BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,466,573	A→G	V225A (GTT→GCT)	treR ←	DNA‑binding transcriptional repressor TreR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,466,573	0	A	G	100.0%	388.0 / NA	104	V225A (GTT→GCT)	treR	DNA‑binding transcriptional repressor TreR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (48/56); total (48/56)

Only 100 of 107 total aligned reads displayed.

TCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATA  >  NC_000913/4466436‑4466709

tCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCg                                                                                                                                        <  1:385904/140‑1 (MQ=255)

     aaCGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTtctc                                                                                                                                   <  2:352014/140‑1 (MQ=255)

       cGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCAt                                                                                                                                 <  1:837951/140‑1 (MQ=255)

       cGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCAt                                                                                                                                 >  1:630394/1‑140 (MQ=255)

       cGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCAt                                                                                                                                 >  2:1415934/1‑140 (MQ=255)

         gCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAg                                                                                                                               <  1:428050/140‑1 (MQ=255)

         gCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAg                                                                                                                               <  1:865296/140‑1 (MQ=255)

            tATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCt                                                                                                                            <  2:21033/140‑1 (MQ=255)

            tATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCt                                                                                                                            >  1:1108848/1‑140 (MQ=255)

                    aCGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCAt                                                                                                                    >  1:1120749/1‑140 (MQ=255)

                    aCGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCAt                                                                                                                    >  1:171718/1‑140 (MQ=255)

                    aCGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCAt                                                                                                                    >  1:1131941/1‑140 (MQ=255)

                    aCGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCAt                                                                                                                    >  2:1395727/1‑140 (MQ=255)

                    aCGCTCGCCAGTTGCAAGGCGTCGATGCGTTGCTCTTCCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGCTGCGCACAGTAAGGCGGTCGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATATCCTTGCTTCAt                                                                                                                    >  2:338012/1‑140 (MQ=255)

                     cGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATa                                                                                                                   >  1:827248/1‑140 (MQ=255)

                      gCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAg                                                                                                                  <  2:1497111/140‑1 (MQ=255)

                      gCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAg                                                                                                                  >  2:1209283/1‑140 (MQ=255)

                       cTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGc                                                                                                                 <  1:1395727/140‑1 (MQ=255)

                         cGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCaa                                                                                                               >  1:1189710/1‑140 (MQ=255)

                                tGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTgg                                                                                                        <  2:343258/140‑1 (MQ=255)

                                 gCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGc                                                                                                       <  2:425525/140‑1 (MQ=255)

                                   aaGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAg                                                                                                     <  2:1186838/140‑1 (MQ=255)

                                    aGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAgg                                                                                                    >  2:352574/1‑140 (MQ=255)

                                    aGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAgg                                                                                                    >  2:770158/1‑140 (MQ=255)

                                       tgtCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGggcg                                                                                                 >  1:580143/1‑140 (MQ=255)

                                         tCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGggcggc                                                                                               >  1:1388605/1‑140 (MQ=255)

                                         tCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGggcggc                                                                                               >  1:1475501/1‑140 (MQ=255)

                                         tCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGggcggc                                                                                               >  2:320360/1‑140 (MQ=255)

                                                 ttGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGAt                                                                                       <  2:564580/140‑1 (MQ=255)

                                                 ttGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGAt                                                                                       >  1:497308/1‑140 (MQ=255)

                                                 ttGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGAt                                                                                       >  2:877053/1‑140 (MQ=255)

                                                 ttGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGAt                                                                                       >  2:711914/1‑140 (MQ=255)

                                                 ttGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGAt                                                                                       >  2:1381756/1‑140 (MQ=255)

                                                   gCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGc                                                                                     <  1:927183/140‑1 (MQ=255)

                                                   gCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGc                                                                                     <  2:608370/140‑1 (MQ=255)

                                                             gTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATgcg                                                                           <  2:1189710/140‑1 (MQ=255)

                                                             gTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATgcg                                                                           <  1:1471656/140‑1 (MQ=255)

                                                               aTTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCt                                                                         >  2:1354598/1‑140 (MQ=255)

                                                                tttACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCtt                                                                        >  2:1094164/1‑140 (MQ=255)

                                                                   aCTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGc                                                                     >  2:1125111/1‑140 (MQ=255)

                                                                    cTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCa                                                                    <  1:1147028/140‑1 (MQ=255)

                                                                    cTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCa                                                                    <  2:1120749/140‑1 (MQ=255)

                                                                     ttGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAg                                                                   <  1:805379/140‑1 (MQ=255)

                                                                        cgcCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAAc                                                                >  2:1221691/1‑140 (MQ=255)

                                                                        cgcCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAAc                                                                >  1:467888/1‑140 (MQ=255)

                                                                        cgcCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAAc                                                                <  2:1005493/140‑1 (MQ=255)

                                                                          ccAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGc                                                              >  1:256866/1‑140 (MQ=255)

                                                                            aaGTGCCAGCGTGTCGGTTGCGCATAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCa                                                            >  2:709235/1‑140 (MQ=255)

                                                                             aGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAg                                                           >  2:1208294/1‑140 (MQ=255)

                                                                              gTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAgg                                                          <  2:1108848/140‑1 (MQ=255)

                                                                              gTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAgg                                                          <  2:862039/140‑1 (MQ=255)

                                                                                  cAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAgg                                                      >  2:1307092/1‑140 (MQ=255)

                                                                                        gtCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGt                                                <  1:471644/140‑1 (MQ=255)

                                                                                         tCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTg                                               >  1:6697/1‑140 (MQ=255)

                                                                                         tCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTg                                               >  2:1355438/1‑140 (MQ=255)

                                                                                          cGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGa                                              <  1:1234867/140‑1 (MQ=255)

                                                                                          cGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGa                                              <  2:630394/140‑1 (MQ=255)

                                                                                            gTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACg                                            <  1:768499/140‑1 (MQ=255)

                                                                                            gTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACg                                            <  1:131702/140‑1 (MQ=255)

                                                                                             ttGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGt                                           <  2:904426/140‑1 (MQ=255)

                                                                                                cgcACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGc                                        >  1:1424258/1‑140 (MQ=255)

                                                                                                  cacaGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCtt                                      >  2:1245805/1‑140 (MQ=255)

                                                                                                  cacaGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCtt                                      >  1:1333653/1‑140 (MQ=255)

                                                                                                     aGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTAcc                                   >  1:737927/1‑140 (MQ=255)

                                                                                                     aGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTAcc                                   >  2:1003190/1‑140 (MQ=255)

                                                                                                      gTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCg                                  <  1:1354598/140‑1 (MQ=255)

                                                                                                      gTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCg                                  <  2:1411541/140‑1 (MQ=255)

                                                                                                       tAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCgg                                 <  1:1221691/140‑1 (MQ=255)

                                                                                                        aaGGCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGt                                <  1:1125111/140‑1 (MQ=255)

                                                                                                          ggCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTg                              >  2:1326202/1‑140 (MQ=255)

                                                                                                          ggCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTg                              <  2:827248/140‑1 (MQ=255)

                                                                                                          ggCGGTAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTg                              <  2:1002759/140‑1 (MQ=255)

                                                                                                               tAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacg                         <  1:1094164/140‑1 (MQ=255)

                                                                                                               tAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacg                         >  1:1215652/1‑140 (MQ=255)

                                                                                                               tAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacg                         >  1:1065671/1‑140 (MQ=255)

                                                                                                               tAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacg                         >  2:1246624/1‑140 (MQ=255)

                                                                                                               tAGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacg                         <  1:255223/140‑1 (MQ=255)

                                                                                                                aGTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacgt                        <  1:1218604/140‑1 (MQ=255)

                                                                                                                 gTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacgtc                       >  2:1348028/1‑140 (MQ=255)

                                                                                                                 gTTTCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacgtc                       >  2:1111170/1‑140 (MQ=255)

                                                                                                                  tttCAGGCGTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtcacgtca                      <  1:1209283/140‑1 (MQ=255)

                                                                                                                          gTAATCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCgg              <  1:1539723/140‑1 (MQ=255)

                                                                                                                            aaTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCa            >  1:1180969/1‑140 (MQ=255)

                                                                                                                             aTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGGCGTCGCTTACCGGTTGTCACGTCACTGTGCGGccc           <  2:728489/140‑3 (MQ=255)

                                                                                                                             aTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtca                           <  1:238624/124‑1 (MQ=255)

                                                                                                                             aTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTgtca                           >  2:238624/1‑124 (MQ=255)

                                                                                                                             aTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCAc           <  2:438258/140‑1 (MQ=255)

                                                                                                                             aTCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCAc           <  2:1328349/140‑1 (MQ=255)

                                                                                                                              tCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACg          <  2:638055/140‑1 (MQ=255)

                                                                                                                              tCACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACg          <  2:737927/140‑1 (MQ=255)

                                                                                                                               cACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGc         <  1:1208294/140‑1 (MQ=255)

                                                                                                                               cACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGc         <  2:359631/140‑1 (MQ=255)

                                                                                                                               cACTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGc         <  2:1388605/140‑1 (MQ=255)

                                                                                                                                 cTTTTGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCg       <  1:1415934/140‑1 (MQ=255)

                                                                                                                                  ttttGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCga      <  1:711914/140‑1 (MQ=255)

                                                                                                                                   tttGCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCgag     >  2:284045/1‑140 (MQ=255)

                                                                                                                                      gCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATa  <  2:797632/140‑1 (MQ=255)

                                                                                                                                      gCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATa  <  2:580143/140‑1 (MQ=255)

                                                                                                                                      gCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATa  <  2:492120/140‑1 (MQ=255)

                                                                                                                                      gCAGCGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATa  <  2:334078/140‑1 (MQ=255)

TCATTAACGGCGTATTACCGACGCTCGCCAGTTGCAAGGTGTCGATGCGTTGCTCTTGCAGGTATTTACTTGCGCCAAGTGCCAGCGTGTCGGTTGCGCACAGTAAGGCGGTAGTTTCAGGCGTAATCACTTTTGCAACGTTCTCATAGCCTTGCTTCATAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATA  >  NC_000913/4466436‑4466709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`