Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,148	A→G	L254P (CTG→CCG)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,148	0	A	G	100.0%	256.9 / NA	71	L254P (CTG→CCG)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/36);  total (35/36)

CCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCAT  >  NC_000913/1360011‑1360283                                                                                                                                          |                                                                                                                                        ccGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCggg                                                                                                                                       >  2:605543/1‑140 (MQ=255)  cgcgTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGc                                                                                                                                      <  1:1315566/140‑1 (MQ=255)    cgTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGt                                                                                                                                    >  1:665514/1‑140 (MQ=255)          aaaGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATACAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGt                                                                                                                              >  1:483449/1‑140 (MQ=255)          aaaGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGt                                                                                                                              <  1:643762/140‑1 (MQ=255)          aaaGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGt                                                                                                                              >  1:1237497/1‑140 (MQ=255)            aGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGc                                                                                                                            <  2:704754/140‑1 (MQ=255)               aCGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAg                                                                                                                         <  1:1196568/140‑1 (MQ=255)               aCGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAg                                                                                                                         <  1:340517/140‑1 (MQ=255)                   tctcGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGca                                                                                                                     >  2:419105/1‑140 (MQ=255)                   tctcGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCCTATGCTCTTCTGCCATCAGACCCACCGGGCGTTTTAGTGCCAGCGca                                                                                                                     >  2:778832/1‑140 (MQ=255)                               tattTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCt                                                                                                         >  2:492426/1‑140 (MQ=255)                                     gCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGc                                                                                                   <  1:658994/140‑1 (MQ=255)                                     gCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGc                                                                                                   <  2:62947/140‑1 (MQ=255)                                     gCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGc                                                                                                   <  1:1136474/140‑1 (MQ=255)                                     gCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGc                                                                                                   <  1:780697/140‑1 (MQ=255)                                      cATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCa                                                                                                  >  2:149385/1‑140 (MQ=255)                                      cATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCa                                                                                                  >  2:33606/1‑140 (MQ=255)                                       aTACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCTATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCAc                                                                                                 <  1:605543/140‑1 (MQ=255)                                       aTACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCAc                                                                                                 <  2:401321/140‑1 (MQ=255)                                        tACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACg                                                                                                >  1:550869/1‑140 (MQ=255)                                          cTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGtt                                                                                              <  2:104926/140‑1 (MQ=255)                                           tGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTa                                                                                             >  2:1522963/1‑140 (MQ=255)                                            gctatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCACCCTATGCCTTTCTGCCATCAGACGCACCGGGCGTTTTGGTGCCAACGCACCATCGCAGGGTTCCAGCACCTTAt                                                                                            >  2:1022139/3‑140 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGGCATAAAAGGGGCGGGCCTCTTATGGTTTTCTGCCATCAGACGCCCCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  2:1261668/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  1:725827/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  1:768885/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  2:507761/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  2:294651/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  1:1100090/140‑1 (MQ=255)                                            gatatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTAt                                                                                            <  1:612254/140‑1 (MQ=255)                                             atatggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATc                                                                                           >  2:34893/1‑140 (MQ=255)                                                tggatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCCGACGCCCCGGGCGTTTTAGGGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGt                                                                                        >  2:852420/1‑140 (MQ=255)                                                  gatatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTAt                                                                                      <  2:440909/140‑1 (MQ=255)                                                   atatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATg                                                                                     >  2:53925/1‑140 (MQ=255)                                                   atatgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATg                                                                                     >  2:800247/1‑140 (MQ=255)                                                     atgCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCCTCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGt                                                                                   >  2:1199916/1‑140 (MQ=255)                                                       gCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTaa                                                                                 >  1:619625/1‑140 (MQ=255)                                                       gCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTaa                                                                                 >  2:374856/1‑140 (MQ=255)                                                           tCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGGAAAGGt                                                                             >  2:363774/1‑140 (MQ=255)                                                             cGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTg                                                                           >  1:1384423/1‑140 (MQ=255)                                                                  cccGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTc                                                                      >  2:844962/1‑140 (MQ=255)                                                                          ctctTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGAc                                                              <  1:419105/140‑1 (MQ=255)                                                                          ctctTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGATTGATTTCAAACTGAc                                                              <  2:121010/140‑1 (MQ=255)                                                                            ctTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGAccc                                                            >  1:564208/1‑140 (MQ=255)                                                                                aTACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGCTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCg                                                        <  1:1383898/140‑1 (MQ=255)                                                                                  aCGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGCCCCGGCGAg                                                      <  2:1384423/140‑1 (MQ=255)                                                                                     gCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGcc                                                   >  1:891687/1‑140 (MQ=255)                                                                                         cGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCCTCAGACGCACCGGGCGTTTTAGGGCCCGCGCATCATCGCGGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAg                                               >  2:1213683/1‑140 (MQ=255)                                                                                         cGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAg                                               >  2:580679/1‑140 (MQ=255)                                                                                         cGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAg                                               >  1:1088715/1‑140 (MQ=255)                                                                                         cGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAg                                               >  1:55148/1‑140 (MQ=255)                                                                                         cGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAg                                               >  1:1083998/1‑140 (MQ=255)                                                                                                      gCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATc                                  >  1:604764/1‑140 (MQ=255)                                                                                                         tGCATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGc                               >  1:644145/1‑140 (MQ=255)                                                                                                          gCATCTTATGCTTTTCTGCCCTCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGcc                              >  1:51244/1‑140 (MQ=255)                                                                                                           cATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCg                             >  2:640440/1‑140 (MQ=255)                                                                                                           cATCTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCg                             >  2:243298/1‑140 (MQ=255)                                                                                                              cTTATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGaa                          <  1:181919/140‑1 (MQ=255)                                                                                                               ttATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAAt                         <  1:53925/140‑1 (MQ=255)                                                                                                                tATGCTTTTCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATc                        <  1:374856/140‑1 (MQ=255)                                                                                                                        tCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTaa                <  2:891687/140‑1 (MQ=255)                                                                                                                        tCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTaa                >  1:220042/1‑140 (MQ=255)                                                                                                                        tCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTaa                >  1:199785/1‑140 (MQ=255)                                                                                                                        tCTGCCATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTaa                >  2:796568/1‑140 (MQ=255)                                                                                                                            ccATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTgc            >  1:382485/1‑140 (MQ=255)                                                                                                                             cATCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTgcg           <  1:1263250/140‑1 (MQ=255)                                                                                                                              aTCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTgcgc          <  1:345933/140‑1 (MQ=255)                                                                                                                              aTCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTgcgc          <  1:410324/140‑1 (MQ=255)                                                                                                                               tCAGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGcc         <  1:1522963/140‑1 (MQ=255)                                                                                                                                 aGACGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTACCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAg       <  1:492426/140‑1 (MQ=255)                                                                                                                                   aCGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGtt     <  2:644145/140‑1 (MQ=255)                                                                                                                                   aCGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGATAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGtt     <  2:550869/140‑1 (MQ=255)                                                                                                                                    cGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTtc    <  1:33606/140‑1 (MQ=255)                                                                                                                                    cGCACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTtc    <  1:34893/140‑1 (MQ=255)                                                                                                                                      cACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGTCATCTGCCGGAATCAGCTGTAACTGCGCCAGTtcat  <  2:639982/140‑1 (MQ=255)                                                                                                                                      cACCGGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTtcat  >  1:987785/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACGTTATCAGTATGGTAAAGGTTGATTTCAAACTGACCCGGCGAGGCCTCAGCGACCGCGCCATCTGCCGGAATCAGCTGTAACTGCGCCAGTTCAT  >  NC_000913/1360011‑1360283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑