Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	337,584	A→G	E269E (GAA→GAG)	yahF →	putative acyl‑CoA synthetase YahF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,584	0	A	G	100.0%	149.0 / NA	41	E269E (GAA→GAG)	yahF	putative acyl‑CoA synthetase YahF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

AGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGA  >  NC_000913/337459‑337720                                                                                                                              |                                                                                                                                         aGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTg                                                                                                                            >  1:1533790/1‑140 (MQ=255)     agaAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGc                                                                                                                        >  1:862299/1‑140 (MQ=255)       aaaaTCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCaa                                                                                                                      >  1:1170355/1‑140 (MQ=255)           tCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCg                                                                                                                  >  1:1021691/1‑140 (MQ=255)              gcggcCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCggtg                                                                                                               <  1:1390936/140‑1 (MQ=255)                   cTGAGGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCTTTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGgtcgt                                                                                                          <  2:10180/140‑1 (MQ=255)                      atgatgCTCGACGCCATCGGGATGCTGGAAAACGATCCGCCAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGTGCGCGCCTGCCGCAAGCCGGTGGTCGTCTg                                                                                                       <  1:1294685/140‑1 (MQ=255)                      atgatgCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTg                                                                                                       <  1:144204/140‑1 (MQ=255)                       tgatgCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACGGAAATCATTGCCCTTATCTCCAATCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGc                                                                                                      <  2:703196/140‑1 (MQ=255)                                aCGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGAt                                                                                             >  1:521742/1‑140 (MQ=255)                                 cGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATc                                                                                            >  2:356198/1‑140 (MQ=255)                                   cctgcGGGATGCTGGAAAACGATCCGTAAACTGAAATCATTGCGTTTATCCCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGt                                                                                          <  2:275875/136‑1 (MQ=255)                                     aTCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTgg                                                                                        >  2:1055431/1‑140 (MQ=255)                                          gATGCTGGAAACCGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGaaa                                                                                   <  2:128186/140‑1 (MQ=255)                                             gCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGc                                                                                <  1:75098/140‑1 (MQ=255)                                              cTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc                                                                               <  2:874643/140‑1 (MQ=255)                                              cTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc                                                                               >  1:1403511/1‑140 (MQ=255)                                                ggAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAg                                                                             <  1:150588/140‑1 (MQ=255)                                                        aTCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAg                                                                     >  1:1187430/1‑140 (MQ=255)                                                                        cATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTg                                                     <  1:356198/140‑1 (MQ=255)                                                                                          aCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAgg                                   >  2:1099175/1‑140 (MQ=255)                                                                                           ccGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc                                  <  2:1115509/140‑1 (MQ=255)                                                                                            cgcctgcgcctgCGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCAGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCa                                 >  2:1158058/1‑140 (MQ=255)                                                                                             gcctgcgcctgcGGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAg                                >  2:1251920/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  1:1508564/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  2:1341398/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  2:1319862/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  1:1202684/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  1:1253620/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  2:799117/1‑140 (MQ=255)                                                                                              cctgcgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                               >  1:446672/1‑140 (MQ=255)                                                                                                  cgcctgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTa                           <  2:1533790/140‑1 (MQ=255)                                                                                                      tgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAg                       >  2:1358956/1‑140 (MQ=255)                                                                                                      tgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGATCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAg                       >  1:1093642/1‑140 (MQ=255)                                                                                                      cgcgGTGGCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAg                       <  2:1187430/139‑1 (MQ=255)                                                                                                             gCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGAt                >  2:697263/1‑140 (MQ=255)                                                                                                             gCCCGCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGAt                <  1:1055431/140‑1 (MQ=255)                                                                                                              ccggCAAAGTGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATg               <  2:1256714/137‑1 (MQ=255)                                                                                                                       tGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGc      >  1:1131341/1‑140 (MQ=255)                                                                                                                       tGCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGc      >  1:1073862/1‑140 (MQ=255)                                                                                                                        gCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCg     <  2:521742/140‑1 (MQ=255)                                                                                                                        gCTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCg     <  1:216582/140‑1 (MQ=255)                                                                                                                         cTGGAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGt    >  1:501645/1‑140 (MQ=255)                                                                                                                           ggAGCGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGa  <  1:1403396/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         AGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGA  >  NC_000913/337459‑337720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑