Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,397,844	T→C	H260R (CAC→CGC)	uspE ←	universal stress protein with a role cellular motility

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,844	0	T	C	100.0%	331.8 / NA	88	H260R (CAC→CGC)	uspE	universal stress protein with a role cellular motility
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (60/28);  total (60/28)

CTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCT  >  NC_000913/1397714‑1397980                                                                                                                                    |                                                                                                                                         cTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCa                                                                                                                                 >  2:108152/1‑140 (MQ=255)  tGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCaa                                                                                                                                >  1:923861/1‑140 (MQ=255)    gTCGGGTTTAATGACCAGCGGGTCGCAGCGAAGATGATCAATCCCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGCCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              <  2:1384760/140‑1 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACTGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  2:146829/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:412096/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  2:606042/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  2:416717/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:1110947/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  2:313068/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:457831/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:1524476/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:958952/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGCGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:1100991/1‑140 (MQ=255)    gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATAACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAAt                                                                                                                              >  1:1265671/1‑140 (MQ=255)        ggTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAgg                                                                                                                          >  2:1065684/1‑140 (MQ=255)         gTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGa                                                                                                                         >  1:499397/1‑140 (MQ=255)            tAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATc                                                                                                                      >  1:1076761/1‑140 (MQ=255)              aTGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCAc                                                                                                                    >  2:222525/1‑140 (MQ=255)                   cagcagGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCtctt                                                                                                               >  2:108456/1‑140 (MQ=255)                   cagcagGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCtctt                                                                                                               >  1:1137478/1‑140 (MQ=255)                    agcagGTCGTAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCtcttc                                                                                                              >  2:204342/1‑140 (MQ=255)                    agcagGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCAGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCtcttc                                                                                                              >  1:860470/1‑140 (MQ=255)                        ggTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGGGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGc                                                                                                          <  1:1311430/140‑1 (MQ=255)                         gTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCa                                                                                                         >  2:1032536/1‑140 (MQ=255)                          tCGCAGCGAAGATGATCAATCCCCTGTC‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGCCCTCCCGTGCCCAGACCCACACCCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAg                                                                                                        <  2:901162/140‑1 (MQ=255)                            gcggCGAAGATGATCAA‑CCCCTGTTCCCCCCGTGTTGCCGGGAAATGCTGCTAAAATCCGGTTGCGCCCTACCGTCCCCAGACCCCCAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAc                                                                                                      <  2:1437662/137‑1 (MQ=255)                             cAGCGAAGATGACCAATCCCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAcc                                                                                                     <  1:1143303/140‑1 (MQ=255)                                   aGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTc                                                                                               >  2:573094/1‑140 (MQ=255)                                     atgatCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTa                                                                                             >  1:516253/1‑140 (MQ=255)                                        atcaatccCCTGTC‑CCGCCGTGTCGCCGAGGAAGGCTGCTGAAATCCCGGTGCCCCCTACCGTCCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGt                                                                                          <  2:806620/140‑1 (MQ=255)                                            atcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTTTGGCAGACCTTTTTCTACGTGCGt                                                                                      >  2:509697/1‑140 (MQ=255)                                            atcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGt                                                                                      >  1:147179/1‑140 (MQ=255)                                            atcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGt                                                                                      >  2:797599/1‑140 (MQ=255)                                             tcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                     >  2:192/1‑140 (MQ=255)                                             tcaCCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                     >  2:1101683/1‑140 (MQ=255)                                             cccccTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                                     <  1:1280334/137‑1 (MQ=255)                                                  tGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGtt                                                                                >  1:226884/1‑140 (MQ=255)                                                       ccgccgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCa                                                                            >  1:1095896/1‑140 (MQ=255)                                                       ccgccgTGTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGCCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCa                                                                            <  2:1180183/140‑1 (MQ=255)                                                        cgccgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAt                                                                           >  2:879516/1‑140 (MQ=255)                                                          ccgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTa                                                                         >  2:1214096/1‑140 (MQ=255)                                                           cgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTaa                                                                        <  2:402978/140‑1 (MQ=255)                                                           cgTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTaa                                                                        >  1:196055/1‑140 (MQ=255)                                                            gtgtTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAAt                                                                       >  2:93272/1‑140 (MQ=255)                                                               ttGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGcc                                                                    >  2:169529/1‑140 (MQ=255)                                                               ttGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGcc                                                                    >  1:638684/1‑140 (MQ=255)                                                                       gAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCt                                                            >  2:1222020/1‑140 (MQ=255)                                                                          tgctgcTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                         >  2:189765/1‑140 (MQ=255)                                                                          tgctgcTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                         >  1:654229/1‑140 (MQ=255)                                                                          tgctgcTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                         >  1:759532/1‑140 (MQ=255)                                                                           gctgcaAAAATCCCGGTGCGTCCTACTGTCCCCAGAACCACACCCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATG‑TTTCATTAATGCCGAATTTCTGCCGc                                                       <  2:457831/140‑1 (MQ=255)                                                                            ctgctgAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGc                                                       >  2:163277/1‑140 (MQ=255)                                                                               ctgAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGGTTTCATTAATGCCGAATTTCTGCCGCAgg                                                    >  1:803520/1‑140 (MQ=255)                                                                                        cGGTGCGTCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCAtt                                           <  1:416717/140‑1 (MQ=255)                                                                                         ggTGCGTCCTCCCGCGCCAAGACCCCCACCCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTg                                          <  2:396766/140‑1 (MQ=255)                                                                                         ggTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTg                                          >  1:986381/1‑140 (MQ=255)                                                                                         ggTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTg                                          >  2:1169639/1‑140 (MQ=255)                                                                                            gCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCa                                       >  1:221932/1‑140 (MQ=255)                                                                                             cGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAg                                      <  1:192/140‑1 (MQ=255)                                                                                                  tACCGTGCCCAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAAt                                 >  1:1129915/1‑140 (MQ=255)                                                                                                        gCCCAGACCCACACCCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGcc                           <  1:108456/140‑1 (MQ=255)                                                                                                         cccAGAACCACACTCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTTTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGccc                          <  1:509697/140‑1 (MQ=255)                                                                                                         cccAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGccc                          >  1:27665/1‑140 (MQ=255)                                                                                                          ccAGAACCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCa                         >  1:1504738/1‑140 (MQ=255)                                                                                                              ccccgcaATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCGCCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGaa                     <  2:1100991/135‑1 (MQ=255)                                                                                                              aaCCACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGaa                     >  2:1387301/1‑140 (MQ=255)                                                                                                                ccACAATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATg                   >  2:66191/1‑140 (MQ=255)                                                                                                                  ccaACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGTCCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc                 <  1:252078/139‑1 (MQ=255)                                                                                                                  ccaACCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc                 <  1:1512149/139‑1 (MQ=255)                                                                                                                  acaATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc                 <  2:1110947/140‑1 (MQ=255)                                                                                                                  acaATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc                 <  2:759532/140‑1 (MQ=255)                                                                                                                  acaATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc                 <  1:1065684/140‑1 (MQ=255)                                                                                                                   ccaacgccGCCTGTAAGCGCTCCGCCAAATCAGGAATCTCCTCTTCTGGCAGATCTTTTTCTACGTGCGTCATGTGTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                <  2:923861/134‑1 (MQ=255)                                                                                                                   caccccccGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                <  2:365097/136‑1 (MQ=255)                                                                                                                   caATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCTCACGAATGGCa                >  1:1494147/1‑140 (MQ=255)                                                                                                                   caATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                >  1:381432/1‑140 (MQ=255)                                                                                                                   caATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                >  1:287004/1‑140 (MQ=255)                                                                                                                   caATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                <  2:1076761/140‑1 (MQ=255)                                                                                                                   caATCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCa                >  1:281262/1‑140 (MQ=255)                                                                                                                    tacctccGCCTGTAGGCGCTCCGCCAAATCAGGAATCTCCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGGCAGCAAATGTTGCCCACGAATGGCAt               <  2:28460/135‑1 (MQ=255)                                                                                                                    aacccccGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCAt               <  1:214162/137‑1 (MQ=255)                                                                                                                    aaTCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCAt               >  1:125609/1‑140 (MQ=255)                                                                                                                    aaTCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCAt               <  1:169529/140‑1 (MQ=255)                                                                                                                    aaTCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCAt               <  1:146829/140‑1 (MQ=255)                                                                                                                     aTCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATc              <  2:516253/140‑1 (MQ=255)                                                                                                                      tCCCCGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCg             >  2:103534/1‑140 (MQ=255)                                                                                                                        cccGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt           >  2:62096/1‑140 (MQ=255)                                                                                                                        cccGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt           >  1:225959/1‑140 (MQ=255)                                                                                                                        cccGCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt           >  1:182440/1‑140 (MQ=255)                                                                                                                           gCCTGTAAGCGCTCCGCCAAATCAGGAATCGCCTCTTCTGGCAGACCTTTTTCTGCGTGCGTCATGTTTTCATTAATGCCGCATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTtata        <  2:1157975/140‑1 (MQ=255)                                                                                                                           gCCTGTAAGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTtata        >  2:84892/1‑140 (MQ=255)                                                                                                                                 aaGCGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCt  <  2:221932/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTT‑CCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCT  >  NC_000913/1397714‑1397980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑