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  1:1513320/1‑140 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  1:240048/1‑140 (MQ=255)

                                                                        gtgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGc                                                            >  2:379316/1‑140 (MQ=255)

                                                                         tgACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCt                                                           >  1:1470754/1‑140 (MQ=255)

                                                                          gACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTa                                                          >  2:427995/1‑140 (MQ=255)

                                                                          gACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTa                                                          >  1:892234/1‑140 (MQ=255)

                                                                          gACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTa                                                          >  1:778557/1‑140 (MQ=255)

                                                                           aCGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTaa                                                         >  2:557171/1‑140 (MQ=255)

                                                                           aCGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTaa                                                         >  1:1340025/1‑140 (MQ=255)

                                                                           aCGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTaa                                                         >  2:207242/1‑140 (MQ=255)

                                                                           aCGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTaa                                                         >  2:738224/1‑140 (MQ=255)

                                                                             ggCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAAcc                                                       >  2:63408/1‑140 (MQ=255)

                                                                                gatgatTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGTCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGa                                                    <  2:388218/140‑1 (MQ=255)

                                                                                gatgatTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGa                                                    <  2:496478/140‑1 (MQ=255)

                                                                                gatgatTGTCAGTATGGCGCCACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCTGTGAGTCCGCTAACCCGa                                                    <  2:475039/140‑1 (MQ=255)

                                                                                   gatTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATgc                                                 >  1:1147443/1‑140 (MQ=255)

                                                                                       gTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCt                                             >  2:121942/1‑140 (MQ=255)

                                                                                          ggTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGc                                          <  2:689203/139‑1 (MQ=255)

                                                                                          aGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGc                                          >  1:593629/1‑140 (MQ=255)

                                                                                            tATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGa                                        >  1:1006148/1‑140 (MQ=255)

                                                                                              tGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGt                                      <  2:1413714/140‑1 (MQ=255)

                                                                                                gcgcTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCt                                    <  1:1385595/140‑1 (MQ=255)

                                                                                                 cgcTACTTGCCTGGGCGTTGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTACTGGCGCAGTCGGCGCGGCCATAACCGGCCTTGTGCTGCTCGGTGAGTCCGCTAACACGATGCGCCTGGCGAGTCTg                                   >  2:646552/1‑140 (MQ=255)

                                                                                                 cgcTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTg                                   >  1:159986/1‑140 (MQ=255)

                                                                                                 cgcTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTg                                   >  2:367112/1‑140 (MQ=255)

                                                                                                 cgcTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTg                                   >  2:562109/1‑140 (MQ=255)

                                                                                                 cgcTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTg                                   >  2:420716/1‑140 (MQ=255)

                                                                                                     aCTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGt                               <  1:1115462/140‑1 (MQ=255)

                                                                                                     aCTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGt                               >  1:1037311/1‑140 (MQ=255)

                                                                                                     aCTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGt                               <  2:388171/140‑1 (MQ=255)

                                                                                                      cTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGtt                              <  2:553562/140‑1 (MQ=255)

                                                                                                      cTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGtt                              >  2:1091713/1‑140 (MQ=255)

                                                                                                      cTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGtt                              >  2:242185/1‑140 (MQ=255)

                                                                                                      cTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGtt                              >  1:1276662/1‑140 (MQ=255)

                                                                                                      cTTGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGtt                              >  2:1049583/1‑140 (MQ=255)

                                                                                                        tGCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTaa                            <  2:178541/140‑1 (MQ=255)

                                                                                                         gcgtGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGGGCAGTCGGCGCGGCGATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGAGAGTCTGACGTTAAt                           <  2:1349921/137‑1 (MQ=255)

                                                                                                         gCCTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAAt                           >  2:609561/1‑140 (MQ=255)

                                                                                                           cTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCg                         <  2:325570/140‑1 (MQ=255)

                                                                                                           cTGGGCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCg                         >  2:1433584/1‑140 (MQ=255)

                                                                                                              ggCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTAt                      <  2:210886/140‑1 (MQ=255)

                                                                                                              ggCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTAt                      >  2:76089/1‑140 (MQ=255)

                                                                                                               gCGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCTGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTAtt                     <  1:295203/140‑1 (MQ=255)

                                                                                                                cGATGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTg                    <  2:1329407/140‑1 (MQ=255)

                                                                                                                  aTGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTggg                  <  2:528698/140‑1 (MQ=255)

                                                                                                                  aTGAAATCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTggg                  <  1:217501/140‑1 (MQ=255)

                                                                                                                     aaaTCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGat               <  1:233927/140‑1 (MQ=255)

                                                                                                                        tCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGattat            <  1:508353/140‑1 (MQ=255)

                                                                                                                        tCGTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGattat            <  1:169513/140‑1 (MQ=255)

                                                                                                                          gTTACCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTg          <  2:921908/140‑1 (MQ=255)

                                                                                                                             aCCAGTAGGGGCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTc       <  2:1513320/140‑1 (MQ=255)

                                                                                                                                  tAGGGTCGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGaaa  >  1:1159348/1‑140 (MQ=255)

CTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCTGGGCGATGAAATCGTTACCAGTAGGGACGGCTTATGCCGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAA  >  NC_000913/4376908‑4377177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`