Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,183	A→G	Y185C (TAC→TGC)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,183	0	A	G	100.0%	291.5 / NA	78	Y185C (TAC→TGC)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/43);  total (35/43)

AGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGG  >  NC_000913/86048‑86317                                                                                                                                         |                                                                                                                                       gggTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTG‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  2:848268/139‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  1:1041642/140‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  1:101050/140‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  1:34532/140‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  2:148822/140‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  2:1385730/140‑1 (MQ=255) aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAAt                                                                                                                                    <  1:511557/140‑1 (MQ=255)     gCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCa                                                                                                                                <  1:585568/140‑1 (MQ=255)      cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTTGGTCAGTATGCGTTCTTGCAATCCCac                                                                                                                               >  1:709581/1‑140 (MQ=255)      cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCac                                                                                                                               <  1:293986/140‑1 (MQ=255)      cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCac                                                                                                                               <  1:1063676/140‑1 (MQ=255)      cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCac                                                                                                                               <  2:91488/140‑1 (MQ=255)      cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCac                                                                                                                               >  1:1494549/1‑140 (MQ=255)           aaaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCActacta                                                                                                                          <  2:75706/140‑1 (MQ=255)            aaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCActacta                                                                                                                          <  2:1017418/140‑1 (MQ=255)            aaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACtactac                                                                                                                         <  2:599871/140‑1 (MQ=255)            aaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACtactac                                                                                                                         <  2:1349059/140‑1 (MQ=255)                cTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCa                                                                                                                                      >  2:1415573/1‑123 (MQ=255)                cTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGa                                                                                                                     >  1:1410665/1‑140 (MQ=255)                    ctggctggCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATa                                                                                                                 <  2:555386/140‑1 (MQ=255)                         tggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAggg                                                                                                            <  1:694177/140‑1 (MQ=255)                         tggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAggg                                                                                                            <  2:1215708/140‑1 (MQ=255)                              gcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAAt                                                                                                       >  2:121982/1‑140 (MQ=255)                                     gTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGt                                                                                                >  1:802373/1‑140 (MQ=255)                                      tCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTg                                                                                               <  1:1140020/140‑1 (MQ=255)                                      gcGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTg                                                                                               <  2:1354817/139‑1 (MQ=255)                                       cGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGc                                                                                              <  1:1522210/140‑1 (MQ=255)                                       cGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTCGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGc                                                                                              >  2:443470/1‑140 (MQ=255)                                        gCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGct                                                                                             <  2:1321767/140‑1 (MQ=255)                                           cAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTg                                                                                          <  1:1471123/140‑1 (MQ=255)                                             ggACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCa                                                                                        <  2:1494549/140‑1 (MQ=255)                                             ggACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCa                                                                                        <  1:128480/140‑1 (MQ=255)                                                   gtagtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCt                                                                                  >  2:815012/1‑140 (MQ=255)                                                    tagtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTg                                                                                 >  1:825038/1‑140 (MQ=255)                                                    tagtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTg                                                                                 >  2:570790/1‑140 (MQ=255)                                                         gTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGc                                                                            >  2:39948/1‑140 (MQ=255)                                                         gTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGc                                                                            >  2:518771/1‑140 (MQ=255)                                                           tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCgg                                                                          >  2:90120/1‑140 (MQ=255)                                                                    gAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAAc                                                                 <  1:410017/140‑1 (MQ=255)                                                                      aaGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTGCTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCg                                                               <  1:812609/140‑1 (MQ=255)                                                                      aaGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCg                                                               <  2:160007/140‑1 (MQ=255)                                                                       aGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAACACCgg                                                              >  2:933453/1‑140 (MQ=255)                                                                       aGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCgg                                                              <  1:819609/140‑1 (MQ=255)                                                                       aGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCgg                                                              <  1:1101812/140‑1 (MQ=255)                                                                       aGATATTCTTAATCCGGCGAACAAATAA‑CCCTATGTCTGGCCGGAGTCGGACAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGGAGCGGCAAAAAAACCgg                                                              >  2:1431580/1‑140 (MQ=255)                                                                        gATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTGCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGt                                                             <  2:248168/140‑1 (MQ=255)                                                                          tatTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTg                                                           <  2:72455/140‑1 (MQ=255)                                                                           atTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGt                                                          <  1:324403/140‑1 (MQ=255)                                                                                 aaTCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGt                                                    >  1:777614/1‑140 (MQ=255)                                                                                 aaTCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGt                                                    >  1:1455346/1‑140 (MQ=255)                                                                                  aTCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTa                                                   <  1:698309/140‑1 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              >  1:11119/1‑140 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              >  1:1228627/1‑140 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              >  1:1297500/1‑140 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              >  1:535839/1‑140 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              <  1:1419157/140‑1 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCgg                                              >  1:400779/1‑140 (MQ=255)                                                                                       gCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGGAGCGGCAAAAAAAACGGTTGTCTACGTAGGCgg                                              >  2:749508/1‑140 (MQ=255)                                                                                        cGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCggg                                             >  2:910599/1‑139 (MQ=255)                                                                                        cGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGt                                             >  1:1384580/1‑140 (MQ=255)                                                                                        cGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGt                                             <  1:121982/140‑1 (MQ=255)                                                                                           aCAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTggg                                          <  1:1266548/140‑1 (MQ=255)                                                                                                 tA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATc                                    >  1:1287/1‑140 (MQ=255)                                                                                                         tGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCAcggcgg                             <  1:1039029/140‑1 (MQ=255)                                                                                                          gTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCggg                            <  2:442902/140‑1 (MQ=255)                                                                                                          gTCTGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCggg                            >  2:1233241/1‑140 (MQ=255)                                                                                                          gTCTGGCAGGAGTCGGTCAGTATGCGTTCTTGCAATCCCCCTACTCCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCggg                            <  2:182619/140‑1 (MQ=255)                                                                                                             tGGCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTg                         <  1:449238/140‑1 (MQ=255)                                                                                                              ggCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGc                        >  2:1156176/1‑140 (MQ=255)                                                                                                               gCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGccggc                       >  1:671221/1‑136 (MQ=255)                                                                                                               gCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGcc                       >  2:1450361/1‑140 (MQ=255)                                                                                                               gCCGGAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGcc                       >  2:1270393/1‑140 (MQ=255)                                                                                                                   gAGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCCGCCCTcc                   >  2:814598/1‑139 (MQ=255)                                                                                                                    aGTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATcag                  >  1:395639/1‑140 (MQ=255)                                                                                                                     gTCGGTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATcagc                 <  2:11119/140‑1 (MQ=255)                                                                                                                         gTCAGTATGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGtt             >  1:460966/1‑140 (MQ=255)                                                                                                                                tGCGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGCCTACGTAGGCGGCGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaagaa      >  2:1190679/1‑140 (MQ=255)                                                                                                                                  cGTTCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAAc    <  1:281160/140‑1 (MQ=255)                                                                                                                                    ttCTTGCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGGTGGAAGAAAAgg  >  2:311565/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTA‑CCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGG  >  NC_000913/86048‑86317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑