Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	894,476	C→T	N231N (AAC→AAT)	potF →	putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	894,476	0	C	T	100.0%	270.3 / NA	77	N231N (AAC→AAT)	potF	putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/31);  total (46/31)

CTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGTTCT  >  NC_000913/894346‑894612                                                                                                                                   |                                                                                                                                         cTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCa                                                                                                                                 <  1:1065691/140‑1 (MQ=255) cTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCa                                                                                                                                 <  2:717420/140‑1 (MQ=255)      gtgtTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACgg                                                                                                                            <  2:20033/140‑1 (MQ=255)      gtgtTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTACCATTCATCTCAATACATTAATGACCTGGCAAACgg                                                                                                                            >  2:78680/1‑140 (MQ=255)          tGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGat                                                                                                                        <  1:188184/140‑1 (MQ=255)          tGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGat                                                                                                                        <  1:646272/140‑1 (MQ=255)                aTCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGc                                                                                                                  >  1:875309/1‑140 (MQ=255)                 tctcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCg                                                                                                                 >  2:470996/1‑140 (MQ=255)                 tctcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCg                                                                                                                 >  2:1103798/1‑140 (MQ=255)                   tcGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTc                                                                                                               >  2:1413511/1‑140 (MQ=255)                        aaaGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTAt                                                                                                          <  2:339687/140‑1 (MQ=255)                            aTCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGc                                                                                                      <  1:636321/140‑1 (MQ=255)                                    aGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAgg                                                                                              <  1:635829/140‑1 (MQ=255)                                      cACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTggg                                                                                                  >  1:1072874/1‑134 (MQ=255)                                      cACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTggg                                                                                                  <  2:1072874/134‑1 (MQ=255)                                      cACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTg                                                                                            >  1:949589/1‑140 (MQ=255)                                        cTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGAt                                                                                          >  1:408147/1‑140 (MQ=255)                                        cTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGAt                                                                                          >  1:238277/1‑140 (MQ=255)                                        cTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGAt                                                                                          >  1:263546/1‑140 (MQ=255)                                             gCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTg                                                                                     >  1:359166/1‑140 (MQ=255)                                               ggATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGc                                                                                   <  2:1063730/140‑1 (MQ=255)                                                   gatTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCCGGTAATGTTTGGCAggg                                                                               >  2:786243/1‑139 (MQ=255)                                                        cacCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCaa                                                                          >  2:317062/1‑140 (MQ=255)                                                           cggaccggCAAAAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCTGGTGATGTCTGGCAGGCGTCAAAcc                                                                       >  1:886453/1‑140 (MQ=255)                                                             gaccggCAACAGATCTGCTGTTAAAGCTGCGTCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACcgc                                                                     >  2:171060/1‑140 (MQ=255)                                                             gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGGTGTCTGGCAGGCGTCAAACcgc                                                                     >  2:1154523/1‑140 (MQ=255)                                                             gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGAAGTCTGGCAGGCGTCAAACcgc                                                                     >  1:587198/1‑140 (MQ=255)                                                             gaccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGGGATGTCTGGCAGGCGTCAAACcgg                                                                     >  1:510643/1‑139 (MQ=255)                                                              gccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAGCATTCGTTATTTCCATTCATCTCTATACATTAATGACCTGGCAAACGGCGACATTTGCGCCGCTATCGGCTGGGCCGGAGTGGTTTGGCCGGCGGCAAAcccgg                                                                    >  2:211448/2‑137 (MQ=255)                                                              accggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACcgcg                                                                    <  1:439708/140‑1 (MQ=255)                                                               ccggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACcgcgc                                                                   >  1:299753/1‑140 (MQ=255)                                                                cggCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACcgcgcg                                                                  >  2:652494/1‑140 (MQ=255)                                                                  gCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaa                                                                >  1:622487/1‑140 (MQ=255)                                                                  gCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaa                                                                >  2:1056448/1‑140 (MQ=255)                                                                  gCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCCTGCAAGCGTTAAACcgacctga                                                                >  2:446875/1‑134 (MQ=255)                                                                   cAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaag                                                               <  1:447425/140‑1 (MQ=255)                                                                   cAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaag                                                               >  2:674652/1‑140 (MQ=255)                                                                    aaCAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg                                                              <  1:1103798/140‑1 (MQ=255)                                                                    aaCAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg                                                              <  1:883101/140‑1 (MQ=255)                                                                      cAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaaggaa                                                            <  2:1170513/140‑1 (MQ=255)                                                                         aTCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCg                                                         <  1:470996/140‑1 (MQ=255)                                                                          tCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCga                                                        <  2:1039526/140‑1 (MQ=255)                                                                            tgctgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag                                                      >  2:7321/1‑140 (MQ=255)                                                                              ctgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa                                                    >  1:635479/1‑140 (MQ=255)                                                                              ctgTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa                                                    <  1:989390/140‑1 (MQ=255)                                                                                gTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGGCAAACCGCGCGGAGGAAGCgaagaaag                                                  >  2:104982/1‑138 (MQ=255)                                                                                 ttAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATgg                                                 >  1:1255179/1‑140 (MQ=255)                                                                                 ttAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATgg                                                 <  2:408147/140‑1 (MQ=255)                                                                                  tAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGGCTGGCAGGCGTCCAACCGCGCGAAGGAAGCGAAGAATggg                                                >  2:585909/1‑139 (MQ=255)                                                                                   aaaGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCg                                               <  1:1413511/140‑1 (MQ=255)                                                                                         gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAGGCGAAGAATGGCGTGAATg                                         >  1:1157037/1‑140 (MQ=255)                                                                                         gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATg                                         >  1:790332/1‑140 (MQ=255)                                                                                         gcgcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATg                                         >  1:1402967/1‑140 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATggc                                       >  2:72092/1‑138 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGtc                                       >  1:787375/1‑140 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGtc                                       <  2:294128/140‑1 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGtc                                       <  2:238277/140‑1 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGtc                                       <  2:679159/140‑1 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAAAGtc                                       >  2:1318299/1‑140 (MQ=255)                                                                                           gcCCGAACATTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGGGGTGTCTGGCAGGCGTCCAACCGCGCGAAGGGAGCGAAGAATGGCGTGGATGtt                                       >  1:1480235/1‑139 (MQ=255)                                                                                                   aTTCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTtctc                               <  1:786243/140‑1 (MQ=255)                                                                                                     tCGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGa                             >  1:593626/1‑140 (MQ=255)                                                                                                      cGTTATTTCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGAt                            <  1:1506984/140‑1 (MQ=255)                                                                                                             tCCATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATTGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCaaaa                     >  1:77307/1‑140 (MQ=255)                                                                                                               cATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaaga                   >  2:381456/1‑140 (MQ=255)                                                                                                               cATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaaga                   >  1:1146528/1‑140 (MQ=255)                                                                                                               cATTCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaaga                   <  1:182993/140‑1 (MQ=255)                                                                                                                 ttCATCTCAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaagaag                 >  2:646819/1‑140 (MQ=255)                                                                                                                      ctcAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGGGAAGAATGGCGTGAATGTCTCGTTTTCGGTTCCAAAAGGAGGGGCg            >  2:1260050/1‑140 (MQ=255)                                                                                                                      ctcAATACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGGCTGGCAAGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCg            >  2:1488509/1‑140 (MQ=255)                                                                                                                          aTACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATgg        <  1:652494/140‑1 (MQ=255)                                                                                                                           tACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGc       <  1:785737/140‑1 (MQ=255)                                                                                                                           tACATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGc       <  2:622487/140‑1 (MQ=255)                                                                                                                            aCATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCg      <  1:699269/140‑1 (MQ=255)                                                                                                                            aCATTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCg      <  1:78680/140‑1 (MQ=255)                                                                                                                              aTTAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGtt    >  1:60145/1‑140 (MQ=255)                                                                                                                                tAATGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGTTCt  >  1:194542/1‑140 (MQ=255)                                                                                                                                tAATGACCTGGCAAACGGCGATATTTACGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGGCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGTTCt  >  2:1290759/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                         CTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGCACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAACATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGTTCT  >  NC_000913/894346‑894612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑