Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	101,615	T→C	V284A (GTC→GCC)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	101,615	0	T	C	100.0%	275.7 / NA	75	V284A (GTC→GCC)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/35);  total (40/35)

TGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCG  >  NC_000913/101480‑101741                                                                                                                                        |                                                                                                                               tGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCAcc                                                                                                                            <  2:1245833/140‑1 (MQ=255)   ttACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCt                                                                                                                          >  1:1277105/1‑140 (MQ=255)   ttACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCt                                                                                                                          >  1:418076/1‑140 (MQ=255)         cgAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATgc                                                                                                                    >  2:157185/1‑140 (MQ=255)         cgAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATgc                                                                                                                    >  2:1043421/1‑140 (MQ=255)           aGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATgcgc                                                                                                                  >  2:973022/1‑140 (MQ=255)               gggCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAgg                                                                                                              <  2:1504370/140‑1 (MQ=255)               gggCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAgg                                                                                                              <  1:1338723/140‑1 (MQ=255)               gggCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAgg                                                                                                              <  1:85198/140‑1 (MQ=255)               gggCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAgg                                                                                                              <  1:343681/140‑1 (MQ=255)                  cGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGgtcg                                                                                                           >  2:354614/1‑140 (MQ=255)                      aGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCAt                                                                                                       >  1:371302/1‑140 (MQ=255)                              aCTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCt                                                                                               >  1:1150000/1‑140 (MQ=255)                                   cGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAAcg                                                                                          >  1:772291/1‑140 (MQ=255)                                    ggCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAAcgc                                                                                         <  1:738061/140‑1 (MQ=255)                                      cTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAAcgccg                                                                                       <  2:1328851/140‑1 (MQ=255)                                         cAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAg                                                                                    >  2:649864/1‑140 (MQ=255)                                         cAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAg                                                                                    >  2:1464796/1‑140 (MQ=255)                                         cAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAg                                                                                    >  1:543575/1‑140 (MQ=255)                                          aGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGc                                                                                   >  1:198753/1‑140 (MQ=255)                                          aGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGc                                                                                   <  1:281997/140‑1 (MQ=255)                                               aGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCtgcgg                                                                              <  1:49253/140‑1 (MQ=255)                                               aGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCtgcgg                                                                              >  1:864418/1‑140 (MQ=255)                                                    ccGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCtgcggttgcg                                                                         <  1:1368109/140‑1 (MQ=255)                                                     cGACGTGCGAGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGGGCCACGCTGAATTCGCGAGGTCGTCAGAACGCGCTGAACGCCGCAGCtgcggttgcgg                                                                        >  2:607981/1‑140 (MQ=255)                                                      gACGTGCGTGTAGAAGATTATCAGTAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCtgcggttgcggt                                                                       <  2:823384/140‑1 (MQ=255)                                                      gACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCtgcggttgcggt                                                                       <  1:171004/140‑1 (MQ=255)                                                        cgtgcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGcggttgcggttg                                                                     <  2:1150000/140‑1 (MQ=255)                                                           gcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTa                                                                  >  2:286384/1‑140 (MQ=255)                                                           gcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTa                                                                  >  2:776264/1‑140 (MQ=255)                                                           gcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTa                                                                  <  2:727870/140‑1 (MQ=255)                                                           gcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTa                                                                  <  2:383942/140‑1 (MQ=255)                                                           gcgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTa                                                                  <  1:354614/140‑1 (MQ=255)                                                            cgtgTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTAc                                                                 >  1:91012/1‑140 (MQ=255)                                                              tgtAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACgg                                                               >  2:645009/1‑140 (MQ=255)                                                                  gaagaTTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAaga                                                           <  1:157185/140‑1 (MQ=255)                                                                   aagaTTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAagag                                                          <  1:144433/140‑1 (MQ=255)                                                                    agaTTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAgg                                                         >  2:591747/1‑140 (MQ=255)                                                                     gaTTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAggg                                                        <  1:1281206/140‑1 (MQ=255)                                                                      aTTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGc                                                       >  2:580296/1‑140 (MQ=255)                                                                          tCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTg                                                   >  1:916107/1‑140 (MQ=255)                                                                          tCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTg                                                   >  2:801555/1‑140 (MQ=255)                                                                              cagATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTgacga                                               <  2:1240172/140‑1 (MQ=255)                                                                              cagATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTgacga                                               <  2:418076/140‑1 (MQ=255)                                                                              cagATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTgacga                                               <  1:1459624/140‑1 (MQ=255)                                                                              cagATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTgacga                                               <  2:674507/140‑1 (MQ=255)                                                                              cagATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTgacga                                               <  1:821587/140‑1 (MQ=255)                                                                                gATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGacgacg                                             >  2:336897/1‑140 (MQ=255)                                                                                gATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGacgacg                                             >  2:1104249/1‑140 (MQ=255)                                                                                 aTTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGAcgacga                                            >  2:308143/1‑140 (MQ=255)                                                                                 aTTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGAcgacga                                            >  2:1301202/1‑140 (MQ=255)                                                                                   tGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAgg                                          >  1:101275/1‑140 (MQ=255)                                                                                   tGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAgg                                          <  1:1275680/140‑1 (MQ=255)                                                                                           aGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTg                                  >  2:583146/1‑140 (MQ=255)                                                                                             gggCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGAGCATTGACGACGAGGCTATTTTGCg                                >  1:1370002/1‑140 (MQ=255)                                                                                              ggCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCgg                               <  2:864418/140‑1 (MQ=255)                                                                                               gCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCggg                              >  2:397768/1‑140 (MQ=255)                                                                                               gCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACCAGGCTATTTTGCggg                              >  2:1269557/1‑140 (MQ=255)                                                                                                       cGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGaa                      >  2:16226/1‑140 (MQ=255)                                                                                                        gctgctGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGaaa                     <  2:926356/140‑1 (MQ=255)                                                                                                        gctgctGCGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGaaa                     <  1:1469513/140‑1 (MQ=255)                                                                                                          tgctgcGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGc                   <  2:543575/140‑1 (MQ=255)                                                                                                          tgctgcGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGc                   <  2:980197/140‑1 (MQ=255)                                                                                                          tgctgcGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCACTTGAAAGc                   >  2:630097/1‑140 (MQ=255)                                                                                                             tgcGCCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGAATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTc                >  2:1424564/1‑140 (MQ=255)                                                                                                               cgcCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCa              >  2:1210239/1‑140 (MQ=255)                                                                                                               cgcCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCa              <  2:371302/140‑1 (MQ=255)                                                                                                               cgcCAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCa              >  2:255372/1‑140 (MQ=255)                                                                                                                  cAGGACAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAggg           <  2:101275/140‑1 (MQ=255)                                                                                                                      aCAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACt       >  1:1308631/1‑140 (MQ=255)                                                                                                                      aCAAAGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACt       >  1:397985/1‑140 (MQ=255)                                                                                                                         aaGAGCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGt    >  2:347721/1‑140 (MQ=255)                                                                                                                          agagCCGATGCGCGCCACCCTGAAGGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTc   >  2:1138227/1‑140 (MQ=255)                                                                                                                           gagCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCg  <  2:1256352/140‑1 (MQ=255)                                                                                                                           gagCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCg  <  1:1464796/140‑1 (MQ=255)                                                                                                                           gagCCGATGCGCGCCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCg  <  2:790519/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               TGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCG  >  NC_000913/101480‑101741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑