Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,274,761	T→C	S326S (AGT→AGC)	ychO →	putative invasin YchO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,274,761	0	T	C	100.0%	306.4 / NA	82	S326S (AGT→AGC)	ychO	putative invasin YchO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/40);  total (42/40)

GTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGTCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGC  >  NC_000913/1274624‑1274894                                                                                                                                          |                                                                                                                                      gtgCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAagcca                                                                                                                                     <  1:1200429/140‑1 (MQ=255)   gCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAagccagt                                                                                                                                   <  2:841753/140‑1 (MQ=255)       ttAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGtt                                                                                                                               >  1:273308/1‑140 (MQ=255)          gTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACg                                                                                                                            <  1:341288/140‑1 (MQ=255)          gTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACg                                                                                                                            <  1:196871/140‑1 (MQ=255)            cACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTg                                                                                                                          >  2:1183012/1‑140 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAGTCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  1:1323097/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTTCCGCTTTGGTGTTCCGCTCAAAAACCAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:547883/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       >  1:1048641/1‑140 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       >  2:919845/1‑140 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:717887/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:633604/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:500842/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:182590/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:1325305/140‑1 (MQ=255)               tgtgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGACTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTa                                                                                                                       <  2:652152/140‑1 (MQ=255)                 tgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGt                                                                                                                     <  2:813468/140‑1 (MQ=255)                 tgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGt                                                                                                                     <  2:763965/140‑1 (MQ=255)                 tgACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTCTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGt                                                                                                                     <  1:1403372/140‑1 (MQ=255)                    cGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGc                                                                                                                  >  1:674811/1‑140 (MQ=255)                      gCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGTTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTa                                                                                                                >  1:1433267/1‑140 (MQ=255)                      gCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTa                                                                                                                <  1:1419741/140‑1 (MQ=255)                       cccAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTAt                                                                                                               >  2:1395372/1‑140 (MQ=255)                           gCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACa                                                                                                           >  2:754525/1‑140 (MQ=255)                                   aGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAg                                                                                                   <  1:156870/140‑1 (MQ=255)                                     ggTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCg                                                                                                 >  1:405576/1‑140 (MQ=255)                                     ggTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCg                                                                                                 >  1:1053563/1‑140 (MQ=255)                                     ggTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCg                                                                                                 <  2:1027079/140‑1 (MQ=255)                                     ggTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCg                                                                                                 >  1:54303/1‑140 (MQ=255)                                      gtgtAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  2:547907/1‑140 (MQ=255)                                      gTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  2:1258438/1‑140 (MQ=255)                                      gTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  1:90658/1‑140 (MQ=255)                                      gTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  1:603097/1‑140 (MQ=255)                                      gTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  2:484053/1‑140 (MQ=255)                                      gTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGa                                                                                                >  2:649532/1‑140 (MQ=255)                                          aaGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAaata                                                                                            >  2:190248/1‑140 (MQ=255)                                            gTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAaataat                                                                                          <  2:1412742/140‑1 (MQ=255)                                             tGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATc                                                                                         >  2:8429/1‑140 (MQ=255)                                               gCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTa                                                                                       >  1:1528273/1‑140 (MQ=255)                                               gCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTa                                                                                       >  1:635020/1‑140 (MQ=255)                                                cGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTAc                                                                                      <  2:273057/140‑1 (MQ=255)                                                 gAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTAcc                                                                                     <  2:1491460/140‑1 (MQ=255)                                                   aaaTCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGa                                                                                   <  1:754525/140‑1 (MQ=255)                                                   aaaTCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGa                                                                                   <  1:456942/140‑1 (MQ=255)                                                      tCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGActc                                                                                <  2:571224/140‑1 (MQ=255)                                                       cAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGActct                                                                               <  1:1258438/140‑1 (MQ=255)                                                       cAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGActct                                                                               <  2:674811/140‑1 (MQ=255)                                                       cAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGActct                                                                               <  1:782235/140‑1 (MQ=255)                                                                cTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCg                                                                      <  2:273308/140‑1 (MQ=255)                                                                         aaTCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGaaa                                                             <  1:568173/140‑1 (MQ=255)                                                                         aaTCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGaaa                                                             >  1:604323/1‑140 (MQ=255)                                                                               aaTTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGtt                                                       <  2:640278/140‑1 (MQ=255)                                                                                aTTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTa                                                      <  1:484053/140‑1 (MQ=255)                                                                                 ttACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTaa                                                     <  1:471702/140‑1 (MQ=255)                                                                                   aCCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACg                                                   <  2:1040147/140‑1 (MQ=255)                                                                                   aCCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACg                                                   >  1:1423195/1‑140 (MQ=255)                                                                                   aCCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACg                                                   <  1:8429/140‑1 (MQ=255)                                                                                   aCCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTACCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACg                                                   <  2:1157916/140‑1 (MQ=255)                                                                                     cGCTTTGGTGTACCACTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGgt                                                 >  1:267998/1‑140 (MQ=255)                                                                                        tttGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGtt                                              <  1:611797/140‑1 (MQ=255)                                                                                         ttGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGttt                                             >  1:458689/1‑140 (MQ=255)                                                                                             tgtACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTgg                                         <  1:1395372/140‑1 (MQ=255)                                                                                               tACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCg                                       <  1:1028246/140‑1 (MQ=255)                                                                                                aCCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGa                                      >  1:1246772/1‑140 (MQ=255)                                                                                                aCCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGa                                      <  2:54303/140‑1 (MQ=255)                                                                                                  cGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGaca                                    >  1:1482295/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:80353/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:1432226/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  2:745168/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:581508/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:694510/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:730629/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:791558/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:830015/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:831474/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  1:952245/1‑140 (MQ=255)                                                                                                    cTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAACCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAcc                                  >  2:324927/1‑140 (MQ=255)                                                                                                     tCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAccg                                 <  1:190248/140‑1 (MQ=255)                                                                                                      cAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAccgc                                >  1:1439365/1‑140 (MQ=255)                                                                                                       aaaaaaCAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAccgcc                               <  1:1326063/140‑1 (MQ=255)                                                                                                       aaaaaaCAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACAccgcc                               >  1:322539/1‑140 (MQ=255)                                                                                                          aaaCAACTTTCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTg                            <  2:604323/140‑1 (MQ=255)                                                                                                                 tttCTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCACTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTa                     >  2:501793/1‑140 (MQ=255)                                                                                                                    cTGCGGGCGAGGTTGCCGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTaaaa                  <  2:405576/140‑1 (MQ=255)                                                                                                                                   ccGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTg   >  2:1368704/1‑140 (MQ=255)                                                                                                                                    cGAAAGCCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGc  >  2:1325725/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGAAAGTCAGTCGTTACGTGGTAGTCGCTATGACAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGCGAAAAACGTTAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGC  >  NC_000913/1274624‑1274894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑